Dense Molecular Gas Properties of the Central Kpc of Nearby Ultraluminous Infrared Galaxies Constrained by ALMA Three Transition-line Observations

We report the results of ALMA 1-2 kpc-resolution, three rotational transition line (J=2-1, J=3-2, and J=4-3) observations of multiple dense molecular gas tracers (HCN, HCO$^{+}$, and HNC) for ten nearby (ultra)luminous infrared galaxies ([U]LIRGs). Following the matching of beam sizes to 1-2 kpc for each (U)LIRG, the high-J to low-J transition-line flux ratios of each molecule and the emission line flux ratios of different molecules at each J transition are derived. We conduct RADEX non-LTE model calculations and find that, under a wide range of gas density and kinetic temperature, the observed HCN-to-HCO$^{+}$ flux ratios in the overall (U)LIRGs are naturally reproduced with enhanced HCN abundance compared to HCO$^{+}$. Thereafter, molecular gas properties are constrained primarily through the use of HCN and HCO$^{+}$ data and the adoption of fiducial values for the HCO$^{+}$ column density and HCN-to-HCO$^{+}$ abundance ratio. We quantitatively confirm the following: (1) Molecular gas at the (U)LIRGs' nuclei is dense ($\gtrsim$10$^{3-4}$ cm$^{-3}$) and warm ($\gtrsim$100 K). (2) Molecular gas density and temperature in nine ULIRGs' nuclei are significantly higher than that of one LIRG's nucleus. (3) Molecular gas in starburst-dominated sources tends to be less dense and cooler than ULIRGs with luminous AGN signatures. For six selected sources, we also apply a Bayesian approach by freeing all parameters and support the above main results. Our ALMA 1-2 kpc resolution, multiple transition-line data of multiple molecules are a very powerful tool for scrutinizing the properties of molecular gas concentrated around luminous energy sources in nearby (U)LIRGs' nuclei.Comment: 42 pages, 13 figures. ApJ accepte

ALMA 0.5 kpc Resolution Spatially Resolved Investigations of Nuclear Dense Molecular Gas Properties in Nearby Ultraluminous Infrared Galaxies Based on HCN and HCO+^{+} Three Transition Line Data

We present the results of our ALMA $\lesssim$0.5 kpc-resolution dense molecular line (HCN and HCO$^{+}$ J=2-1, J=3-2, and J=4-3) observations of 12 nearby (ultra)luminous infrared galaxies ([U]LIRGs). After matching beam sizes of all molecular line data to the same values in all (U)LIRGs, we derive molecular line flux ratios, by extracting spectra in the central 0.5, 1, 2 kpc circular regions, and 0.5-1 and 1-2 kpc annular regions. Based on non-LTE model calculations, we quantitatively confirm that the innermost ($\lesssim$0.5 kpc) molecular gas is very dense ($\gtrsim$10$^{5}$ cm$^{-3}$) and warm ($\gtrsim$300 K) in ULIRGs, and that in one LIRG is also modestly dense (10$^{4-5}$ cm$^{-3}$) and warm ($\sim$100 K). We then investigate the spatial variation of the HCN-to-HCO$^{+}$ flux ratios and high-J to low-J flux ratios of HCN and HCO$^{+}$. A subtle sign of decreasing trend of these ratios from the innermost ($\lesssim$0.5 kpc) to outer nuclear (0.5-2 kpc) region is discernible in a significant fraction of the observed ULIRGs. For two AGN-hosting ULIRGs which display the trend most clearly, we find based on a Bayesian approach that the HCN-to-HCO$^{+}$ abundance ratio and gas kinetic temperature systematically increase from the outer nuclear to the innermost region. We suggest that this trend comes from potential AGN effects, because no such spatial variation is found in a starburst-dominated LIRG.Comment: 62 pages, 29 figures. ApJ accepted. Higher resolution version is available at http://www2.nao.ac.jp/~masaimanishi/Aph/ImanishiULIRG500pcH.pd

