A new distributed storage evaluating with irrefutable outsourcing of key upgrades

In this worldview, key updates can be securely redistributed to some approved gathering, and along these lines the key-refresh trouble on the customer will be kept negligible. In particular, we use the third party auditor (TPA) in many existing open evaluating structures, let it assume the job of approved gathering for our situation, and make it accountable for both the capacity reviewing and the protected key updates for key-introduction obstruction. In our structure, TPA just needs to hold a scrambled variant of the customer's secret key, while doing all these oppressive errands in the interest of the customer. The customer just needs to download the scrambled secret key from the TPA while transferring new documents to cloud. Plus, our structure additionally outfits the customer with ability to additionally confirm the legitimacy of the scrambled secret keys given by TPA

Gene Expression Signature of DMBA-Induced Hamster Buccal Pouch Carcinomas: Modulation by Chlorophyllin and Ellagic Acid

Chlorophyllin (CHL), a water-soluble, semi-synthetic derivative of chlorophyll and ellagic acid (EA), a naturally occurring polyphenolic compound in berries, grapes, and nuts have been reported to exert anticancer effects in various human cancer cell lines and in animal tumour models. The present study was undertaken to examine the mechanism underlying chemoprevention and changes in gene expression pattern induced by dietary supplementation of chlorophyllin and ellagic acid in the 7,12-dimethylbenz[a]anthracene (DMBA)-induced hamster buccal pouch (HBP) carcinogenesis model by whole genome profiling using pangenomic microarrays. In hamsters painted with DMBA, the expression of 1,700 genes was found to be altered significantly relative to control. Dietary supplementation of chlorophyllin and ellagic acid modulated the expression profiles of 104 and 37 genes respectively. Microarray analysis also revealed changes in the expression of TGFβ receptors, NF-κB, cyclin D1, and matrix metalloproteinases (MMPs) that may play a crucial role in the transformation of the normal buccal pouch to a malignant phenotype. This gene expression signature was altered on treatment with chlorophyllin and ellagic acid. Our study has also revealed patterns of gene expression signature specific for chlorophyllin and ellagic acid exposure. Thus dietary chlorophyllin and ellagic acid that can reverse gene expression signature associated with carcinogenesis are novel candidates for cancer prevention and therapy

Functional correlation of cyclooxygenases-l, 2 and 3 from amino acid sequences and three dimensional model structures

182-187COX-1, COX-2 and COX-3, three isoforms of cyclooxygenase are differentially expressed . We have analyzed the sequences of these cyclooxygenases and built the three dimensional model structures for human COX-1, COX-2 and canine COX-3 to characterize the function of cyclooxygenase isozymes based on the sequence and structure information. Sequence analysis reveals that COX-3 shares 90% homology with COX-1 and 60% with COX-2. The COX-1 model has been compared with those of COX-2 and COX-3 and the active site regions have been analyzed. The major differences in the active sites of COX-1 and COX-2 are: Ile 523 in COX-1 is replaced by Val in COX-2, apart from a few mutations at the mouth of the active site. No such differences in the active sites are seen between COX-1 and COX-3 structures and the amino acid residues that differ between COX-1 and COX-3 lie only on the surface of the protein. Therefore, it is hard to explain the specificity of acetaminophen towards COX-3. Further, the nitron 1 of canine COX-1 gene that is retained in COX-3 mRNA transcript codes for a poly proline region that could be responsible for intermolecular interactions