Nonlinear and Non-Gaussian Process Monitoring Based on Simplified R‑Vine Copula

In the field of chemical process monitoring, the vine copula model provides a new idea for describing the interdependence between high-dimensional complex variables, and directly characterizes the correlation without dimensional reduction. However, in actual industrial processes, the number of pair copulas to be optimized and the parameters to be estimated increase rapidly when the dimensionality of the variables is large. This greatly increases the computational load and reduces the detection efficiency. In this paper, a fault diagnosis method based on a simplified R-vine (SRV) model is proposed. Without reducing the precision of the model significantly, the simplified level is set to reduce the complexity of the workload and calculations. The simplified level of an R-vine model is obtained by a Vuong test. Then, the generalized local probability (GLP) of the non-Gaussian state is constructed by using the theory of highest density region (HDR) and a density quantile table. The monitoring results of the Tennessee Eastman (TE) process and a real acetic acid dehydration distillation system show that the proposed SRV approach achieves good performance in monitoring results and computational load for chemical process fault monitoring

Variant in EZR leads to defects in lens development

Congenital cataract is a common cause of blindness. Genetic factors always play important role. This study identified a novel missense variant (c.1412C>T (p.P471L)) in the EZR gene in a four-generation Chinese family with nuclear cataract by linkage analysis and whole-exome sequencing. A knockout study in zebrafish using transcription activator-like effector nucleases was carried out to gain insight into candidate gene function. Conservative and functional prediction suggests that the P-to-L substitution may impair the function of the human ezrin protein. Histology showed developmental delays in the ezrin-mutated zebrafish, manifesting as multilayered lens epithelial cells. Immunohistochemistry revealed abnormal proliferation patterns in mutant fish. The study suggests that ezrin may be involved in the enucleation and differentiation of lens epithelial cells.</p

Polyol-Mediated Solvothermal Synthesis and Electrochemical Performance of Nanostructured V₂O₅ Hollow Microspheres

Hollow vanadyl glycolate nanostructured microspheres were synthesized via a highly scalable and template-free polyol-induced solvothermal process. Subsequent calcination transformed the precursor material into vanadium pentoxide, a well-studied transition metal oxide. The vanadyl glycolate nanoparticles were synthesized through a self-seeding process and then aggregated around N₂ microbubbles formed during the reaction that acted as “quasi-micelles” due to the large polarization discrepancy between nitrogen and water. The proposed formation mechanism provides a firm understanding of the processes leading to the observed hollow microsphere morphology. The thermally treated material was tested as a cathode for lithium-ion battery and showed excellent cycle stability and high rate performance. The exceptional electrochemical performance was attributed to the relatively thin-walled structure that ensured fast phase penetration between the electrolyte and active material and shortened lithium-ion migration distance. The prolonged cycling stability is ascribed to the inherent morphological void that can readily accommodate volume expansion and contraction upon cycling

Supplementary Appendix - Supplemental material for Chinese adult children’s perceived parents’ satisfaction with adult children’s marriage, in-law relationship quality, and adult children’s marital satisfaction

<p>Supplemental material for Chinese adult children’s perceived parents’ satisfaction with adult children’s marriage, in-law relationship quality, and adult children’s marital satisfaction by Hongjian Cao, Mark Fine, Xiaoyi Fang, and Nan Zhou in Journal of Social and Personal Relationships</p

Alterations in the expression of Timeless in LUAD and its clinical significance.

(A) The expression of Timeless in LUAD samples and paired normal samples in TCGA-LUAD. (B) ROC curves measuring the sensitive and specific values of Timeless in TCGA-LUAD. (C) Kaplan-Meier curves of patients with high and low Timeless in TCGA-LUAD (log-rank test). *** p < 0.001.</p

