Bi-layered collagen nano-structured membrane prototype (collagen matrix 10826(\uae) ) for oral soft tissue regeneration: an "in vitro" study.

AIM: To evaluate fundamental cell functions, such as adhesion, IL-6 production and proliferation of human gingival keratinocytes cultured on a newly engineered collagen matrix (CM-10826) and to assess the degree of specific biocompatibility of this new device. MATERIALS AND METHODS: Primary cultures of human keratinocytes were derived "in vitro" from biopsies of independent donors. Their true epithelial origin was ensured by the expression of cytokeratin 14. Adhesion, proliferation and production of IL-6 cytokine was then measured in the presence or absence of CM-10826 activity or of its relevant components. RESULTS: Functional tests revealed that keratinocytes adhered to CM-10826 and up-regulated their basal IL-6 production. The type of keratinocytes used expressed cytokeratin 14. Proliferation experiments demonstrated that the best cellular response was observed in the presence of Collagen I, the main component of CM-10826. No undesired effects were observed as for keratinocyte viability, morphology or differentiation. CONCLUSIONS: Our results demonstrate that CM-10826 has a favourable biological effect on the "in vitro" response of gingival keratinocytes in terms of IL-6 production, cell growth and adhesion. These findings may encourage a possible use of this collagen membrane as a tissue which, alone, may substitute for autologous gingival grafts thereby overcoming the limitations of autologous tissue

nuove prospettive in implanto-protesi e in chirurgia rigenerativa

Trattazione sulle nuove tecniche applicabili all'implantologia osteointegrata noch\ue8 sui nuovi strumenti che la tecnologia computer-assistita e la scienza dei bio-materiali offrono al clinico

Le lesioni mucose del cavo orale: elementi di diagnosi differenziale

Il testo propone un approccio clinico all'inquadramento ed alla diagnosi delle lesioni mucose del cavo orale attraverso numerosi esempi iconografici e suddividendo le lesioni per colore (bianche, rosse, nere) e per sede (guancia, palato, labbra, lingua, pavimento orale, gengiva