Pulmonary function in patients with Huntington\u27s Disease

Background: Huntington\u27s disease (HD) is a neurodegenerative disorder characterized by progressive motor, cognitive and psychiatric disturbances. Chest muscle rigidity, respiratory muscle weakness, difficulty in clearing airway secretions and swallowing abnormalities have been described in patients with neurodegenerative disorders including HD. However limited information is available regarding respiratory function in HD patients. The purpose of this study was to investigate pulmonary function of patients with HD in comparison to healthy volunteers, and its association with motor severity.Methods: Pulmonary function measures were taken from 18 (11 male, 7 female) manifest HD patients (53 ± 10 years), and 18 (10 male, 8 female) healthy volunteers (52 ± 11 years) with similar anthropometric and life-style characteristics to the recruited HD patients. Motor severity was quantified by the Unified Huntington\u27s Disease Rating Scale-Total Motor Score (UHDRS-TMS). Maximum respiratory pressure was measured on 3 separate days with a week interval to assess test-retest reliability.Results: The test-retest reliability of maximum inspiratory and expiratory pressure measurements was acceptable for both HD patient and control groups (ICC ≥0.92), but the values over 3 days were more variable in the HD group (CV \u3c 11.1%) than in the control group (CV \u3c 7.6%). The HD group showed lower respiratory pressure, forced vital capacity, peak expiratory flow and maximum voluntary ventilation than the control group (p \u3c 0.05). Forced vital capacity, maximum voluntary ventilation and maximum respiratory pressures were negatively (r = -0.57; -0.71) correlated with the UHDRS-TMS (p \u3c 0.05).Conclusion: Pulmonary function is decreased in manifest HD patients, and the magnitude of the decrease is associated with motor severity

Cross-education and detraining effects of eccentric vs. concentric resistance training of the elbow flexors

Background: Unilateral resistance training increases the strength of the contralateral non-trained homologous muscles known as the cross-education effect. We tested the hypothesis that unilateral eccentric resistance training (ET) would induce greater and longer-lasting cross-education effect when compared with concentric resistance training (CT). Methods: Young (20–23 y) participants were allocated to ET (5 males, 4 females) or CT (5 males, 4 females) group that performed unilateral progressive ET or CT of the elbow flexors, twice a week for 5 weeks (10 sessions) followed by a 5-week detraining, and control group (7 males, 6 females) that did not perform any training. Maximum voluntary isometric contraction torque of the elbow flexors (MVIC), one-repetition maximum of concentric dumbbell curl (1-RM), and biceps brachii and brachialis muscle thickness (MT) were measured from the trained and non-trained arms before, several days after the last training session, and 5 weeks later. A ratio between the trained and non-trained arms for the change in MVIC or 1-RM from pre- to post-training (cross-body transfer ratio) was compared between ET and CT groups. Results: The control group did not show significant changes in any variables. Both ET and CT increased (P \u3c 0.05) MVIC (22.5 ± 12.3 % vs. 26.0 ± 11.9 %) and 1-RM (28.8 ± 6.6 % vs. 35.4 ± 12.9 %) of the trained arm without a significant difference between groups. MVIC was maintained after detraining for ET but returned to the baseline for CT, and 1-RM was maintained after detraining for both ET and CT. For the non-trained arm, MVIC (22.7 ± 17.9 % vs. 12.2 ± 10.2 %) and 1-RM (19.9 ± 14.6 % vs. 24.0 ± 10.6 %) increased similarly (P \u3e 0.05) after ET and CT, and MVIC returned to the baseline after detraining, but 1-RM was maintained for both groups. An increase (P \u3c 0.05) in MT was found only after ET for the trained arm (7.1 ± 6.1 %). The cross-body transfer ratio for MVIC was greater (P \u3c 0.05) for ET (90.9 ± 46.7 %) than CT (49.0 ± 30.0 %). Conclusions: These results did not support the hypothesis and showed similar changes in the most of the variables between ET and CT for the trained and non-trained arms, and strong cross-education effects on MVIC and 1-RM, but less detraining effect after ET than CT on MVIC of the trained arm. Trial registration: University Hospital Medical Information Network Clinical Trials Registry (UMIN000044477; Jun 09, 2021)

Ex vivo reconstitution of fetal oocyte development in humans and cynomolgus monkeys

