Expression von Prostataspezifischem Membran-Antigen (PSMA) in tumorassoziierten Gefäßen beim Synovialsarkom

Ziel: Charakterisierung tumorassoziierter PSMA-Expression in der Neovaskulatur beim Synovialsarkom (SynS). Material und Methoden: Von 37 SynS-Patienten wurden relevante klinisch-pathologische Parameter erhoben und von Tumorproben Tissue Micro Arrays erstellt. Diese wurden immunhistochemisch auf Gefäßdichte und PSMA-Expressionsmuster untersucht. Die Gefäßdichte wurde mittels Micro Vessel Density (MVD) Verfahren bestimmt. Endothelzellen wurden in vivo mittels Western Blot auf PSMA-Expression untersucht. Ergebnis: Bei 37,1% der SynS wurde neovaskuläre PSMA-Expression festgestellt. Je höher die tumorassoziierte Gefäßdichte, desto höher war auch die PSMA-Expression (MVD PSMApos = 160,7+/-14,7; MVD PSMAneg = 67,5+/-8,5; p<0,0001). PSMA-induzierender Crosstalk zwischen Endothel- und Tumorzellen ließ sich in vitro nicht nachweisen. Fazit: Die PSMA-Expression in tumorassoziierter Neovaskulatur stellt einen vielversprechenden Ansatz bezüglich Diagnostik, Therapie und Rezidiverkennung des SynS dar

Arthur Bispo do Rosário a arte bruta e a propagação na cultura pós-moderna

O biográfico como representação de alteridades será fonte de explanação neste estudo, cujo interesse advém da considerável repercussão da produção artística de Arthur Bispo do Rosário no circuito cultural contemporâneo e da peculiar execução e expressão de sua obra, produzida mediante diretrizes da atividade delirante, tão proeminentes no decurso de sua psicose

GWAS and colocalization analyses implicate carotid intima-media thickness and carotid plaque loci in cardiovascular outcomes.

Carotid artery intima media thickness (cIMT) and carotid plaque are measures of subclinical atherosclerosis associated with ischemic stroke and coronary heart disease (CHD). Here, we undertake meta-analyses of genome-wide association studies (GWAS) in 71,128 individuals for cIMT, and 48,434 individuals for carotid plaque traits. We identify eight novel susceptibility loci for cIMT, one independent association at the previously-identified PINX1 locus, and one novel locus for carotid plaque. Colocalization analysis with nearby vascular expression quantitative loci (cis-eQTLs) derived from arterial wall and metabolic tissues obtained from patients with CHD identifies candidate genes at two potentially additional loci, ADAMTS9 and LOXL4. LD score regression reveals significant genetic correlations between cIMT and plaque traits, and both cIMT and plaque with CHD, any stroke subtype and ischemic stroke. Our study provides insights into genes and tissue-specific regulatory mechanisms linking atherosclerosis both to its functional genomic origins and its clinical consequences in humans

Epidemiology of intra-abdominal infection and sepsis in critically ill patients: “AbSeS”, a multinational observational cohort study and ESICM Trials Group Project

Purpose: To describe the epidemiology of intra-abdominal infection in an international cohort of ICU patients according to a new system that classifies cases according to setting of infection acquisition (community-acquired, early onset hospital-acquired, and late-onset hospital-acquired), anatomical disruption (absent or present with localized or diffuse peritonitis), and severity of disease expression (infection, sepsis, and septic shock). Methods: We performed a multicenter (n = 309), observational, epidemiological study including adult ICU patients diagnosed with intra-abdominal infection. Risk factors for mortality were assessed by logistic regression analysis. Results: The cohort included 2621 patients. Setting of infection acquisition was community-acquired in 31.6%, early onset hospital-acquired in 25%, and late-onset hospital-acquired in 43.4% of patients. Overall prevalence of antimicrobial resistance was 26.3% and difficult-to-treat resistant Gram-negative bacteria 4.3%, with great variation according to geographic region. No difference in prevalence of antimicrobial resistance was observed according to setting of infection acquisition. Overall mortality was 29.1%. Independent risk factors for mortality included late-onset hospital-acquired infection, diffuse peritonitis, sepsis, septic shock, older age, malnutrition, liver failure, congestive heart failure, antimicrobial resistance (either methicillin-resistant Staphylococcus aureus, vancomycin-resistant enterococci, extended-spectrum beta-lactamase-producing Gram-negative bacteria, or carbapenem-resistant Gram-negative bacteria) and source control failure evidenced by either the need for surgical revision or persistent inflammation. Conclusion: This multinational, heterogeneous cohort of ICU patients with intra-abdominal infection revealed that setting of infection acquisition, anatomical disruption, and severity of disease expression are disease-specific phenotypic characteristics associated with outcome, irrespective of the type of infection. Antimicrobial resistance is equally common in community-acquired as in hospital-acquired infection. © 2019, The Author(s)