Relationship between radiographic grading of osteoarthritis and the biochemical markers for arthritis in knee osteoarthritis

The aim of this study was to investigate the relationship between the biochemical markers of arthritis and the radiographic grading of osteoarthritis (OA) in knees. Seventy-one women aged 49–85 years with knee OA were studied. Anterior–posterior knee radiographs and hand radiographs were taken in all patients. The radiographic grading of OA in the knee was performed by using the Kellgren–Lawrence criteria and the joint space width. The 71 patients with knee OA were divided into two groups: 37 patients exhibiting generalized osteoarthritis (GOA) and 34 non-GOA patients, according to the grading of their hand radiograph. C-reactive protein (CRP), urinary pyridinoline, YKL-40, plasma matrix metalloproteinase (MMP)-3, MMP-9 and tissue inhibitor of metalloproteinases (TIMP)-1 were measured as the biochemical markers of arthritis. The radiographic grading with the Kellgren–Lawrence scale revealed a significant relationship to the joint space width (P = 0.003): the joint space width decreased with increasing Kellgren–Lawrence grade. All biochemical markers had negative correlations with the joint space width, but only urinary pyridinoline had a significant correlation (P = 0.039). Pyridinoline (P = 0.034) and TIMP-1 (P = 0.017) also exhibited a significant relationship to the Kellgren–Lawrence grade. In GOA evaluations, the joint space width did not differ between GOA and non-GOA patients. CRP, pyridinoline, YKL-40 and MMP-3 levels were significantly greater in GOA patients than in non-GOA patients. CRP, pyridinoline, YKL-40, MMP-3 and TIMP-1 levels each related to at least one of the radiographic gradings. Furthermore, pyridinoline related to every type of radiographic grading examined in the present study

Hepatocyte growth factor prevents intimal hyperplasia in rabbit carotid expanded polytetrafluoroethylene grafting

AbstractPurpose: The major cause of vascular prosthesis failure is anastomotic intimal hyperplasia caused by the proliferation and migration of smooth muscle cells. Hepatocyte growth factor (HGF) is an endothelium-specific growth factor that exerts a mitogenic action on endothelial cells. This study was designed to examine the effect of HGF on the suppression of intimal hyperplasia after small-caliber expanded polytetrafluoroethylene (ePTFE) grafting. Methods: An ePTFE graft, 2 mm in diameter and 30 mm in length, was implanted in the left common carotid arteries of Japanese white rabbits, after which the animals were fed with a 1.0% cholesterol diet. HGF was infused intravenously immediately and then every day for 7 days at doses of 0.3 mg/body (the 0.3-mg HGF group; n = 20) or 1.0 mg/body (the 1.0-mg HGF group; n = 17). A control group (n = 20) underwent infusion with saline solution. The rabbits were killed on postoperative days (PODs) 1, 2, 3, 5, 7, and 28. Results: The patency rates on POD 28 were 33%, 55%, and 100% in the control, the 0.3-mg HGF, and the 1.0-mg HGF groups, respectively, with a significant difference between the control and the 1.0-mg HGF group (P <.05). Endothelial-like cells were seen on the intraluminal surface of the graft only near the anastomotic site on POD 5 in the 1.0-mg HGF group. Intimal thickness at the distal anastomosis was 284 ± 140 μm, 106 ± 18 μm, and 67 ± 10 μm in the control, the 0.3-mg HGF, and the 1.0-mg HGF groups, respectively, with a significant difference between the control and both HGF groups (P <.05). The number of anti-embryonic smooth muscle antibody positive cells at the distal anastomosis was 28.6 ± 0.8, 3.8 ± 2.8, and 3.9 ± 0.9 in the control, the 0.3-mg HGF, and the 1.0-mg HGF groups, respectively, with a significant difference between the control and both HGF groups (P <.01). Conclusion: HGF might suppress intimal thickness at the anastomotic site and improve the patency rate via rapid reendothelialization by POD 28 in a rabbit carotid ePTFE grafting model. (J Vasc Surg 2002;35:786-91.

キュウセイ ジンフゼン ドウブツ モデル ニ タイスル カン サイボウ ゾウショク インシ ノ ユウコウセイ

京都大学0048新制・論文博士博士(農学)乙第11351号論農博第2507号新制||農||884(附属図書館)学位論文||H15||N3873(農学部図書室)UT51-2003-U512(主査)教授 伏木 亨, 教授 井上 國世, 教授 熊谷 英彦学位規則第4条第2項該当Doctor of Agricultural ScienceKyoto UniversityDA

キュウセイ ジンフゼン ドウブツ モデル ニ タイスル カン サイボウ ゾウショク インシ ノ ユウコウセイ

京都大学0048新制・論文博士博士(農学)乙第11351号論農博第2507号新制||農||884(附属図書館)学位論文||H15||N3873(農学部図書室)UT51-2003-U512(主査)教授 伏木 亨, 教授 井上 國世, 教授 熊谷 英彦学位規則第4条第2項該当Doctor of Agricultural ScienceKyoto UniversityDA

The Effect of Hepatocyte Growth Factor (HGF) on Promotion of Endothelial Healing of Balloon-injured Carotid Artery

Purpose: This animal study was designed to examine the effect of hepatocyte grouth factor on suppression of intimal hyperplasia resulting from reendothelialization after balloon injury in the rat carotid artery. Methods: Fourteen Sprague-Dawely rats were subjected to balloon injur

Relationship between Kellgren–Lawrence grading (KL), joint space width (JSW) and biochemical markers

<p><b>Copyright information:</b></p><p>Taken from "Relationship between radiographic grading of osteoarthritis and the biochemical markers for arthritis in knee osteoarthritis"</p><p>Arthritis Research & Therapy 2004;6(3):R208-R212.</p><p>Published online 12 Mar 2004</p><p>PMCID:PMC416443.</p><p>Copyright © 2004 Takahashi et al., licensee BioMed Central Ltd. This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original URL.</p> Pyridinoline (Pyr) level is expressed as nmol/mmol creatinine. There were significant relationships between joint space width (= 0.003), pyridinoline (= 0.034) and TIMP-1 (= 0.017) determined with the Kruskal–Wallis test. Bars indicate maximum and minimum values within the observation range. values are shown in each panel; N.S. indicates not significant. CRP, C-reactive protein; MMP, matrix metalloproteinase; TIMP, tissue inhibitor of metalloproteinases