Adaptive approach formation using machine vision technology to determine the parameters of enrichment products deposition

In this paper, an adaptive approach has been developed for automatic initialization of the thickening curve using machine vision technology, which makes it possible to determine with high accuracy the material parameters necessary for the design of thickening and clarification apparatuses. Software has been developed that made it possible to search for the coordinates of the condensation critical point in automatic mode. Studies on two samples of materials (tailings of apatite-containing ores and gold-bearing concentrate) were carried out and made it possible to statistically prove the reproducibility of the results obtained using the parametric criteria of Fisher and Bartlett. It has been established that the deposition curves are approximated with high accuracy by the Weibull model, which, together with the piecewise linear approximation, makes it possible to formalize the method for determining the critical point coordinates. The empirical coefficients of the Weibull model for two samples are found, and the final liquefaction and settling rates of the studied materials are determined

Features of Ultrafine Fibers Made by Electrostatic Formation from Poly(3-Hydroxybutyrate)

Представлены результаты получения и исследования ультратонких волокон (УВ), сформированных из резорбируемого поли-3-гидроксибутирата (П3ГБ) методом электростатического формования (ЭСФ). Исследовано влияние плотности полимерных растворов на диаметр волокон и физико-механические свойства нетканого полотна (НП), сформированного УВ. Показано, что при увеличении концентрации раствора от 2 до 8 % повышается эластичность сформованных изделий (удлинение при разрыве возрастает от 4,5 до 10,6 %) при одновременном снижении их прочности (модуль Юнга снижается с 1,16 до 0,3 ГПа). На примере культуры фибробластов линии NIH 3T3 по результатам окрашивания клеток флуоресцентным фондом на ДНК DAPI и МТТ теста полученные образцы УВ положительно оценены в качестве опорных носителей для культивирования клеток.Results of construction and investigation of ultrafine fibers (UFF) made of resorbable poly(3 hydroxybutyrate) by electrostatic formation are presented in this study. Influence of concentration of polymer solutions on fiber diameters and physical-mechanical properties of nonwoven fabric (NF) was investigated. While concentration of polymer solutions increased from 2 to 8 % elongation at break of NF increased from 4,5 to 10,6 % and Young’s modulus decreased from 1,16 to 0,3 GPa. Murine fibroblasts NIH 3T3 were seeded on NF. Using DAPI staining and MTT assay UFF samples were estimated as good cell supporting carriers

Changes in the Differentiation Program of Birch Cambial Derivatives following Trunk Girdling

The mechanisms regulating the tree trunk radial growth can be studied in original experiments. One technique for studying cambium activity (the meristem involved in radial growth) under conditions of an increased photoassimilate level is trunk girdling. We girdled the trunks of 17- to 22-year-old silver birch plants (Betula pendula Roth var. pendula) during the active growth period and collected xylem and phloem samples at two height levels (1 cm and 35 cm) above girdle, 10, 20, and 30 days after girdling. We investigated the changes that occurred at the anatomical level, as well as the activities of sucrose-metabolizing enzymes and antioxidant-system enzymes and the expression of genes that encode proteins involved in sucrose and auxin transport and metabolism. A moderate increase in photoassimilates (35 cm above the girdle) resulted in a change in the ratio of phloem to xylem increments and an increase in the proportion of parenchyma in the conducting tissues. The increase of photoassimilates above the level at which they can be used in the processes of normal tissue growth and development (1 cm above the girdle) led to xylogenesis suppression and the stimulation of phloem formation, a significant increase in the parenchyma proportion in the conducting tissues, and formation of large sclereid complexes. The differentiation of parenchyma and sclereid cells coincided with biochemical and molecular markers of abnormal conducting tissue formation in Karelian birch, which are also characterized by high proportions of parenchyma and sclereid near the cambium. The results obtained are important in understanding the cambium responses to the photoassimilate distribution changes and estimating tree productivity and survival under changing environmental conditions

Features of Ultrafine Fibers Made by Electrostatic Formation from Poly(3-Hydroxybutyrate)

Представлены результаты получения и исследования ультратонких волокон (УВ), сформированных из резорбируемого поли-3-гидроксибутирата (П3ГБ) методом электростатического формования (ЭСФ). Исследовано влияние плотности полимерных растворов на диаметр волокон и физико-механические свойства нетканого полотна (НП), сформированного УВ. Показано, что при увеличении концентрации раствора от 2 до 8 % повышается эластичность сформованных изделий (удлинение при разрыве возрастает от 4,5 до 10,6 %) при одновременном снижении их прочности (модуль Юнга снижается с 1,16 до 0,3 ГПа). На примере культуры фибробластов линии NIH 3T3 по результатам окрашивания клеток флуоресцентным фондом на ДНК DAPI и МТТ теста полученные образцы УВ положительно оценены в качестве опорных носителей для культивирования клеток.Results of construction and investigation of ultrafine fibers (UFF) made of resorbable poly(3 hydroxybutyrate) by electrostatic formation are presented in this study. Influence of concentration of polymer solutions on fiber diameters and physical-mechanical properties of nonwoven fabric (NF) was investigated. While concentration of polymer solutions increased from 2 to 8 % elongation at break of NF increased from 4,5 to 10,6 % and Young’s modulus decreased from 1,16 to 0,3 GPa. Murine fibroblasts NIH 3T3 were seeded on NF. Using DAPI staining and MTT assay UFF samples were estimated as good cell supporting carriers