MSI-1436 compound alleviates oxidative stress in TM-treated HepG2 cells.

(A) Representative plots of cells stained with DHE evaluated using a flow cytometer. (B) Bar charts summarizing the total intracellular ROS positive cells. (C) Representative bar charts of endogenous antioxidant enzymes-related transcripts. The results are expressed as the mean of 3 different experiments ± SD. Asterisks refer to a significant difference between two groups, where: p < 0.05 (*); p < 0.01(**); p < 0.001 (***); p < 0.0001 (****). HE: Healthy untreated HepG2 cells; ERS: Endoplasmic reticulum-stressed cells; ERS+MSI: Groups pretreated with MSI-1436 compound before inducing ERS.</p

Fig_Raw_Gels images for XBP1 splicing analysis: Original uncropped gel images for XBP1 splicing obtained from agarose gel electrophoresis analysis.

Un-spliced XBP1: 281bp, spliced: 255bp. Bands were quantified using Image Studio Lite software (LI-COR Biosciences, USA). HE: Healthy untreated HepG2 cells; ERS: Endoplasmic reticulum-stressed cells; ERS+MSI: Groups pre-treated with MSI-1436 compound before inducing ERS. (PDF)</p

Table_Raw_RT-qPCR Cq Data_ER stress markers: Table summarizing the individual Cq values obtained after RT-qPCR analysis for the key ER stress associated markers.

SYBR™ Green dye–based PCR amplification and detection. Data of six technical repetitions for each experimental group. HE: Healthy untreated HepG2 cells; ERS: Endoplasmic reticulum-stressed cells; ERS+MSI: Groups pre-treated with MSI-1436 compound before inducing ERS. (PDF)</p