Optimisation of enzymatic esterification of soybean oil deodoriser distillate

The enzymatic esterification of free fatty acids from soybean oil deodoriser distillate with ethanol, catalysed by immobilised fungal lipase, was studied. The extent of conversion of free fatty acids to ethyl esters was optimised using a response surface methodology obtained through a second-order factorial experimental design. The variables studied were reaction temperature (30-70 degreesC), enzyme concentration (7-23%) and ethanol/free fatty acid molar ratio (0.3-3.7:1). The optimal reaction conditions achieved were temperature from 46.4 to 53.6 degreesC, enzyme concentration from 13.6 to 16.5% and ethanol/free fatty acid molar ratio from 1.7 to 2.3:1, with conversions above 88%. No significant tocopherol losses were observed during the process. In conclusion, enzymatic fatty acid esterification was shown to be a technically viable process. (C) 2001 Society of Chemical Industry.81121193119

Optimization of direct acid esterification process of soybean oil deodorizer distillate

In this research work, the reaction conditions for the direct acid esterification of free fatty acid from soybean oil deodorizer distillate was optimized, using anhydrous ethyl alcohol and concentrated sulfuric acid as catalyst to determine the best process conditions to get the best conversion rates of free fatty acids to ethylic esters, preserving the tocopherols. The conversion extent of free fatty acids to ethyl esters was optimized using a Response Surface Methodology obtained through a second order factorial experimental design. The optima conditions for the direct acid esterification of soybean oil deodorizer distillate were: ethanol:free fatty acids from 6.4 to 11.21, H2SO4 concentration from 0.9 to 1.5% and reaction time from 1.3 to 2.6 h, with conversions extent above 94%. No significant tocopherol losses were observed during the process. Results showed a good fit between mathematical model and data obtained at different processing conditions, making this model predictive and statistically significant (p<0.05).53220621

Optimization of chemical esterification process of saponificated and acidulated soybean oil deodorizer distillate

In this research work, it was optimized the reaction conditions for the chemistry esterification of unsaponifiable matter in soybean oil deodorizer distillate. The process involves (i) alkaline hydrolysis of saponifiable matter; (ii) acidulation of soaps and (iii) acid catalyzed esterification of resulting total fatty acid. To determine the best process conditions to get the higher conversion rates of total fatty acids to ethylic esters, preserving the tocopherols, it was used, to analyze the results, the response surface methodology in a 2nd order factorial planning. In the step (i) saponification of soybean oil deodorizer distillate saponifiable matter using NaOH:total fatty acids molar ratio = 2:1, temperature = 80degreesC and reaction time of 40 min. In the step (ii) the resulting soaps were rapidly and stoichiometrically converted in free fatty acids with sulfuric acid (23%), using an acid molar excess of 50%, at 80degreesC, for 40 min and under atmospheric pressure. In the step (iii) the optima conditions for the esterification reaction of soybean oil deodorizer distillate were: ethanol:total fatty acids molar ratio from 6.4 to 11.21, H2SO4 concentration from 0.9 to 1.5% and reaction time from 1.3 to 2.6 h, with ethyl esters conversion rates up to 98%. The mathematical model obtained is predictive and statistically significant (p<0.05). The total tocopherol losses during the process were less than 10.5%.53221822