The ontogeny of choanocyte chambers during metamorphosis in the demosponge Amphimedon queenslandica

Background The aquiferous body plan of poriferans revolves around internal chambers comprised of choanocytes, a cell type structurally similar to choanoflagellates. These choanocyte chambers perform a range of physiological and developmental functions, including the capture of food and the generation of stem cells. Despite the increasing interest for choanocytes as sponge stem cells, there is limited knowledge on the development of choanocyte chambers. Using a combination of cell lineage tracing, antibody staining and EdU labeling, here we examine the development of choanocytes and the chambers they comprise during metamorphosis in the marine demosponge Amphimedon queenslandica. Results Lineage-tracing experiments show that larval epithelial cells transform into mesenchymal pluripotent stem cells, resembling archeocytes, within 24 h of initiating metamorphosis. By 36 h, some of these labeled archeocyte-like cells have differentiated into choanocytes that will form the first postlarval choanocyte chambers. Non-labeled cells also contribute to these primary choanocyte chambers, consistent with these chambers being a chimera of multiple transdifferentiated larval cell types and not the proliferation of a single choanocyte precursor. Moreover, cell proliferation assays demonstrate that, following the initial formation of choanocyte chambers, chambers grow at least partially by the proliferation of choanocytes within the chamber, although recruitment of individual cells into established chambers also appears to occur. EdU labeling of postlarvae and juveniles reveals that choanocyte chambers are the primary location of cell proliferation during metamorphosis. Conclusion Our results show that multiple larval cell lineages typically contribute to formation of individual choanocyte chambers at metamorphosis, contrary to previous reports in other species that show sponge choanocyte chambers form clonally. Choanocytes in postlarval and juvenile A. queenslandica chambers can also divide, with choanocyte chambers being the primary location of cell proliferation. Interestingly, the level of cell proliferation varies greatly between chambers and appears to be contingent on the size, location and developmental state of the chamber. Small chambers on the periphery of the body tend to possess more dividing cells. As choanocytes can also dedifferentiate into archeocyte-like cells, cell proliferation in chambers may not only contribute to chamber growth and self-renewal but also increase the number of pluripotent archeocytes.Publisher PDFPeer reviewe

Evolutionary origin of gastrulation: insights from sponge development

BACKGROUND: The evolutionary origin of gastrulation—defined as a morphogenetic event that leads to the establishment of germ layers—remains a vexing question. Central to this debate is the evolutionary relationship between the cell layers of sponges (poriferans) and eumetazoan germ layers. Despite considerable attention, it remains unclear whether sponge cell layers undergo progressive fate determination akin to eumetazoan primary germ layer formation during gastrulation. RESULTS: Here we show by cell-labelling experiments in the demosponge Amphimedon queenslandica that the cell layers established during embryogenesis have no relationship to the cell layers of the juvenile. In addition, juvenile epithelial cells can transdifferentiate into a range of cell types and move between cell layers. Despite the apparent lack of cell layer and fate determination and stability in this sponge, the transcription factor GATA, a highly conserved eumetazoan endomesodermal marker, is expressed consistently in the inner layer of A. queenslandica larvae and juveniles. CONCLUSIONS: Our results are compatible with sponge cell layers not undergoing progressive fate determination and thus not being homologous to eumetazoan germ layers. Nonetheless, the expression of GATA in the sponge inner cell layer suggests a shared ancestry with the eumetazoan endomesoderm, and that the ancestral role of GATA in specifying internalised cells may antedate the origin of germ layers. Together, these results support germ layers and gastrulation evolving early in eumetazoan evolution from pre-existing developmental programs used for the simple patterning of cells in the first multicellular animals

