288 research outputs found

    Bloch Oscillation under a Bichromatic Laser: Quasi-Miniband Formation, Collapse, and Dynamical Delocalization and Localization

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    A novel DC and AC driving configuration is proposed for semiconductor superlattices, in which the THz AC driving is provided by an intense bichromatic cw laser. The two components of the laser, usually in the visible light range, are near but not exactly resonant with interband Wannier-Stark transitions, and their frequency difference equals the Wannier-Stark ladder spacing. Multi-photon processes with the intermediate states in the conduction (valence) band cause dynamical delocalization and localization of valence (conduction) electrons, and the corresponding formation and collapse of the quasi-minibands.Comment: 4 pages, 3 figure

    A mini-Tn5-derived transposon with reportable and selectable markers enables rapid generation and screening of insertional mutants in Gram-negative bacteria

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    We re-engineered a classic tool for mutagenesis and gene expression studies in Gram-negative bacteria. Our modified Tn5-based transposon contains multiple features that allow rapid selection for mutants, direct quantification of gene expression and straightforward cloning of the inactivated gene. The promoter-less gfp-km cassette provides selection and reporter assay depending on the activity of the promoter upstream of the transposon insertion site. The cat gene facilitates positive antibiotic selection for mutants, while the narrow R6Kγ replication origin forces transposition in recipient strains lacking the pir gene and enables cloning of the transposon flanked with the disrupted gene from the chromosome. The suicide vector pCKD100, a plasmid that could be delivered into recipient cells through biparental mating or electroporation, harbours the modified transposon. We used the transposon to mutagenize Pectobacterium versatile KD100, Pseudumonas coronafaciens PC27R and Escherichia coli 35150N. The fluorescence intensities of mutants expressing high GFP could be quantified and detected qualitatively. Transformation efficiency from conjugation ranged from 1600 to 1900 CFU per ml. We sequenced the upstream flanking regions, identified the putative truncated genes and demonstrated the restoration of the GFP phenotype through marker exchange. The mini-Tn5 transposon was also utilized to construct mutant a library of P. versatile for forward genetic screens

    Brokers, voters and clientelism

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    The book is about distributive politics. The received theories usually predict that parties and governmentswill spend scarce resources on responsive voters. And these responsive voters will be fence-sitters, people who might otherwise not turn out or vote for the party responsible for the distribution but who could be swayed by a favor or a program. Yet over and over again, the evidence seemed to tell us that not fence-sitters but firm party loyalists were the primary beneficiaries of the distributive game. Because we believed in the received theories, we discarded them only reluctantly. Like good Kuhnians, a few anomalies did not shift our paradigm. But eventually the weight of the anomalies was too much. Constructing an alternative theory was only one of the tasks we faced. Our new theory suggested new questions and new observational implications. Many parties can be decomposed into leaders and low-level operatives or brokers. If brokers play the distributive game by different rules than do their leaders, allocations of resources should come out differently when brokers are in control and when leaders are in control. (They do.) If brokers are imperfect agents of party leaders, antimachine reform movements, when they break out, may be driven as much by party leaders as by non-partisan reformers. (In several countries, they have been.) And if brokers are imperfect agents, it should be the case that they impose agency losses on parties and parties should devise elaborate techniques to monitor the brokers and minimize these losses. (We offer evidence that both are true.)Fil: Nazareno, Marcelo. Universidad Nacional de Córdoba. Facultad de Filosofía y Humanidades. Escuela de Historia; Argentina.Fil: Nazareno, Marcelo. Universidad Nacional de Córdoba. Facultad de Ciencias Sociales; Argentina.Fil: Brusco, Valeria. Universidad Nacional de Córdoba. Facultad de Ciencias Sociales; Argentina.Ciencia Polític

    Avaliação da resistência à ferrugem em progênies de cafeeiro F4 obtidas por cruzamentos de 'icatu' com catimor.

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    Com o objetivo de selecionar progênies de cafeeiro resistentes à ferrugem foram instalados e conduzidos experimentos em Três Pontas, São Sebastião do Paraíso e Machado. Foram avaliadas 17 progênies desenvolvidas pelo programa de Melhoramento Genético do Cafeeiro em Minas Gerais, coordenado pela EPAMIG e obtidas pelo cruzamento ?Icatu? x Catimor, e a cultivar Rubi MG 1192 utilizada como testemunha. O delineamento foi o de blocos casualizados com três repetições. Foram analisadas as características incidência da ferrugem no primeiro semestre de 2006.Os resultados obtidos permitem verificar que as progênies avaliadas apresentam variabilidade para a resistência a ferrugem, isso é confirmado pelas estimativas da herdabilidade que foram de boa magnitude, chegando até o valor de 92,8%. Essa condição aliada à baixa incidência da doença apresentada por algumas progênies, demonstrou que é possível selecionar progênies superiores em relação à resistência a ferrugem na população estudada

    Effect of nonlinearity on the dynamics of a particle in dc field-induced systems

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    Dynamics of a particle in a perfect chain with one nonlinear impurity and in a perfect nonlinear chain under the action of dc field is studied numerically. The nonlinearity appears due to the coupling of the electronic motion to optical oscillators which are treated in adiabatic approximation. We study for both the low and high values of field strength. Three different range of nonlinearity is obtained where the dynamics is different. In low and intermediate range of nonlinearity, it reduces the localization. In fact in the intermediate range subdiffusive behavior in the perfect nonlinear chain is obtained for a long time. In all the cases a critical value of nonlinear strength exists where self-trapping transition takes place. This critical value depends on the system and the field strength. Beyond the self-trapping transition nonlinearity enhances the localization.Comment: 9 pages, Revtex, 6 ps figures include

