Crystal structure of (E)-1,2-diferrocenyl-1,2-bis­(furan-2-yl)ethene

The title compound, [Fe₂(C₅H₅)₂(C₂₀H₁₄O₂)], is the product of a new synthetic route towards tetraaryl/hetaryl-substituted ethenes that reduces the occurrence of side-products. In the crystal, the molecule is centrosymmetric and the cyclopentadienyl (Cp) rings are nearly coplanar and aligned slightly closer to a staggered conformation than to an eclipsed one. The ethene plane is tilted by 32.40 (18)° with respect to that of the substituted Cp ring and by 63.19 (19)° with respect to that of the furan ring. C—H··· π interactions link the molecules into a three-dimensional supramolecular framework.Funding for this research was provided by: Maestro-3 (grant No. Dec-2012/06/A/ST5/00219 to R. Hamera-Fałdyga, G. Mlostoń); Institutspartnerschaft, Alexander von Humboldt Foundation, Bonn (grant to R. Hamera-Fałdyga, G. Mlostoń)

ジホスフェン カゴウブツ ノ トクセイ オ イカシタ シンキ パイ キョウヤク ブッシツ ノ コウチク ニ カンスル ケンキュウ

京都大学0048新制・論文博士博士(理学)乙第12181号論理博第1498号新制||理||1488(附属図書館)UT51-2008-C951岩手大学大学院工学研究科物質工学専攻(主査)教授 時任 宣博, 教授 林 民生, 教授 大須賀 篤弘学位規則第4条第2項該当Doctor of ScienceKyoto UniversityDA

SsODN-mediated knock-in with CRISPR-Cas for large genomic regions in zygotes

The CRISPR-Cas system is a powerful tool for generating genetically modified animals; however, targeted knock-in (KI) via homologous recombination remains difficult in zygotes. Here we show efficient gene KI in rats by combining CRISPR-Cas with single-stranded oligodeoxynucleotides (ssODNs). First, a 1-kb ssODN co-injected with guide RNA (gRNA) and Cas9 messenger RNA produce GFP-KI at the rat Thy1 locus. Then, two gRNAs with two 80-bp ssODNs direct efficient integration of a 5.5-kb CAG-GFP vector into the Rosa26 locus via ssODN-mediated end joining. This protocol also achieves KI of a 200-kb BAC containing the human SIRPA locus, concomitantly knocking out the rat Sirpa gene. Finally, three gRNAs and two ssODNs replace 58-kb of the rat Cyp2d cluster with a 6.2-kb human CYP2D6 gene. These ssODN-mediated KI protocols can be applied to any target site with any donor vector without the need to construct homology arms, thus simplifying genome engineering in living organisms