EFFECT OF LATEX AND EXTRACT OF JATROPHA CURCA LINN ON EXPRESSION OF SUBSTANCE P (SP) AND CYCLOOXYGENASE-2 OF DENTAL PULP: EFEK LATEKS DAN EKSTRAK JATROPHA CURCA LINN TERHADAP EKSPRESI SUBSTANSI P DAN COX2 PULPA GIGI

Inflammatory pulpal pain may arise due to the increased pressure inside the pulp or the release of prostaglandin E2(PGE2). In the inflamed pulp, levels of PGE2 and substance P (SP) is higher than those of normal pulp. PGE2 sensitizes all nociceptor while SP can induce the activity of cyclooxygenase-2 (COX- 2) and is an exitatory neurotransmitter. Jatropha curcas Linn latex is widely used for pulpal pain relief. The aim of this study was to evaluate the effect of the latex and extract of J. curcas on the dental pulp expression of COX-2 and SP. Thirty-six pulpitis-induced dental pulp of Macaca fascicularis , were divided into 3 groups: Group 1 served as controls, Group 2 was given latex, and Group 3 was given extracts. The ELISA assay was used to determine the levels of SP and COX-2. SP data was analyzed with ANOVA (p <0.05) while the COX-2 data was analyzed with Mann Whitney. The results showed that the levels of SP (pg/mL) of the control, latex, and extract group were 28.94; 26.22; 28.89 respectively, while levels of COX-2 (ng/ml) of control, latex, and extract group were 0.04; 0.08; 0.10 respectively. In conclusion , J. curcas can reduce the levels of SP, J. curcas latex has lower levels of SP than extract, but does not provide clear results in decreased levels of COX- 2. Further study requires the mechanism of SP, and the concentration of COX- 2 needs to be further investigated using different methods

APPLICATION OF GLASS IONOMER CEMENT (GIC) FOR REPAIRING DENTAL PULP BY MEASURING EXPRESSION OF DENTIN MATRIX PROTEIN-1 : APLIKASI SEMEN IONOMER KACA UNTUK PERBAIKAN PULPA GIGI DENGAN MENGUKUR EKSPRESI DENTIN MATRIKS PROTEIN-1

Glass ionomer cement (GIC) has a potential to improve the pulp by inducing Dentin Matrix Protein-1 (DMP-1) release that can mineralize dentin. This study used three types of glass ionomer cements; Conventional GIC i.e. GC Fuji IX; resin-modified glass ionomer cement (RMGIC) i.e. GC Fuji II LC and Nano particle of RMGIC i.e. Ketac™ N100 3MEspe. The three materials were applied to the tooth pulp of Macaca nemestrina. Expression of DMP-1 extract-dental pulp of the subjects was investigated by using ELISA. This study was statistically analyzed by using Mann-Whitney test. The result showed that GICs has a potential to induce the expression of DMP-1 and there was no significant differences among the three groups of GIC to induce DMP-1(p≥ 0,05). In conclucion, all tested materials have a potential in dental pulp repair by expressing DMP-1

EVALUASI DAN PENGENDALIAN FAKTOR RISIKO KARIES

Dental caries is a multifactorial disease that it is a preventable one. The assessment and control of the risk factors, therefore, plays a significant role in evading the development of caries. Risk factors include medical and dental history, clinical, radiographic, and laboratory factors. Medical and dental history factors consist of social-economic status of the patients, age, gender, oral hygiene, fluoride exposure, the dietary carbohydrate, civilizaiton, general health, and medicaiton. Clinical factors to be assessed are dental plaque, the susceptible site of the tooth, the existing restoration, and orthodontic appliances. The laboratory factors evaluate the conditions of the saliva i.e. the buffer capacity, flow rate, and the presence of caries-related microorganisms such as mutans streptococci, lactobacilli, or yeast. As caries is caused by three main factors (host, cariogenic substrate, and microflora) the control of the above mentioned factors consist of increasing the resistance of the host, evading the cariogenic substrate and microflora, and suppressing the modulator that increasing caries activity

