Mikroprop Agasi Dan Penghasilan Biji Benih Tiruan Anubia Sp.

Shoot tips (0.5 - 1.0 cm) of Allubia nalla were cultured in 1I2MS media containing different combinations of NAA dan BA to produce multiple shoots. AI/ubia nana in 1/2MS medium containing 4 mg/L BA without NAA produced the maximum number of shoots per explant. Shoots produced per explant in 112MS was twice more than in MS but the shoots were smaller in size. Shoot tips cultered in solid medium produced more multiple shoots than in liquid medium. Multiple shoot production of other Anubia species such as Allllhia cOl1gellsis and Al1l1bia lanceolala was also successfhlly done on 112MS + 3.0 mg/L BA. ~rhis showed tbat different species of Anubia can also be propagated using this medium. Rooting of Al1lfbia l7a11a was easy in 1I2MS medium without NAA or BA. Pueuk apeks (0.5 - 1.0 em) Anubia nana dikulturkan di daIam media 112MS dengan pelbagai kombinasi NAA dan BA untuk menentukan media propagasi. Media ] 12MS dengan 3 mg/l BA didapati menghasilkan bilangan pueuk per eksplan yang maksimum. Apabila media MS penuh dengan separuh MS (1I2MS) dibandingkan didapati bilangan pueuk yang dihasilkan pada media 112MS adalah dua kali ganda tetapi saiz pucuknya adalah keci!. Pueuk yang dilkulturkan di dalam media pepejal juga menghasilkan lebih pueuk berbanding dengan media eeeair. Penghasilan pueuk berbilang oleh spesies Anubia yang lain seperti Anubia congensis dan Al1ubia lanceo/ala dientukan. Didapati spesies Anubia yang lain juga dapat dipropagasikan dengan media yang sarna. Penghasilan akar pada pucuk yang dikultur tisu dapat dilakukan dengan mudah pada media 1I2MS tanpa hormon NAA ataupun BA

Data of de novo assembly and functional annotation of transcriptome of Peninsular Malaysian Amomum Roxb. species

The Amomum genus comprises of perennial, tropical, herbaceous plants that are distributed globally and possess both medicinal and ornamental properties. These plants contain a variety of secondary metabolites, including compounds with antioxidant and antimicrobial properties. This is the first transcriptomic analysis of seven Amomum species from Peninsular Malaysia, utilizing leaves, stems, and roots as sample material. Paired-end Illumina HiSeq technology was used for data generation which includes raw data, cleaned reads, de novo assembly, and functional annotation. The data is accessible via NCBI BioProject (PRJNA936673)

The second language acquisition of past tense marker in English by L1 speakers of chinese.

This study is considered the L2 acquisition and underlying of past tense marker, focusing on whether or not L2 learners of English are successful in associating the grammatical properties with Chinese language. Although the dataset is small, the results showed that Chinese speakers are able to acquire the past-tense marker although Chinese language has none of this feature. The L1 Chinese speakers are able to acquire the regular past-tense marker better co mpared to the irregular form

miR-21 Expression and its Correlation with Demographics, Subtypes, and Tumour Suppressor Genes; PTEN and PDCD4 in Breast Cancer Tissues in Malaysia

Objectives: Despite extensive research in breast cancer (BC) genomics, most studies are from Western countries, which do not reflect the multi-ethnic make-up of Malaysia. Hence, microribonucleic acid-21 (miRNA-21), which is known to be an oncogenic stimulator of BC will be investigated by comparing its expression between breast tumour tissues and normal adjacent tissues excised from 67 BC patients, ethnic groups, age groups distribution, neo-adjuvant chemotherapy (NAC) treated and untreated patients, as well as BC subtypes. Methods: Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was used to measure the distribution of miR-21 expression in the paired BC tissues. The expression of the tumour suppressors; PTEN and PDCD4 was also investigated via RT-qPCR and Western Blot for its gene and protein expressions. Results: The results only showed the significance of miR-21 and PTEN expression between the normal adjacent tissues and BC tissues (p<0.05). Additionally, there was a lack of correlation between gene expression of miR-21 against PTEN and PDCD4. Protein expression analysis did not show a significant difference in tumour suppressor proteins; PTEN and PDCD4 expression in both tissue types. Conclusion: miR-21 has a notable presence in BC and is a suitable biomarker to be evaluated further in patients of all ethnicities and age groups