6 research outputs found
DataSheet1_Cucurbituril—assisted sensitive fluorescence detection and quantitation of naproxen drug in wastewater samples: Guest-host characterization and HPLC investigation.PDF
Sensitive spectrofluorometric and liquid chromatography with fluorescence detection methods have been developed for detection and determination of naproxen drug in the presence of cucurbit7uril (CB7). Fluorescence signals have been improved with the addition of CB7 to the drug aqueous solution. Fluorescence spectroscopy, mass spectrometry, 1H-NMR, and liquid chromatography with fluorescence detection were used to investigate the guest-host interaction of naproxen drug and cucurbiturils. Naproxen was found to form a supramolecular complex with CB7 that had a high formation constant. The optimal conditions for the interaction were discovered using spectroflurometry to be 0.2 mg/ml of CB7, 2.4 μg/ml of naproxen drug, and pH10. A 1:1 complex between naproxen and CB7 is revealed by proton NMR and tandem mass spectrometry. Using the standard addition calibration method, an HPLC with a fluorescence detector was used to detect naproxen in influent and effluent wastewater samples. Finally, it was discovered that the measured concentrations of naproxen in the influent and the effluent wastewater were 1.87 × 10−4 ppb and 2.1 × 10−5 ppb, respectively. This was done by sample enrichment, which reduced the 1000 mL into 1 ml.</p
DataSheet2_Cucurbituril—assisted sensitive fluorescence detection and quantitation of naproxen drug in wastewater samples: Guest-host characterization and HPLC investigation.docx
Sensitive spectrofluorometric and liquid chromatography with fluorescence detection methods have been developed for detection and determination of naproxen drug in the presence of cucurbit7uril (CB7). Fluorescence signals have been improved with the addition of CB7 to the drug aqueous solution. Fluorescence spectroscopy, mass spectrometry, 1H-NMR, and liquid chromatography with fluorescence detection were used to investigate the guest-host interaction of naproxen drug and cucurbiturils. Naproxen was found to form a supramolecular complex with CB7 that had a high formation constant. The optimal conditions for the interaction were discovered using spectroflurometry to be 0.2 mg/ml of CB7, 2.4 μg/ml of naproxen drug, and pH10. A 1:1 complex between naproxen and CB7 is revealed by proton NMR and tandem mass spectrometry. Using the standard addition calibration method, an HPLC with a fluorescence detector was used to detect naproxen in influent and effluent wastewater samples. Finally, it was discovered that the measured concentrations of naproxen in the influent and the effluent wastewater were 1.87 × 10−4 ppb and 2.1 × 10−5 ppb, respectively. This was done by sample enrichment, which reduced the 1000 mL into 1 ml.</p
Model for Photoinduced Bending of Slender Molecular Crystals
The growing realization that photoinduced
bending of slender photoreactive
single crystals is surprisingly common has inspired researchers to
control crystal motility for actuation. However, new mechanically
responsive crystals are reported at a greater rate than their quantitative
photophysical characterization; a quantitative identification of measurable
parameters and molecular-scale factors that determine the mechanical
response has yet to be established. Herein, a simple mathematical
description of the quasi-static and time-dependent photoinduced bending
of macroscopic single crystals is provided. This kinetic model goes
beyond the approximate treatment of a bending crystal as a simple
composite bilayer. It includes alternative pathways for excited-state
decay and provides a more accurate description of the bending by accounting
for the spatial gradient in the product/reactant ratio. A new crystal
form (space group <i>P</i>2<sub>1</sub>/<i>n</i>) of the photoresponsive azo-dye Disperse Red 1 (<b>DR1</b>) is analyzed within the constraints of the aforementioned model.
The crystal bending kinetics depends on intrinsic factors (crystal
size) and external factors (excitation time, direction, and intensity)
Bioinspired Molecular Lantern: Tuning the Firefly Oxyluciferin Emission with Host–Guest Chemistry
Fireflies
generate flashes of visible light via luciferase-catalyzed
chemiexcitation of the substrate (luciferin) to the first excited
state of the emitter (oxyluciferin). Microenvironment effects are
often invoked to explain the effects of the luciferase active pocket
on the emission; however, the exceedingly complex spectrochemistry
and synthetic burdens have precluded elucidation of the nature of
these interactions. To decipher the effects of microenvironment on
the light emission, here the hydrophobic interior of cucurbit[7]Âuril
(CB7) is used to mimic the nonpolar active pocket of luciferase. The
hydrophobic interior of CB7 induces shifts of the ground-state p<i>K</i><sub>a</sub>s by 1.9–2.5 units to higher values.
Upon sequestration, the emission maxima of neutral firefly oxyluciferin
and its conjugate monodeprotonated base are blue-shifted by 40 and
39 nm, respectively, resulting in visual color changes of the emitted
light
Redox-Responsive Viologen-Mediated Self-Assembly of CB[7]-Modified Patchy Particles
Sulfonated
surface patches of polyÂ(styrene)-based
colloidal particles (CPs) were functionalized
with cucurbit[7]Âuril (CB[7]). The macrocycles served as recognition
units for diphenyl viologen (DPV<sup>2+</sup>), a rigid bridging ligand.
The addition of DPV<sup>2+</sup> to aqueous suspensions of the particles
triggered the self-assembly of short linear and branched chainlike
structures. The self-assembly mechanism is based on hydrophobic/ion-charge
interactions that are established between DPV<sup>2+</sup> and surface-adsorbed
CB[7]. DPV<sup>2+</sup> guides the self-assembly of the CPs by forming
a ternary DPV<sup>2+</sup>⊂(CBÂ[7])<sub>2</sub> complex in which
the two CB[7] macrocycles are attached to two different particles.
Viologen-driven particle assembly was found to be both directional
and reversible. Whereas sodium chloride triggers irreversible particle
disassembly, the one-electron reduction of DPV<sup>2+</sup> with sodium
dithionite causes disassembly that can be reversed via air oxidation.
Thus, this bottom-up synthetic supramolecular approach allowed for
the reversible formation and directional alignment of a 2D colloidal
material
Redox-Responsive Viologen-Mediated Self-Assembly of CB[7]-Modified Patchy Particles
Sulfonated
surface patches of polyÂ(styrene)-based
colloidal particles (CPs) were functionalized
with cucurbit[7]Âuril (CB[7]). The macrocycles served as recognition
units for diphenyl viologen (DPV<sup>2+</sup>), a rigid bridging ligand.
The addition of DPV<sup>2+</sup> to aqueous suspensions of the particles
triggered the self-assembly of short linear and branched chainlike
structures. The self-assembly mechanism is based on hydrophobic/ion-charge
interactions that are established between DPV<sup>2+</sup> and surface-adsorbed
CB[7]. DPV<sup>2+</sup> guides the self-assembly of the CPs by forming
a ternary DPV<sup>2+</sup>⊂(CBÂ[7])<sub>2</sub> complex in which
the two CB[7] macrocycles are attached to two different particles.
Viologen-driven particle assembly was found to be both directional
and reversible. Whereas sodium chloride triggers irreversible particle
disassembly, the one-electron reduction of DPV<sup>2+</sup> with sodium
dithionite causes disassembly that can be reversed via air oxidation.
Thus, this bottom-up synthetic supramolecular approach allowed for
the reversible formation and directional alignment of a 2D colloidal
material