Single-molecule photochemical reactions of Auger-ionized quantum dots

Photoinduced electron transfer in donor-acceptor systems composed of quantum dots (QDs) and electron donors or acceptors is a subject of considerable recent research interest due to the potential applications of such systems in both solar energy harvesting and degradation of organic pollutants. Herein, we employed single-molecule imaging and spectroscopy techniques for the detection of photochemical reactions between 1,4-diaminobutane (DAB) and CdSe/ZnS single QDs. We investigated the reactions by analyzing photoluminescence (PL) intensity and lifetime of QDs at ensemble and single-molecule levels. While DAB was applied to single QDs tethered on a cover slip or QDs dispersed in a solution, PL intensity of QD continuously decreased with a concomitant increase in the PL lifetime. Interestingly, these changes in the PL properties of QD were predominant under high-intensity photoactivation. We hypothesize that the above changes in the PL properties surface due to the transfer of an electron from DAB to Auger-ionized QD followed by elimination of a proton from DAB and the formation of a QD-DAB adduct. Thus, a continuous decrease in the PL intensity of QDs under high-intensity photoactivation is attributed to continuous photochemical reactions of DAB with single QDs and the formation of QD-(DAB)n adducts. We believe that detection and analysis of such photochemical reactions of single QDs with amines will be of considerable broad interest due to the significant impact of photoinduced electron transfer reactions in energy management and environmental remediation

Expression and localization of connective tissue growth factor (CTGF/Hcs24/CCN2) in osteoarthritic cartilage

AbstractObjectiveThe investigation of the expression and localization of connective tissue growth factor/hypertrophic chondrocyte-specific gene product 24/CCN family member 2 (CTGF/Hcs24/CCN2) in normal and osteoarthritic (OA) cartilage, and quantification of CTGF/Hcs24-positive cells.MethodsCartilage samples of patients (n=20) with late stage OA were obtained at total joint replacement surgery. Morphologically normal cartilage was harvested for comparison purposes from the femoral heads of 6 other patients with femoral neck fracture. Paraffin-embedded sections were stained by Safranin O. The severity of the OA lesions was divided into four stages (normal, early, moderate, and severe). The localization of protein and mRNA for CTGF/Hcs24 was investigated by immunohistochemistry and in situ hybridization, respectively. The population of CTGF/Hcs24-positive chondrocytes in OA cartilage and chondro-osteophyte was quantified by counting the number of the cells under light microscopy.ResultsSignals for CTGF/Hcs24 were detected in a small percentage of chondrocytes throughout the layers of normal cartilage. In early stage OA cartilage, the CTGF/Hcs24-positive chondrocytes were localized mainly in the superficial layer. In moderate to severe OA cartilage, intense staining for CTGF/Hcs24 was observed in proliferating chondrocytes forming cell clusters next to the cartilage surface. In chondro-osteophyte, strong signals were found in the chondrocytes of the proliferative and hypertrophic zones.ConclusionCTGF/Hcs24 expression was detected in both normal and OA chondrocytes of human samples. The results of the current study suggested that expression of CTGF/Hcs24 was concomitant with development of OA lesions and chondrocyte differentiation in chondro-osteophyte

Supermassive black hole feeding and feedback observed on sub-parsec scales

Active galaxies contain a supermassive black hole at their center, which grows by accreting matter from the surrounding galaxy. The accretion process in the central ~10 parsecs has not been directly resolved in previous observations, due to the small apparent angular sizes involved. We observed the active nucleus of the Circinus Galaxy using sub-millimeter interferometry. A dense inflow of molecular gas is evident on sub-parsec scales. We calculate that less than 3% of this inflow is accreted by the black hole, with the rest being ejected by multiphase outflows, providing feedback to the host galaxy. The observations also reveal a dense gas disk surrounding the inflow; the disk is gravitationally unstable which drives the accretion into the central ~1 parsec.Comment: First release on Nov 3, 2023 in Science. 32 pages in one column = Main (13 pages, 4 figures) + Supplement (19 pages, 9 figures + 2 tables). This is the accepted version after peer revie

Structural basis for PPARγ transactivation by endocrine-disrupting organotin compounds

Harada, S., Hiromori, Y., Nakamura, S. et al. Structural basis for PPARγ transactivation by endocrine-disrupting organotin compounds. Sci Rep 5, 8520 (2015). https://doi.org/10.1038/srep08520