Can ChatGPT Be Considered an Author of a Medical Article?

Kazuki Ide, Philip Hawke, Takeo Nakayama, Can ChatGPT Be Considered an Author of a Medical Article?, Journal of Epidemiology, 2023, Volume 33, Issue 7, Pages 381-382, Released on J-STAGE July 05, 2023, Advance online publication April 08, 2023, Online ISSN 1349-9092, Print ISSN 0917-5040, https://doi.org/10.2188/jea.JE20230030, https://www.jstage.jst.go.jp/article/jea/33/7/33_JE20230030/_article/-char/e

ナノカーボン物質系の電子状態の輸送測定による研究

学位の種別: 課程博士審査委員会委員 : （主査）東京大学准教授 岡本 徹, 東京大学教授 樽茶 清悟, 東京大学教授 島野 亮, 東京大学教授 常行 真司, 東京大学教授 小森 文夫University of Tokyo(東京大学

A Method for Producing Transgenic Cells Using a Multi-Integrase System on a Human Artificial Chromosome Vector

The production of cells capable of expressing gene(s) of interest is important for a variety of applications in biomedicine and biotechnology, including gene therapy and animal transgenesis. The ability to insert transgenes at a precise location in the genome, using site-specific recombinases such as Cre, FLP, and ΦC31, has major benefits for the efficiency of transgenesis. Recent work on integrases from ΦC31, R4, TP901-1 and Bxb1 phages demonstrated that these recombinases catalyze site-specific recombination in mammalian cells. In the present study, we examined the activities of integrases on site-specific recombination and gene expression in mammalian cells. We designed a human artificial chromosome (HAC) vector containing five recombination sites (ΦC31 attP, R4 attP, TP901-1 attP, Bxb1 attP and FRT; multi-integrase HAC vector) and de novo mammalian codon-optimized integrases. The multi-integrase HAC vector has several functions, including gene integration in a precise locus and avoiding genomic position effects; therefore, it was used as a platform to investigate integrase activities. Integrases carried out site-specific recombination at frequencies ranging from 39.3–96.8%. Additionally, we observed homogenous gene expression in 77.3–87.5% of colonies obtained using the multi-integrase HAC vector. This vector is also transferable to another cell line, and is capable of accepting genes of interest in this environment. These data suggest that integrases have high DNA recombination efficiencies in mammalian cells. The multi-integrase HAC vector enables us to produce transgene-expressing cells efficiently and create platform cell lines for gene expression

An Application of the Simulated Annealing to the Calculation of a Combined Mode Choice and Route Choice Network Equilibrium Model with Road Travel Time Uncertainty

金沢大学理工研究域　環境デザイン学系Travel time of public transport such as railway and Light Rail Transit (LRT) is more reliable than road traffic. When assessing the effect of railway transits, the reliability should be considered. Otherwise, the effect may be underestimated. Then, we should not only treat both transit and road users simultaneously and consistently, but also travel time uncertainty of cars and the reliability (or punctuality) of railway for exact evaluation. A network equilibrium model with combined mode and route choice considering road travel time uncertainty does not necessarily have a unique solution, but may have many local solutions, because of the interaction between modes. The simulated annealing is one of the useful techniques to such a problem, to the calculation of the network equilibrium model with combined mode and route choice. In this paper, we apply the network equilibrium model proposed to the Kanazawa urban area using the simulated annealing technique

Semi-dynamic traffic assignment model with mode and route choices under stochastic travel times

Transportation network conditions vary significantly during the course of a day. In many urban areas, public transit and (private) automobiles constitute the actual forms of transportation that use such networks. Public transportation by rail is more reliable than by automobiles or buses; therefore, ordinary static and deterministic traffic assignment models with combined mode and route choices may not be suitable to assess a transportation network that includes public railways. Moreover, within-day dynamics and reliability need to be incorporated in such a model. In this paper, we use a semi-dynamic traffic assignment model that considers within-day dynamics by improving the static traffic assignment model. In addition, stochastic travel times are incorporated into the model. Thus, we propose a semi-dynamic traffic assignment model with mode choice between public transit and automobiles, route choice with stochastic travel times, and an accompanying computing algorithm. This model enables us to assess within-day dynamics of transportation networks and travel time reliability of public railways. Copyright © 2012 John Wiley & Sons, Ltd