Evaluation of Mutagenicity of Three Eugenol-Containing Materials

Eugenol containing materials are still widely used, both by the lay people or by the dentist. Professionally it is used to relieve dental pain by placing it in the cavity, or as a mixture for temporary filling, temporary cementation, and root canal sealer. Eugenol, however, is also known to be toxic to the tissue, and aflatoxin, a substance known to be mutagenic, has been found in material labeled as clove oil. The purpose of this study was to investigate the safety of these materials, in particular the mutagenicity. The materials tested were imported eugenol material (95.8%) eugenol and local eugenol material (99.5%), both used by dentists and clove-oil material (6.8%) eugenol used by the lay people. These materials were assayed in Ames test using Salmonella typhimarium TA 1535 and TA 1537 strain without S-9 mixture. It was shown that all revertants were below the negative control of the test. It was concluded that neither the eugenol used by the dentist nor the clove oil used for suppressing the dental pain by the lay people showed mutagenicity to Salmonella typhimurium TA 1535 and TA 1537 strain

Anestesia Lokal: dalam Perawatan Konservasi Gigi

viii, 124 Halaman.; 21 c

EVALUASI DAN PENGENDALIAN FAKTOR RISIKO KARIES

Dental caries is a multifactorial disease that it is a preventable one. The assessment and control of the risk factors, therefore, plays a significant role in evading the development of caries. Risk factors include medical and dental history, clinical, radiographic, and laboratory factors. Medical and dental history factors consist of social-economic status of the patients, age, gender, oral hygiene, fluoride exposure, the dietary carbohydrate, civilizaiton, general health, and medicaiton. Clinical factors to be assessed are dental plaque, the susceptible site of the tooth, the existing restoration, and orthodontic appliances. The laboratory factors evaluate the conditions of the saliva i.e. the buffer capacity, flow rate, and the presence of caries-related microorganisms such as mutans streptococci, lactobacilli, or yeast. As caries is caused by three main factors (host, cariogenic substrate, and microflora) the control of the above mentioned factors consist of increasing the resistance of the host, evading the cariogenic substrate and microflora, and suppressing the modulator that increasing caries activity

Evaluation of Mutagenicity of Three Eugenol-Containing Materials

Eugenol containing materials are still widely used, both by the lay people or by the dentist. Professionally it is used to relieve dental pain by placing it in the cavity, or as a mixture for temporary filling, temporary cementation, and root canal sealer. Eugenol, however, is also known to be toxic to the tissue, and aflatoxin, a substance known to be mutagenic, has been found in material labeled as clove oil. The purpose of this study was to investigate the safety of these materials, in particular the mutagenicity. The materials tested were imported eugenol material (95.8%) eugenol and local eugenol material (99.5%), both used by dentists and clove-oil material (6.8%) eugenol used by the lay people. These materials were assayed in Ames test using Salmonella typhimarium TA 1535 and TA 1537 strain without S-9 mixture. It was shown that all revertants were below the negative control of the test. It was concluded that neither the eugenol used by the dentist nor the clove oil used for suppressing the dental pain by the lay people showed mutagenicity to Salmonella typhimurium TA 1535 and TA 1537 strain

ENAMEL MICROHARDNESS AFTER APPLICATION OF 30% CARBAMIDE PEROXIDE AND SUBSEQUENTLY AFTER APPLICATION OF FLUORIDE

Thirty percent carbamide peroxide is widely used as in extra-coronal bleaching method. Its current effect on enamel (e.g. enamel microhardness) is still uncertain. In dentistry, fluoride is recommended to be applied in 60 seconds. The aim of this study is to observe the effect of 30% carbamide peroxide on enamel microhardness and also the effect of fluoride application on enamel microhardness that changed upon bleaching activity within shorter period than time of application recommended (30 seconds), in recommended period of application (60 seconds), and in a longer period of 90 seconds. Thirty extracted teeth were bleached with 30% carbamide peroxide for 1 hour, and the enamel microhardness was measured by Knoop method. The microhardness of enamel was also evaluated soon after application of fluoride to the bleached tooth and after fluoride application and immersion in artificial saliva for one hour. It was revealed that the microhardness of normal enamel was 360,46 KHN and decreased significantly to 170,33 KHN (57%) after application of 30% carbamide peroxide. Soon after application of fluoride there was an increase in enamel microhardness although not significant on all duration of application. After application of fluoride and one hour immersion in saliva, the enamel microhardness increases significantly, especially in duration of fluoride and one hour immersion in saliva, the enamel microhardness increased significantly, especially in duration of fluoride application longer than recommended (90 seconds). Based on data above, it can be concluded that there was a 57% decrease in enamel hardness after application of 30% carbamide peroxide and this enamel microhardness increased after application of fluoride and after immersion in artificial saliva

Kamus kedokteran gigi = (concise illustrated dental dictionary)

384 hlm. : ilus. ; tab. ; 21 cm

Mukositis Akibat Radiasi: Pencegahan dan Pengobatan

xi, 147 Halaman.; 19 c