1 research outputs found
Association of FokI polymorphism of vitamin D receptor gene with vitamin D and parathyroid hormone levels in stage IV-V chronic kidney disease patients
511-516Most of the chronic kidney disease (CKD) stage IV-V patients have deficiency of vitamin D. Active vitamin D binds to
vitamin D receptor (VDR) to regulate target gene transcription and mediates diverse biological functions. Various genetic
variations such as FokI polymorphism, may affect the VDR gene expression and may play an important role in pathogenesis of
CKD. In this context, here, we looked into the association of FokI polymorphism of VDR gene with serum vitamin D and PTH
levels in stage IV-V CKD patients. A total of 150 patients of CKD stage IV-V, aged 25-60 years, and 150 healthy controls
(HC), age and sex matched, were enrolled for the study. FokI polymorphism was analyzed using polymerase chain reactionrestriction
fragment length polymorphism (PCR-RFLP) in the study subjects. The prevalence of different genotypes and allelic
frequency distributions were compared in study subjects. Serum PTH and vitamin D were estimated by eCLIA method. No
significant differences in genotype and in allelic frequencies between CKD patients and HC were observed. No significant
differences in biochemical parameters based on genotypic variations were observed. The results suggest no association of VDR
FokI polymorphism with CKD. No significant association was noticed between FokI variants and PTH or VitD