Реконструкция и реставрация. Лекционный курс

Учебно-методическое пособие для студентов спец. 270302.65 «Дизайн архитектурной среды».Доступ к полному тексту открыт из сети СФУ, вне сети доступ возможен для читателей Научной библиотеки СФУ или за плату.Лекционный курс знакомит студентов с приемами реконструкции существующих территорий, зданий и сооружений, с научными принципами реставрации объектов архитектурного и исторического наследия и служит в дальнейшем теоретической основой для их творческой проектной деятельности. Предназначено для студентов специальности 270302.65 – «Дизайн архитектурной среды»

Chemical composition of pineapple sweet orange expressed as percentage composition of the major groups (averages of the different derivatization methods): A) Percentage composition of the phloem sap prepared by centrifugation method and B) Percentage composition of the the phloem sap prepared by EDTA. C) Percentage composition of phloem sap (averages from A and B).

<p>Chemical composition of pineapple sweet orange expressed as percentage composition of the major groups (averages of the different derivatization methods): A) Percentage composition of the phloem sap prepared by centrifugation method and B) Percentage composition of the the phloem sap prepared by EDTA. C) Percentage composition of phloem sap (averages from A and B).</p

Sugars and other metabolites detected in phloem sap by GC-MS after TMS derivatization (n = 5).

<p>All compounds were identified by matching their retention times and mass spectra with authentic standards. The percentages were calculated by dividing the concentration of each metabolite by the concentration of the total detected compounds then multiplying by 100.^*Quantified relative to inositol. Values are given as mean ± SD (n = 5). Different letters indicate statistically significant differences between extraction methods (<i>P</i><0.05).</p

Amino acids and organic acids detected in pineapple sweet orange phloem sap by GC-MS after MCF derivatization (n = 5).

<p>All compounds were identified by matching their retention times and mass spectra with authentic standards. The percentages were calculated by dividing the concentration of each metabolite by the concentration of the total detected compounds then multiplying by 100. Values are given as mean ± SD (n = 5). Different letters indicate statistically significant differences between extraction methods (<i>P</i><0.05).</p

Fold changes of metabolite concentrations due to the effect of <i>C</i>Las infection on <i>D</i>. <i>citri</i> nymphs and adults.

<p>Data shown is for metabolites found to be in common (detected in both nymph and adult psyllid extracts by TMS derivatization) and significantly different between healthy and <i>C</i>Las-infected psyllid populations. Metabolomic data for adult psyllids was published previously [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0191871#pone.0191871.ref014" target="_blank">14</a>].</p

Concentration (ng∙nymph^-1) of TMS-derivatized polar metabolites of <i>D</i>. <i>citri</i> nymphs reared on healthy or <i>C</i>Las-infected Valencia sweet orange flushes.

<p>Concentration (ng∙nymph^-1) of TMS-derivatized polar metabolites of <i>D</i>. <i>citri</i> nymphs reared on healthy or <i>C</i>Las-infected Valencia sweet orange flushes.</p

Concentration (ng∙nymph^-1) of MCF-derivatized polar metabolites of <i>D</i>. <i>citri</i> nymphs reared on healthy or <i>C</i>Las-infected Valencia sweet orange flushes.

<p>Concentration (ng∙nymph^-1) of MCF-derivatized polar metabolites of <i>D</i>. <i>citri</i> nymphs reared on healthy or <i>C</i>Las-infected Valencia sweet orange flushes.</p

Integration and expression of <i>CcNHX1</i> (Na⁺/H⁺ exchanger) gene in rough lemon plants.

(A) Bright field of rough lemon shoots after regeneration targeting CcNHX1 overexpression, (B) GFP-expressed shoot, (C) Amplification products obtained from PCR of transgenic rough lemon genomic DNA with 1000 bp fragment of the CcNHX1 gene, 1 kb marker. (D) Relative expression of CcNHX1 gene in transgenic lines compared to control wild type.</p

Confocal micrographs showing transient expression of NHX1-EGFP fusion protein.

Confocal images showing micrographs of transmission white light (Bright field), EGFP, chloroplast, and the resultant overlay, respectively. The EGFP panels indicate cell membrane localization of NHX1 fused to EGFP under expression of the 35S promoter. EGFP on its own was used as the positive control.</p

Fig 2 -

Cross-section of ‘Hamlin’ (A) and rough lemon (D) tissues following Agrobacterium-mediated transformation and 45 days incubation at the regeneration media. (B and C) the same ‘Hamlin’ stem section exhibiting under an epi-fluorescence stereomicroscope. (E and F) the same rough lemon stem section exhibiting under an epi-fluorescence stereomicroscope.</p