1 research outputs found
Comparative Glycomics of Fat Globule Membrane Glycoconjugates from Buffalo (<i>Bubalus bubalis</i>) Milk and Colostrum
The health-promoting effects of milk
fat globule membrane (MFGM)
glycoconjugates has attracted curiosity especially with regard to
the challenges encountered to unravel the glycan complexities of MFGM
glycoproteins and glycosphingolipids. In this context, we characterized
glycans present in buffalo milk and colostrum fat globule membranes
by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF)
MS analysis by adopting chemoselective glycoblotting technique. Unlike
human and bovine MFGM glycoproteins, the variations were obvious with
respect to their number, size, heterogeneity, and abundance among
the samples analyzed. Among N-linked glycans, mono-, di-, and trisialyl
glycans were apparent in colostrum, while MFGM predominantly
contained mono- and disialyl glycans, in addition to neutral and high-mannose
glycoforms. The structural assignments of major glycans were confirmed
by TOF/TOF analysis. Core 1 O-glycans were more common in both samples,
and the major glycosphingolipids were GM3 and GD3 irrespective of
the samples analyzed. The colostrum N-glycans, being effective antibacterials
against human pathogens, established the structure–function
relationship of oligosaccharides in early milk in providing innate
protection to the newborn