Experimental validation of phase space conduits of transition between potential wells

A phase space boundary between transition and non-transition, similar to those observed in chemical reaction dynamics, is shown experimentally in a macroscopic system. We present a validation of the phase space flux across rank one saddles connecting adjacent potential wells and confirm the underlying phase space conduits that mediate the transition. Experimental regions of transition are found to agree with the theory to within 1\%, suggesting the robustness of phase space conduits of transition in a broad array of two or more degree of freedom experimental systems, despite the presence of small dissipation.Comment: 7 pages, 6 figure

Noise thermometry and electron thermometry of a sample-on-cantilever system below 1 Kelvin

We have used two types of thermometry to study thermal fluctuations in a microcantilever-based system below 1 K. We measured the temperature of a cantilever's macroscopic degree-of-freedom (via the Brownian motion of its lowest flexural mode) and its microscopic degrees-of-freedom (via the electron temperature of a metal sample mounted on the cantilever). We also measured both temperatures' response to a localized heat source. We find it possible to maintain thermal equilibrium between these two temperatures and a refrigerator down to at least 300 mK. These results are promising for ongoing experiments to probe quantum effects using micromechanical devices

On abstraction refinement for program analyses in Datalog

A central task for a program analysis concerns how to efficiently find a program abstraction that keeps only information relevant for proving properties of interest. We present a new approach for finding such abstractions for program analyses written in Datalog. Our approach is based on counterexample-guided abstraction refinement: when a Datalog analysis run fails using an abstraction, it seeks to generalize the cause of the failure to other abstractions, and pick a new abstraction that avoids a similar failure. Our solution uses a boolean satisfiability formulation that is general, complete, and optimal: it is independent of the Datalog solver, it generalizes the failure of an abstraction to as many other abstractions as possible, and it identifies the cheapest refined abstraction to try next. We show the performance of our approach on a pointer analysis and a typestate analysis, on eight real-world Java benchmark programs

Poly(D,L-lactide-co-glycolide) microcomposite containing magnetic iron core nanoparticles as a drug carrier

Today many potent anticancer drugs like cisplatin are available which carry a number of side effects. A promising way of reducing the side effects is to target the drug to tissue sites by coating it with biocompatible materials like Poly (dl-lactide-co-glycolide) (PLGA) polymer where controlled drug release is achieved during the biodegradation of the polymer. Also the efficacy of anticancer drugs like cisplatin increases at elevated temperatures, so if local heating can be achieved where the drug is targeted. Local heating can be achieved by introducing iron core nanoparticles in the composites along with the drug, which can be heated by the 2.4 GHz microwaves. Local heating of the nanocomposites also helps to swell the polymer shell and enhance the drug release. The magnetic nanocomposites were synthesized using ironnanoparticles, PLGA and a fluorescent dye, tris-(2,2′bipyridyl) dichlororuthenium (II) using an oil-in-emulsion technique. The emulsion contains PLGA, dye, and ironnanoparticles dissolved in the oil phase and polyvinyl alcohol (PVA) as a stabilizer. As the sample is homogenized, and dried, uniform 100 nm composites are formed where the dye and ironnanoparticles are encapsulated in a PLGA shell. Control of the thickness and loading efficiency of the nanocomposite can be controlled by varying the ratio of PLGA, iron, and dye. The amount of loading was determined using TGA confirming from 20–50% (w/w) loading. As the dye is released from the composite the fluorescence intensity decreases due to self-quenching. This self-quenching allows for the determination of the release kinetics as a function of temperature using fluorescence spectroscopy. Initial results suggest that there is a release of 5–10% of the dye from the composite at 25°C and complete release after the nanocomposite reaches 90°C. Using local microwave heating the complete release of the dye can be accomplished with three two second pulses of 2.4 GHz microwaves. This allows for the complete drug delivery platform which allows for the controlled release using microwave frequency

Fast Transition between High-soft and Low-soft States in GRS 1915+105: Evidence for a Critically Viscous Accretion Flow