ヒト・サルの胎児卵巣から原始卵胞を体外で作出することに成功. 京都大学プレスリリース. 2022-08-01.New egg recipe to boost fertility research. 京都大学プレスリリース. 2022-08-14.In vitro oogenesis is key to elucidating the mechanism of human female germ-cell development and its anomalies. Accordingly, pluripotent stem cells have been induced into primordial germ cell-like cells and into oogonia with epigenetic reprogramming, yet further reconstitutions remain a challenge. Here, we demonstrate ex vivo reconstitution of fetal oocyte development in both humans and cynomolgus monkeys (Macaca fascicularis). With an optimized culture of fetal ovary reaggregates over three months, human and monkey oogonia enter and complete the first meiotic prophase to differentiate into diplotene oocytes that form primordial follicles, the source for oogenesis in adults. The cytological and transcriptomic progressions of fetal oocyte development in vitro closely recapitulate those in vivo. A comparison of single-cell transcriptomes among humans, monkeys, and mice unravels primate-specific and conserved programs driving fetal oocyte development, the former including a distinct transcriptomic transformation upon oogonia-to-oocyte transition and the latter including two active X chromosomes with little X-chromosome upregulation. Our study provides a critical step forward for realizing human in vitro oogenesis and uncovers salient characteristics of fetal oocyte development in primates

Muscle damage after a tennis match in young players

The present study investigated changes in indirect markers of muscle damage following a simulated tennis match play using nationally ranked young (17.6 ± 1.4 years) male tennis players. Ten young athletes played a 3-hour simulated match play on outdoor red clay courts following the International Tennis Federation rules. Muscle soreness, plasma creatine kinase activity (CK), serum myoglobin concentration (Mb), one repetition maximum (1RM) squat strength, and squat jump (SJ) and counter movement jump (CMJ) heights were assessed before, immediately after, and 24 and 48 h after the simulated match play. All parameters were also evaluated in a non-exercised group (control group). A small increase in the indirect markers of muscle damage (muscle soreness, CK and Mb) was detected at 24-48 hours post-match (p\u3c0.05). A marked acute decrement in neuromuscular performance (1RM squat strength: -35.2 ± 10.4%, SJ: -7.0 ± 6.0%, CMJ: -10.0 ± 6.3%) was observed immediately post-match (p\u3c0.05). At 24 h post-match, the 1RM strength and jump heights were not significantly different from the baseline values. However, several players showed a decrease of these measures at 24 h after the match play. The simulated tennis match play induced mild muscle damage in young players. Coaches could monitor changes in the indirect markers of muscle damage to assess athletes\u27 recovery status during training and competition

Validity of various methods for determining velocity, force, and power in the back squat

Purpose: To examine the validity of 2 kinematic systems for assessing mean velocity (MV), peak velocity (PV), mean force (MF), peak force (PF), mean power (MP), and peak power (PP) during the full-depth free-weight back squat performed with maximal concentric effort. Methods: Ten strength-trained men (26.1 ± 3.0 y, 1.81 ± 0.07 m, 82.0 ± 10.6 kg) performed three 1-repetition-maximum (1RM) trials on 3 separate days, encompassing lifts performed at 6 relative intensities including 20%, 40%, 60%, 80%, 90%, and 100% of 1RM. Each repetition was simultaneously recorded by a PUSH band and commercial linear position transducer (LPT) (GymAware [GYM]) and compared with measurements collected by a laboratory-based testing device consisting of 4 LPTs and a force plate. Results: Trials 2 and 3 were used for validity analyses. Combining all 120 repetitions indicated that the GYM was highly valid for assessing all criterion variables while the PUSH was only highly valid for estimations of PF (r = .94, CV = 5.4%, ES = 0.28, SEE = 135.5 N). At each relative intensity, the GYM was highly valid for assessing all criterion variables except for PP at 20% (ES = 0.81) and 40% (ES = 0.67) of 1RM. Moreover, the PUSH was only able to accurately estimate PF across all relative intensities (r = .92–.98, CV = 4.0–8.3%, ES = 0.04–0.26, SEE = 79.8–213.1 N). Conclusions: PUSH accuracy for determining MV, PV, MF, MP, and PP across all 6 relative intensities was questionable for the back squat, yet the GYM was highly valid at assessing all criterion variables, with some caution given to estimations of MP and PP performed at lighter loads