3 research outputs found
Fast and Sensitive HPLC-ESI-MS/MS Method for Etoricoxib Quantification in Human Plasma and Application to Bioequivalence Study
Etoricoxib is a non-steroidal anti-inflammatory drug (NSAID) used to treat pain and inflammation. The objective of the current study was to develop a sensitive, fast and high-throughput HPLC-ESI-MS/MS method to measure etoricoxib levels in human plasma using a one-step methanol protein precipitation technique. A tandem mass spectrometer equipped with an electrospray ionization (ESI) source operated in a positive mode and multiple reaction monitoring (MRM) were used for data collection. The quantitative MRM transition ions were m/z 359.15 > 279.10 and m/z 363.10 > 282.10 for etoricoxib and IS. The linear range was from 10.00 to 4000.39 ng/mL and the validation parameters were within the acceptance limits of the European Medicine Agency (EMA) and Food and Drug Analysis (FDA) guidelines. The present method was sensitive (10.00 ng/mL with S/N > 40), simple, selective (K prime > 2), and fast (short run time of 2 min), with negligible matrix effect and consistent recovery, suitable for high throughput analysis. The method was used to quantitate etoricoxib plasma concentrations in a bioequivalence study of two 120 mg etoricoxib formulations. Incurred sample reanalysis results further supported that the method was robust and reproducible
Simple and rapid LC-MS/MS method for determination of sitagliptin in human plasma and application to bioequivalence study
Guideline for herbal medicine research
Herbal medicine is widely used in Traditional and
Complementary Medicine (T&CM) practices. With its
increasing use within the Malaysian community,
there needs to be more specific guidance on the
requirements for herbal medicine research to
produce evidence-based herbal medicine products
with therapeutic claims. Therefore, the pathway of
herbal product development involving preclinical and clinical research must be
well outlined in tandem with the ‘Guideline on Natural Products with Therapeutic
Claim’ from the regulators.
The primary purpose of this guideline is to provide structured guidance to be
utilised by researchers, academicians, clinicians, T&CM practitioners, and relevant
stakeholders of the herbal industry. This guideline outlines a concise yet
informative description of the prerequisites and processes involved in planning
and conducting herbal medicine-related research in the Malaysian context.
This guideline will bridge the gap often associated with the population's demand
for other treatment options besides conventional medicine. This effort will also
help accommodate the growing interest in herbal medicine research and spur the
discovery of herbal products with therapeutic claims in Malaysia