We present the results of a detailed analysis of RXTE observations of class $\omega$ which show an unusual state transition between high-soft and low-soft states in the microquasar GRS 1915+105. Out of about 600 pointed RXTE observations, the source was found to exhibit such state transition only on 16 occasions. An examination of the RXTE/ASM data in conjunction with the pointed observations reveals that these events appeared as a series of quasi-regular dips in two stretches of long duration (about 20 days during each occasions) when hard X-ray and radio flux were very low. The X-ray light curve and color-color diagram of the source during these observations are found to be different from any reported so far. The duration of these dips is found to be of the order of a few tens of seconds with a repetition time of a few hundred seconds. The transition between these dips and non-dips which differ in intensity by a factor of ~ 3.5, is observed to be very fast (~ a few seconds). It is observed that the low-frequency narrow QPOs are absent in the power density spectrum (PDS) of the dip and non-dip regions of class $\omega$ and the PDS is a power law in 0.1 - 10 Hz frequency range. There is a remarkable similarity in the spectral and timing properties of the source during the dip and non-dip regions in these set of observations. These properties of the source are distinctly different from those seen in the observations of other classes. This indicates that the basic accretion disk structure during both dip and non-dip regions of class $\omega$ is similar, but differ only in intensity. To explain these observations, we invoke a model in which the viscosity is very close to critical viscosity and the shock wave is weak or absent.Comment: Replaced with correct figures, Jour. of Astrophysics and Astronomy (accepted

GRK6 regulates the hemostatic response to injury through its rate-limiting effects on GPCR signaling in platelets.

G protein-coupled receptors (GPCRs) mediate the majority of platelet activation in response to agonists. However, questions remain regarding the mechanisms that provide negative feedback toward activated GPCRs to limit platelet activation and thrombus formation. Here we provide the first evidence that GPCR kinase 6 (GRK6) serves this role in platelets, using GRK6-/- mice generated by CRISPR-Cas9 genome editing to examine the consequences of GRK6 knockout on GPCR-dependent signaling. Hemostatic thrombi formed in GRK6-/- mice are larger than in wild-type (WT) controls during the early stages of thrombus formation, with a rapid increase in platelet accumulation at the site of injury. GRK6-/- platelets have increased platelet activation, but in an agonist-selective manner. Responses to PAR4 agonist or adenosine 5\u27-diphosphate stimulation in GRK6-/- platelets are increased compared with WT littermates, whereas the response to thromboxane A2 (TxA2) is normal. Underlying these changes in GRK6-/- platelets is an increase in Ca2+ mobilization, Akt activation, and granule secretion. Furthermore, deletion of GRK6 in human MEG-01 cells causes an increase in Ca2+ response and PAR1 surface expression in response to thrombin. Finally, we show that human platelet activation in response to thrombin causes an increase in binding of GRK6 to PAR1, as well as an increase in the phosphorylation of PAR1. Deletion of GRK6 in MEG-01 cells causes a decrease in PAR1 phosphorylation. Taken together, these data show that GRK6 regulates the hemostatic response to injury through PAR- and P2Y12-mediated effects, helping to limit the rate of platelet activation during thrombus growth and prevent inappropriate platelet activation

Comparative study of stripe magnetic domains in epitaxial Ni(111) and Co(0001) films

The evolution of stripe magnetic domain structures observed by magnetic force microscopy on epitaxial Ni(111) and Co(0001) films as a function of film thickness is successfully explained by a periodic one-dimensional model with tilted partial flux closure domains. The model predicts a sizable fraction of the magnetization not being parallel to the film’s normal, which consequentially results in an in-plane magnetization in agreement with the experimentally observed magnetization for these films. © 2002 American Institute of Physics.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/69860/2/JAPIAU-91-10-7550-1.pd

Rapid, multiplexed microfluidic phage display

The development of a method for high-throughput, automated proteomic screening could impact areas ranging from fundamental molecular interactions to the discovery of novel disease markers and therapeutic targets. Surface display techniques allow for efficient handling of large molecular libraries in small volumes. In particular, phage display has emerged as a powerful technology for selecting peptides and proteins with enhanced, target-specific binding affinities. Yet, the process becomes cumbersome and time-consuming when multiple targets are involved.Here we demonstrate for the first time a microfluidic chip capable of identifying high affinity phage displayed peptides for multiple targets in just a single round and without the need for bacterial infection. The chip is shown to be able to yield well-established control consensus sequences while simultaneously identifying new sequences for clinically important targets. Indeed, the confined parameters of the device allow not only for highly controlled assay conditions but also introduce a significant time-reduction to the phage display process. We anticipate that this easily-fabricated, disposable device has the potential to impact areas ranging from fundamental studies of protein, peptide, and molecular interactions, to applications such as fully automated proteomic screening

B_K from improved staggered quarks

We compare calculations of B_K with improved staggered quarks (HYP, Asqtad) and demonstrate the improved scaling behaviour that this gives rise to over previous calculations with unimproved staggered quarks. This enables us to perform the calculation of B_K on the MILC dynamical configurations (n_f=2+1), for which we give preliminary results.Comment: 3 pages, 3 figures. Talk presented at Lattice 2004(weak), Fermilab, June 21-26, 200