Analytical Electron Microscopic Study of Mineral Deposits in a Case of Calcionsis Universalis

Calcinosis universalis with dermatomyositis occurred in a 58-year-old woman. Tissues removed from the patient\u27s sublingual region were studied mainly by analytical electron microscopic methods. According to an elemental analysis using a wavelength dispersive X-ray spectroscope, most of the mineral deposits contained the elements sodium, phosphorus, calcium, rhenium, and some deposits also contained sulphur and magnesium. The elements sodium, sulphur, chlorine, and calcium were found in the stromal tissues of the material. Energy dispersive X-ray spectroscopy demonstrated that the analysis-peaks of phosphorus and calcium were higher in the mineral deposits than in the stromal tissues. The mineral deposits throughout the specimen were mainly composed of hydroxyapatite, judging from field-limited area electron diffraction and X-ray diffraction examinations

Chapter 4 : Injury and the Recovery Reaction due to the Penetration of Material into the Mandibular Canal

A root canal filling material paste, mainly made of calcium hydroxide and iodoform with the addition of silicone oil, was experimentally introduced into the mandibular canals of dogs. The tissue reactions to the paste were examined by radiography, histopathology, and electron microscopy. The examination results showed that the paste was phagocytosed by macrophages and, in general, was gradually resorbed by the passage of time. The paste was also found to cause heterotopic calcification and/or bone formation within the limited area of original penetration. The histopathological tissue changes of the alveolar nerve tissue were also examined. No injury-related histopathological change was observed as long as the injected paste did not contact alveolar nerve tissue, but tissue damage was observed at sites of direct contact. After removal of degenerated nerved debris by macrophages and Schwann cells, the persisting external membrane or “Schwann tubes” appeared to provide a scaffold for axonal regeneration and Schwann cell proliferation.Editor, Toshiyuki KawakamiLanguage Editor, David M Carlsonviii, 232 p. ill. (some col.

Light and Electron Microscopic Studies of Microcalcifications Appearing in Monomorphic Adenomas

Microcalcifications appearing in two cases of monomorphic adenomas were studied histopathologically, electron microscopically, and electron-microanalytically. One case was basal cell adenoma that occurred in a 56-year-old man and the other was canalicular adenoma in a 71-year-old woman. The calcified granules were observed both in the lumina formed by the tumor cells and in the stromal tissues. The surroundings of the granules were stained by alcian blue and showed a sulfur peak by EPMA. These facts suggest that the surroundings contain sulfated glycosaminoglycans and that sulfur has a significant role in the mechanisms of pathological calcification as well as physiological calcification

Amyloid Deposition in Human Pulp

A tooth with a pulp having a rare degenerative change was found among numerous extracted tooth specimens. Microscopically eosinophilic dimunutive granules existed around vesseles in the coronal pulp. These were stained positively with Congo red and periodic acid-Schiff. Electron microscopic observation revealed that granules were composed of bundles showing one of the characteristic appearances of amyloid deposition

A Comparative Study on Histopathological Aspects of Benign and Malignant Smooth Muscle Tumors appeared in Oral Regions

Histopathological aspects of two cases of smooth muscle tumors appeared in oral regions (case 1: angiomyoma; case 2: leiomyosarcoma) have been described. Histopathological features of both cases showed a similarity in terms of relationship with blood vessels. In spite of the changes of cell structure and the destructive pattern of cell proliferation, the results as determined by special staining techniques were the same in the benign and malignant tumors. The histopathological features, origin, and differential diagnosis of the two types of tumors were also discussed

Ultrastructural Study of Varied Calcified Materials in the Pleomorphic Adenoma Occurring in the Soft Palate

A case of pleomorphic adenoma derived from the palatine gland was studied with light microscope, transmission electron microscope and X-ray microprobe analysor. Some irregular basophlic masses were found under the light microscope in the stromal tissue close to tumor cells, which were ultrastructurally composed of needle-shaped crystals and bordered by a lamina limitans-like structure. Furthermore, transmission electron microscopic findings showed that numerous membranous vesicles with appatite deposited in various degrees were scattered between collagen fibers. These vesicles seemed to be matrix vesicles which may be related to a stromal change of pleomorphic adenoma