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BackgroundTimeless is well-known for its key role in replication checkpoints. Recent studies reveal the involvement of Timeless and specificity protein (SP) 1 in human malignancies. However, no evidence proved the interaction between SP3 and Timeless in lung adenocarcinoma (LUAD).MethodsThe expression and clinical significance of Timeless were analyzed using the LUAD dataset downloaded from the Cancer Genome Atlas (TCGA). Lentivirus-mediated Timeless knockdown in A549 cells was used to examine the role of Timeless in cell proliferation and pemetrexed (PEM) resistance. Transcription factors (TFs) bound to the Timeless promoter were identified by DNA pull-down technology with HPLC-MS/MS analysis and analyzed by the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway. Dual-luciferase reporter assay was used to determine the activity of SP3 in Timeless transcription.ResultsTimeless was overexpressed in LUAD samples, and it could serve as a potential diagnostic or prognostic biomarker for LUAD patients. shTimeless-mediated knockdown of Timeless reduced cell viability and proliferation and sensitized PEM-resistant A549 cells to PEM. Four fragments (F1: 1–373 bp), (F2: 374–962 bp), (F4: 1274–1645 bp), and (F5: 1646-2000bp) were confirmed as the TF binding profiles of the Timeless promoter. KEGG analysis showed that the TFs bound to the Timeless promoter had relevance to spliceosome, RNA transport, and mRNA surveillance pathways. SP3 promoted the transcription of Timeless via the F2 fragment (374–962 bp) binding motif.ConclusionUpregulation of Timeless mediated by SP3 promotes LUAD cell proliferation, providing evidence to support that targeting the SP3/Timeless axis may be a potential therapeutic strategy against LUAD.</div

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BackgroundTimeless is well-known for its key role in replication checkpoints. Recent studies reveal the involvement of Timeless and specificity protein (SP) 1 in human malignancies. However, no evidence proved the interaction between SP3 and Timeless in lung adenocarcinoma (LUAD).MethodsThe expression and clinical significance of Timeless were analyzed using the LUAD dataset downloaded from the Cancer Genome Atlas (TCGA). Lentivirus-mediated Timeless knockdown in A549 cells was used to examine the role of Timeless in cell proliferation and pemetrexed (PEM) resistance. Transcription factors (TFs) bound to the Timeless promoter were identified by DNA pull-down technology with HPLC-MS/MS analysis and analyzed by the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway. Dual-luciferase reporter assay was used to determine the activity of SP3 in Timeless transcription.ResultsTimeless was overexpressed in LUAD samples, and it could serve as a potential diagnostic or prognostic biomarker for LUAD patients. shTimeless-mediated knockdown of Timeless reduced cell viability and proliferation and sensitized PEM-resistant A549 cells to PEM. Four fragments (F1: 1–373 bp), (F2: 374–962 bp), (F4: 1274–1645 bp), and (F5: 1646-2000bp) were confirmed as the TF binding profiles of the Timeless promoter. KEGG analysis showed that the TFs bound to the Timeless promoter had relevance to spliceosome, RNA transport, and mRNA surveillance pathways. SP3 promoted the transcription of Timeless via the F2 fragment (374–962 bp) binding motif.ConclusionUpregulation of Timeless mediated by SP3 promotes LUAD cell proliferation, providing evidence to support that targeting the SP3/Timeless axis may be a potential therapeutic strategy against LUAD.</div

SP3 increases cell growth by Timeless in A549 cells.

(A) Cell viability was determined by CCK-8 assays in A549 cells after transfection of vec+sh-NC, SP3+sh-NC, or SP3+shTimeless. p-value based on one-way ANOVA followed by Tukey’s comparisons test (n = 3). ** p ## p p-value based on one-way ANOVA followed by Tukey’s comparisons test (n = 3). ** p ## p p-value based on one-way ANOVA followed by Tukey’s comparisons test (n = 3). ** p ## p p-value based on two-way ANOVA with Sidak’s multiple comparison tests (n = 3).</p

Timeless regulates H1299 cell proliferation by modulating SP3.

* p p p # p ## p ### p (TIF)</p

Inhibition of Timeless decreases A549 cell proliferation.

(A) RT-qPCR analysis of the knockdown efficiency of lentiviral-mediated shTimeless on Timeless expression in A549 cells (n = 3). p-value based on a unpaired Student’s t-test. **p p-value based on a unpaired Student’s t-test. **p p-value based on a unpaired Student’s t-test. **p p-value based on a unpaired Student’s t-test. **p p-value based on a unpaired Student’s t-test. **p < 0.05 vs. shNC.</p