    Surface albedo from Landsat 5 images in areas of sugar cane and cerrado

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    Propôs-se, neste trabalho, estimar dados de albedo à superfície terrestre usando-se o sensor Thematic Mapper (TM) do satélite LANDSAT 5 e compará-lo com dados de duas estações agrometeorológicas localizadas em região de Cerrado e a outra em cultivo da cana-de-açúcar. A região de estudo está localizada no município de Santa Rita do Passa Quatro, SP, Brasil. Para a realização do estudo obtiveram-se seis imagens orbitais do satélite Landsat 5 sensores TM, na órbita 220 e ponto 75, nas datas de 22/02, 11/04, 29/05, 01/08, 17/08 e 21/11, todas do ano de 2005, a que correspondem os dias juliano de 53, 101, 149, 213, 229 e 325, respectivamente. As correções geométricas para as imagens foram realizadas e geradas as cartas de albedo. O algoritmo SEBAL estimou satisfatoriamente os valores de albedo de superfícies sobre áreas de cerrado e de cana-de-açúcar, na região de Santa Rita do Passa Quatro, SP, consistentes com observações realizadas do albedo à superfície.This study aimed to estimate albedo data from the land surface sensor using the images of Thematic Mapper (TM) satellite LANDSAT 5 and to compare it with data from two agrometeorological stations located in the region of Cerrado, and another in sugar cane cultivation. The study area is located in the municipality of Santa Rita do Passa Quatro - SP, Brazil. To carry out the study six orbital images were obtained from the satellite Landsat 5 TM sensors in the orbit 220 and in the section 75, for the dates of 22/02, 11/04, 29/05, 01/08, 17/08 and 21/11 (all in the year of 2005) which correspond to the days 53, 101, 149, 213, 229 and 325, respectively. The geometric correction for images was performed and the letters of albedo were generated. The algorithm SEBAL estimated, satisfactorily, the values of albedo on the surface areas of Cerrado and sugar cane in the region of Santa Rita do Passa Quatro - SP, consistent with observations made of the surface albedo

    Exact Solution of an Evolutionary Model without Ageing

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    We introduce an age-structured asexual population model containing all the relevant features of evolutionary ageing theories. Beneficial as well as deleterious mutations, heredity and arbitrary fecundity are present and managed by natural selection. An exact solution without ageing is found. We show that fertility is associated with generalized forms of the Fibonacci sequence, while mutations and natural selection are merged into an integral equation which is solved by Fourier series. Average survival probabilities and Malthusian growth exponents are calculated indicating that the system may exhibit mutational meltdown. The relevance of the model in the context of fissile reproduction groups as many protozoa and coelenterates is discussed.Comment: LaTeX file, 15 pages, 2 ps figures, to appear in Phys. Rev.

    Dynamics of an electron in finite and infinite one dimensional systems in presence of electric field

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    We study,numerically, the dynamical behavior of an electron in a two site nonlinear system driven by dc and ac electric field separately. We also study, numerically, the effect of electric field on single static impurity and antidimeric dynamical impurity in an infinite 1D chain to find the strength of the impurities. Analytical arguments for this system have also been given.Comment: File Latex, 8 Figures available on reques

    Natural terpenoids from Ambrosia species are active in vitro and in vivo against human pathogenic trypanosomatids

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    Among the natural compounds, terpenoids play an important role in the drug discovery process for tropical diseases. The aim of the present work was to isolate antiprotozoal compounds from Ambrosia elatior and A. scabra. The sesquiterpene lactone (STL) cumanin was isolated from A. elatior whereas two other STLs, psilostachyin and cordilin, and one sterol glycoside, daucosterol, were isolated from A. scabra. Cumanin and cordilin were active against Trypanosoma cruzi epimastigotes showing 50% inhibition concentrations (IC50) values of 12 µM and 26 µM, respectively. Moreover, these compounds are active against bloodstrean trypomastigotes, regardless of the T. cruzi strain tested. Psilostachyin and cumanin were also active against amastigote forms with IC50 values of 21 µM and 8 µM, respectively. By contrast, daucosterol showed moderate activity on epimastigotes and trypomastigotes and was inactive against amastigote forms. We also found that cumanin and psilostachyin exhibited an additive effect in their trypanocidal activity when these two drugs were tested together. Cumanin has leishmanicidal activity with growth inhibition values greater than 80% at a concentration of 5 µg/ml (19 µM), against both L. braziliensis and L. amazonensis promastigotes. In an in vivo model of T. cruzi infection, cumanin was more active than benznidazole, producing an 8-fold reduction in parasitemia levels during the acute phase of the infection compared with the control group, and more importantly, a reduction in mortality with 66% of the animals surviving, in comparison with 100% mortality in the control group. Cumanin also showed nontoxic effects at the doses assayed in vivo, as determined using markers of hepatic damage.Fil: Sülsen, Valeria P.. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco (i); ArgentinaFil: Cazorla, Silvia Ines. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral "Profesor R. A. Margni"; ArgentinaFil: Frank, Fernanda Maria. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral "Profesor R. A. Margni"; ArgentinaFil: Laurella, Laura C.. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco (i); ArgentinaFil: Muschietti, Liliana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco (i); ArgentinaFil: Catalan, Cesar Atilio Nazareno. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Instituto de Quimica del Noroeste; ArgentinaFil: Martino, Virginia Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco (i); ArgentinaFil: Malchiodi, Emilio Luis. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral "Profesor R. A. Margni"; Argentin
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