Typing of methicillin resistant and sensitive Staphylococcus aureus isolated from Tamilnadu, India using DNA fingerprints by pulsed-field gel electrophoresis

MRSA infection is alarming particularly in hospital set ups/community. We typed 43 isolates of Staphylococcus aureus (MRSA and MSSA) based on genomic DNA restriction fragment length polymorphisms (RFLPs). The genomic DNA of the test isolates was digested with SmaI enzyme, fractionated by PFGE and the patterns were assessed by dendrogram for percentage similarity. The SmaI restricted genomic DNA of 19 MRSA and 24 MSSA identified 27 different PFGE patterns, in which 11 and 16 were from MRSA and MSSA, respectively. Prevalence predominance was observed in few types/subtypes of MSSA (type B and subtype I-1) and MRSA (sub type A-2) and high percentage of similarity was noticed among the subtypes of PFGE types such as P and I of MSSA. During the epidemiological studies, to understand the dissemination of endemic/epidemic MRSA and MSSA, PFGE-based typing of pathogens may be used as a reliable and effective typing method

Expression and production optimization of the cationic antimicrobial peptide : indolicidin by the recombinant E. coli C41 (DE3) clones

The cytoplasmic granules of bovine neutrophils naturally possess indolicidin - a promising cationic antimicrobial peptide as it displays inherent inhibitory activities against a broad type of microbial pathogens. In this study, a shake flask level production and expression optimizations of the indolicidin by the recombinant Escherichia coli C41 (DE3) clones (transformed with pET21a(+) plasmid carrying indolicidin gene) were carried out under standard conditions, as to determine the conditions required for maximal production. It was determined that a concentration of 1 mM of IPTG was effective, the 2×YT with salts and LB media at pH 7.5 with 3-6 h of incubation were required for maximal indolicidin expression

Typing of methicillin resistant and sensitive Staphylococcus aureus isolated from Tamilnadu, India using DNA fingerprints by pulsed-field gel electrophoresis

MRSA infection is alarming particularly in hospital set ups/community. We typed 43 isolates of Staphylococcus aureus (MRSA and MSSA) based on genomic DNA restriction fragment length polymorphisms (RFLPs). The genomic DNA of the test isolates was digested with SmaI enzyme, fractionated by PFGE and the patterns were assessed by dendrogram for percentage similarity. The SmaI restricted genomic DNA of 19 MRSA and 24 MSSA identified 27 different PFGE patterns, in which 11 and 16 were from MRSA and MSSA, respectively. Prevalence predominance was observed in few types/subtypes of MSSA (type B and subtype I-1) and MRSA (sub type A-2) and high percentage of similarity was noticed among the subtypes of PFGE types such as P and I of MSSA. During the epidemiological studies, to understand the dissemination of endemic/epidemic MRSA and MSSA, PFGE-based typing of pathogens may be used as a reliable and effective typing method

Typing of methicillin resistant and sensitive Staphylococcus aureus isolated from Tamilnadu, India using DNA fingerprints by pulsed-field gel electrophoresis

MRSA infection is alarming particularly in hospital set ups/community. We typed 43 isolates of Staphylococcus aureus (MRSA and MSSA) based on genomic DNA restriction fragment length polymorphisms (RFLPs). The genomic DNA of the test isolates was digested with SmaI enzyme, fractionated by PFGE and the patterns were assessed by dendrogram for percentage similarity. The SmaI restricted genomic DNA of 19 MRSA and 24 MSSA identified 27 different PFGE patterns, in which 11 and 16 were from MRSA and MSSA, respectively. Prevalence predominance was observed in few types/subtypes of MSSA (type B and subtype I-1) and MRSA (sub type A-2) and high percentage of similarity was noticed among the subtypes of PFGE types such as P and I of MSSA. During the epidemiological studies, to understand the dissemination of endemic/epidemic MRSA and MSSA, PFGE-based typing of pathogens may be used as a reliable and effective typing method