<i>F. vesca</i> PIP isoform-specific and reference gene primers.

<p><i>F. vesca</i> PIP isoform-specific and reference gene primers.</p

Diurnal expression of <i>F. vesca PIP</i> aquaporins.

<p>Diurnal expression pattern of <i>FvPIP</i> genes in leaves of <i>F. vesca</i> on two consecutive days. Grey columns – control plants, black columns – drought-stressed plants. Data are means+SE, <i>n</i> = 3 biological replicates. Different letters annotate statistically significant differences determined by LSD following repeated measurements ANOVA (<i>P</i><0.05). The difference between treatments was significant for (a), (b), (c), and (d).</p

Deduced protein sequences of <i>F. vesca</i> PIP aquaporins.

<p>An alignment of <i>F. vesca</i> PIP deduced protein sequences. Blue – transmembrane domains (TM); red – NPA motif; green highlight – residues of the aromatic/arginine selectivity filter involved in determining water specificity. Asterisk denotes conserved sites. N stands for amino-terminal region and C stands for carboxy-terminal region of the protein.</p

Relative <i>PIP</i> expression in leaves and roots of <i>F. vesca</i> under different levels of drought stress.

<p>Expression of four <i>F. vesca PIP</i> aquaporin genes after six days of drought stress (a, c, e, g) and upon re-watering (b, d, f, h). C – control plants; D25– plants receiving 25% of the control irrigation; D0 − plants with no irrigation. Data are means+SE, <i>n</i> = 4 plants. Different letters denote statistically significant differences within each time point determined by LSD following one way ANOVA (<i>P</i><0.05).</p

Correlation between substrate moisture content and relative expression of <i>FvPIP</i> genes.

<p><i>FvPIP2;1</i> (a, b), <i>FvPIP2;2</i> (c, d), <i>FvPIP1;1</i> (e,f), <i>FvPIP1;2</i> (g, h) in leaves (a, c, e, g) and roots (b, d, f, h). Regression lines, correlation coefficients and probabilities are given for statistically significant relationships.</p

The number of SNP markers derived from both <i>Malus</i> and <i>Pyrus</i> genomic sequence mapped in this investigation displaying evidence for probe sequence variants, null alleles and non-specific probe binding.

<p>The number of SNP markers derived from both <i>Malus</i> and <i>Pyrus</i> genomic sequence mapped in this investigation displaying evidence for probe sequence variants, null alleles and non-specific probe binding.</p

Summary of SNP markers reclassified through manual annotation.

<p>Reclassification of the 921 probes manually annotated in this investigation into probes segregating in two or three clusters with divergent probe annealing sites (<i>n</i> = 202) or annealing to paralogous loci (<i>n</i> = 616), and those revealing four segregating clusters (<i>n</i> = 91), or more than four clusters (<i>n</i> = 33).</p

A comparison of genetic and physical positions of probes mapped in M432.

<p>A comparison of the percentage of probes mapped to each linkage group on the M432 linkage map with their physical positions on the ‘Golden Delicious’ genome sequence.</p

Comparison of probe annealing numbers, behaviour and probe GenTrain scores.

<p>(a) The number of probes mapped in the M432 progeny showing evidence of annealing to single loci, multiple paralogous loci and probes showing evidence of probe annealing site divergence. (b) Box and whisker plots comparing the GenTrain scores of probes associated with single loci, and with multiple loci in the ‘Golden Delicious’ genome following BLAST analysis, and probes for which divergent annealing sites contained additional segregating SNPs or created null alleles.</p

Cluster plots of IRSC probes.

<p>Mean normalised Theta and normalised R values from markers genotyped in the M432 progeny using the IRSC array plotted for (a) probes to which a single significant genome match was returned and (b) probes returning multiple hits to the ‘Golden Delicious’ genome sequence.</p