64 research outputs found
DON content in oat grains in Norway related to weather conditions at different growth stages
High concentrations of the mycotoxin deoxynivalenol (DON), produced by Fusarium graminearum have occurred frequently in Norwegian oats recently. Early prediction of DON levels is important for farmers, authorities and the Cereal Industry. In this study, the main weather factors influencing myco-toxin accumulation were identified and two models to predict the risk of DON in oat grains in Norway were developed: (1) as a warning system for farmers to decide if and when to treat with fungicide, and (2) for authorities and industry to use at harvest to identify potential food safety problems. Oat grain samples from farmers’ fields were collected together with weather data (2004–2013)
A mathematical model was developed and used to esti-
mate phenology windows of growth stages in oats (til-
lering, flowering etc.). Weather summarisations were
then calculated within these windows, and the Spearman
rank correlation factor calculated between DON-
contamination in oats at harvest and the weather
summarisations for each phenological window. DON
contamination was most clearly associated with the
weather conditions around flowering and close to har-
vest. Warm, rainy and humid weather during and around
flowering increased the risk of DON accumulation in
oats, as did dry periods during germination/seedling
growth and tillering. Prior to harvest, warm and humid
weather conditions followed by cool and dry conditions
were associated with a decreased risk of DON accumu-
lation. A prediction model, including only pre-flowering
weather conditions, adequately forecasted risk of DON
contamination in oat, and can aid in decisions about
fungicide treatments
Sensing of Dietary Lipids by Enterocytes: A New Role for SR-BI/CLA-1
BACKGROUND: The intestine is responsible for absorbing dietary lipids and delivering them to the organism as triglyceride-rich lipoproteins (TRL). It is important to determine how this process is regulated in enterocytes, the absorptive cells of the intestine, as prolonged postprandial hypertriglyceridemia is a known risk factor for atherosclerosis. During the postprandial period, dietary lipids, mostly triglycerides (TG) hydrolyzed by pancreatic enzymes, are combined with bile products and reach the apical membrane of enterocytes as postprandial micelles (PPM). Our aim was to determine whether these micelles induce, in enterocytes, specific early cell signaling events that could control the processes leading to TRL secretion. METHODOLOGY/PRINCIPAL FINDINGS: The effects of supplying PPM to the apex of Caco-2/TC7 enterocytes were analyzed. Micelles devoid of TG hydrolysis products, like those present in the intestinal lumen in the interprandial period, were used as controls. The apical delivery of PPM specifically induced a number of cellular events that are not induced by interprandial micelles. These early events included the trafficking of apolipoprotein B, a structural component of TRL, from apical towards secretory domains, and the rapid, dose-dependent activation of ERK and p38MAPK. PPM supply induced the scavenger receptor SR-BI/CLA-1 to cluster at the apical brush border membrane and to move from non-raft to raft domains. Competition, inhibition or knockdown of SR-BI/CLA-1 impaired the PPM-dependent apoB trafficking and ERK activation. CONCLUSIONS/SIGNIFICANCE: These results are the first evidence that enterocytes specifically sense postprandial dietary lipid-containing micelles. SR-BI/CLA-1 is involved in this process and could be a target for further study with a view to modifying intestinal TRL secretion early in the control pathway
Pathobiology of tobacco smoking and neurovascular disorders: untied strings and alternative products
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