Expression of H3K27me3 in Tibetan Patients with Gastric Cancer and Its Significance

Objective  To investigate the expression and its significance of H3K27me3 in Tibetan patients with gastric cancer.  Methods  Clinical and pathological data were retrospectively collected from Tibetan patients with gastric cancer in the Tibet Autonomous Region People's Hospital from August 2019 to August 2021 and Tibetan non-gastric cancer patients during the same period. The expression of H3K27me3 in gastric cancer tissues, corresponding adjacent normal gastric mucosa and normal gastric mucosa of Tibetan patients with non-gastric cancer tissues was detected by immunohistochemical method, and the differences of H3K27me3 expression between gastric cancer patients with different clinical and pathological characteristics was compared.  Results  A total of 54 Tibetan gastric cancer patients and 55 Tibetan non-gastric cancer patients who met the inclusion and exclusion criteria were enrolled. H3K27me3 was localized in the nucleus, and the nucleus showed brownish-yellow granular staining when positively expressed. The high expression rate of H3K27me3 in gastric cancer tissues was significantly higher than that in adjacent normal gastric mucosa tissue[64.8%(35/54) vs. 29.6%(16/54), P < 0.001]and normal gastric mucosal tissue of Tibetan patients with non-gastric cancer[64.8%(35/54) vs. 34.5%(19/55), P=0.002], and the high expression rate of H3K27me3 in the adjacent normal gastric mucosa tissue was not significantly different from that in normal gastric mucosal tissue of Tibetan patients with non-gastric cancer(P=0.683). H3K27me3 expression in gastric cancer tissues was not related to gender, age, degree of differentiation, depth of invasion, maximum tumor size, Lauren's classification, lymph node metastasis, vascular and nerve invasion and TNM stage.  Conclusions  H3K27me3 is highly expressed in gastric cancer tissues of Tibetan people in Tibet. There is no significant difference in H3K27me3 positive expression cells in adjacent tissues and normal gastric mucosa. The significance of H3K27me3 in Tibetan patients with gastric cancer is uncertain and requires further investigations

Associations of long-term exposure to air pollution and physical activity with the risk of systemic inflammation-induced multimorbidity in Chinese adults: results from the China multi-ethnic cohort study (CMEC)

Abstract Objective Previous studies proved the effect of long-term exposure to air pollution or physical activity (PA) on the risk of systemic inflammation-induced multimorbidity (SIIM), while the evidence regarding their joint effects was rare, especially in low- and middle-income countries. Therefore, we aimed to examine the extent of interaction or joint relations of PA and air pollution with SIIM. Methods This study included 72,172 participants from China Multi-Ethnic Cohort.The average concentrations of ambient particulate matter pollutants (PM1, PM2.5, and PM10) were estimated using satellite-based random forest models. Self-reported information on a range of physical activities related to occupation, housework, commuting, and leisure activities was collected by an interviewer-administered questionnaire. A total of 11 chronic inflammatory systemic diseases were assessed based on self-reported lifetime diagnosis or medical examinations. SIIM was defined as having ≥ 2 chronic diseases related to systemic inflammation. Logistic regression models were used to assess the complex associations of air pollution particulate matter and PA with SIIM. Results We found positive associations between long-term air pollution particulates exposure and SIIM, with odds ratios (95%CI) of 1.07 (1.03 to 1.11), 1.18 (1.13 to 1.24), and 1.08 (1.05 to 1.12) per 10 µg/m3 increase in PM1, PM2.5, and PM10. No significant multiplicative interaction was found between ambient air pollutant exposure and PA on SIIM, whereas negative additive interaction was observed between long-term exposure to PM2.5 and PA on SIIM. The positive associations between low volume PA and SIIM were stronger among those exposed to high-level air pollution particulates. Compared with individuals engaged in high volume PA and exposed to low-level ambient air pollutants, those engaged in low volume PA and exposed to high-level ambient air pollutants had a higher risk of SIIM (OR = 1.49 in PM1 exposure, OR = 1.84 in PM2.5 exposure, OR = 1.19 in PM10 exposure). Conclusions Long-term (3 years average) exposure to PM1, PM2.5, and PM10 was associated with an increased risk of SIIM. The associations were modified by PA, highlighting PA’s importance in reducing SIIM for all people, especially those living in high-level air pollution regions

Clinical characteristics of the studied groups.

<p>Abbreviations: BMI, body mass index; RBC, red blood cell count, RBC; HB, hemoglobin; HCT, hematocrit; LVEF, left ventricular ejection fraction.</p><p>^<b>a</b> Data are means ± SD.</p><p>Clinical characteristics of the studied groups.</p

Correlation of allele frequencies of <i>HIF2A</i> variants in Tibetans and level of high altitude.

<p>solid circle, rs56721780-C; solid diamond, rs6756667-A; solid square, rs7589621-G; solid triangle, rs1868092-A</p

Association analysis between <i>HIF2A</i> tSNPs and levels of high altitude among native Tibetans.

<p>Abbreviations: Additive, additive model; Dominant, dominant model.</p><p><i>P</i>-values except the noted ones are calculated from χ² test.</p><p>^<b>a</b><i>P</i>-values are calculated from Fisher exact test.</p><p>^<b>b</b> Bold type denotes <i>P</i><0.05.</p><p>Association analysis between <i>HIF2A</i> tSNPs and levels of high altitude among native Tibetans.</p

Association analysis between <i>HIF1A</i> tSNPs and levels of high altitude among native Tibetans.

<p>Abbreviations: Additive, additive model; Dominant, dominant model.</p><p><i>P</i>-values except the noted ones are calculated from χ² test.</p><p>^<b>a</b><i>P</i>-values are calculated from Fisher exact test.</p><p>^<b>b</b> Bold type denotes <i>P</i><0.05.</p><p>Association analysis between <i>HIF1A</i> tSNPs and levels of high altitude among native Tibetans.</p

Allele Frequencies of the altitude-associated <i>HIF2A</i> tSNPs between populations.

<p>Abbreviations: TBT, Tibetan in Tibet; TBQ, Tibetan in Qinghai; CHB, Chinese Han in Beijing. Japanese in Tokyo, Japan.</p><p>^<b>a</b> From 1000 GENOMES, phase 1.</p><p>^<b>b</b> From Xu <i>et al</i>.2011.</p><p>^<b>c</b> From Simonson <i>et al</i>.2010.</p><p>^<b>d</b> No data.</p><p>Allele Frequencies of the altitude-associated <i>HIF2A</i> tSNPs between populations.</p