An Actuarial Analysis of Calibration of Crop Insurance Premiums to Heterogeneous Risks

This paper examines whether the loadings on the crop insurance premium rates for risks such as moral hazard and adverse selection are adequate. From the discrete choice (tobit) analysis conducted, we discover that the premium loadings for 75% coverage level are not adequate, resulting in losses for the Risk Management Agency

Bhopal Gas Tragedy – A Social, Economic, Legal and Environmental Analysis

The purpose of this paper is to examine the economic and legal aspects of the difficulties in obtaining compensation of chemical disaster that occurred India, when the parent company is located in the United States. After this disaster, several laws regulating chemical plant operation became mandatory in both US and in India

Bhopal Gas Tragedy – A Social, Economic, Legal and Environmental Analysis

The purpose of this paper is to examine the economic and legal aspects of the difficulties in obtaining compensation of chemical disaster that occurred India, when the parent company is located in the United States. After this disaster, several laws regulating chemical plant operation became mandatory in both US and in India

Arbitrage, Cointegration and Testing the Unbiasedness Hypothesis in Coffee Futures Traded at the CSCE

The objective of this study was to test the market efficiency hypothesis of Colombian coffee. This is of extreme importance to Colombia because the exports of coffee from this country provides for valuable foreign exchange and provides employment for her people. Historically this country has been concerned with the volatility of spot markets and used buffer stocks and quotas to protect her from price risk. The previous futures prices were found to be an unbiased predictor of current spot prices indicating the markets are efficient

An Actuarial Analysis of Calibration of Crop Insurance Premiums to Heterogeneous Risks

This paper examines whether the loadings on the crop insurance premium rates for risks such as moral hazard and adverse selection are adequate. From the discrete choice (tobit) analysis conducted, we discover that the premium loadings for 75% coverage level are not adequate, resulting in losses for the Risk Management Agency

Property rights orientations of landowners in Texas, Utah and Colorado

The debate over allocation of rangeland resources has gained increasing momentum in the 1990s. These days, several constraints are facing landowners, including high estate taxes, reduced profit margins of agricultural/ranching operations and increased legal restrictions in land use. Previous studies point out to strong private property rights among landowners, which have often been assumed to lead to short-term land management goals that are not particularly beneficial to society. This study analyses the multidimensionality of property rights and how this determines the variation in willingness to undertake various ecologically sustainable management practices without compensation and the variation in the perception of threats by the landowner. A study was conducted on randomly selected landowners in three states, Texas, Utah and Colorado in 2001; an average response rate of 51.3% was obtained across all three states. A descriptive analysis was conducted, tabulating the identifying characteristics of the respondent rancher/farmer and their property, their opinion regarding the rights and responsibilities of landowner, their likely willingness to implement different management practices and threats to the future viability of their ranching operation, searching for testable hypotheses. In analysis of effect of multidimensionality of property rights on the willingness to undertake management practices without compensation, results confirmed the significance of three property rights except the individual property rights scale. Respondents perception of the threats to the future viability of future operation was analyzed using directed acyclic graphs (DAG). The DAG revealed several directed edges (causal effects), but the presence of several bi-directed edges (cause and effect being indeterminable) were also identified. The subsequent regression analysis showed no significant property rights scales, but component analyses identified a few significant property rights orientations. The low significance is attributed to the presence of bi-directed edges

The Two-Domain LysX Protein of Mycobacterium tuberculosis Is Required for Production of Lysinylated Phosphatidylglycerol and Resistance to Cationic Antimicrobial Peptides

The well-recognized phospholipids (PLs) of Mycobacterium tuberculosis (Mtb) include several acidic species such as phosphatidylglycerol (PG), cardiolipin, phosphatidylinositol and its mannoside derivatives, in addition to a single basic species, phosphatidylethanolamine. Here we demonstrate that an additional basic PL, lysinylated PG (L-PG), is a component of the PLs of Mtb H37Rv and that the lysX gene encoding the two-domain lysyl-transferase (mprF)-lysyl-tRNA synthetase (lysU) protein is responsible for L-PG production. The Mtb lysX mutant is sensitive to cationic antibiotics and peptides, shows increased association with lysosome-associated membrane protein–positive vesicles, and it exhibits altered membrane potential compared to wild type. A lysX complementing strain expressing the intact lysX gene, but not one expressing mprF alone, restored the production of L-PG and rescued the lysX mutant phenotypes, indicating that the expression of both proteins is required for LysX function. The lysX mutant also showed defective growth in mouse and guinea pig lungs and showed reduced pathology relative to wild type, indicating that LysX activity is required for full virulence. Together, our results suggest that LysX-mediated production of L-PG is necessary for the maintenance of optimal membrane integrity and for survival of the pathogen upon infection

31st Annual Meeting and Associated Programs of the Society for Immunotherapy of Cancer (SITC 2016) : part two

Background The immunological escape of tumors represents one of the main ob- stacles to the treatment of malignancies. The blockade of PD-1 or CTLA-4 receptors represented a milestone in the history of immunotherapy. However, immune checkpoint inhibitors seem to be effective in specific cohorts of patients. It has been proposed that their efficacy relies on the presence of an immunological response. Thus, we hypothesized that disruption of the PD-L1/PD-1 axis would synergize with our oncolytic vaccine platform PeptiCRAd. Methods We used murine B16OVA in vivo tumor models and flow cytometry analysis to investigate the immunological background. Results First, we found that high-burden B16OVA tumors were refractory to combination immunotherapy. However, with a more aggressive schedule, tumors with a lower burden were more susceptible to the combination of PeptiCRAd and PD-L1 blockade. The therapy signifi- cantly increased the median survival of mice (Fig. 7). Interestingly, the reduced growth of contralaterally injected B16F10 cells sug- gested the presence of a long lasting immunological memory also against non-targeted antigens. Concerning the functional state of tumor infiltrating lymphocytes (TILs), we found that all the immune therapies would enhance the percentage of activated (PD-1pos TIM- 3neg) T lymphocytes and reduce the amount of exhausted (PD-1pos TIM-3pos) cells compared to placebo. As expected, we found that PeptiCRAd monotherapy could increase the number of antigen spe- cific CD8+ T cells compared to other treatments. However, only the combination with PD-L1 blockade could significantly increase the ra- tio between activated and exhausted pentamer positive cells (p= 0.0058), suggesting that by disrupting the PD-1/PD-L1 axis we could decrease the amount of dysfunctional antigen specific T cells. We ob- served that the anatomical location deeply influenced the state of CD4+ and CD8+ T lymphocytes. In fact, TIM-3 expression was in- creased by 2 fold on TILs compared to splenic and lymphoid T cells. In the CD8+ compartment, the expression of PD-1 on the surface seemed to be restricted to the tumor micro-environment, while CD4 + T cells had a high expression of PD-1 also in lymphoid organs. Interestingly, we found that the levels of PD-1 were significantly higher on CD8+ T cells than on CD4+ T cells into the tumor micro- environment (p < 0.0001). Conclusions In conclusion, we demonstrated that the efficacy of immune check- point inhibitors might be strongly enhanced by their combination with cancer vaccines. PeptiCRAd was able to increase the number of antigen-specific T cells and PD-L1 blockade prevented their exhaus- tion, resulting in long-lasting immunological memory and increased median survival