ヒト胃癌患者におけるtripartite motif 32の過剰発現は予後不良に関連する

Tripartite motif protein (TRIM) 32 belongs to the TRIM family, which is composed of RING finger, B-box and coiled-coil domains. TRIM32 has been reported to function as an enzyme 3 ubiquitin ligase and is overexpressed in numerous types of cancer. The present study evaluated the clinical significance of TRIM32 expression levels in gastric cancer. The current study also investigated the TRIM32 expression levels in 142 patients with gastric cancer using immunohistochemistry and examined its clinical importance and potential as a prognostic marker. Furthermore, the function of TRIM32 was examined in vitro. High TRIM32 expression levels were detected in gastric cancer tissues. The postoperative overall and relapse-free survival rates were significantly reduced in patients with tumors with high levels of TRIM32 expression compared with those with tumors expressing low levels of TRIM32. Tumors expressing high levels of TRIM32 were associated with an increased risk of postoperative recurrence, particularly hematogenous recurrence. Multivariate analysis identified TRIM32 status as an independent prognostic factor. Furthermore, TRIM32 gene silencing induced apoptosis and inhibited the proliferation of gastric cancer cells in vitro. Therefore, TRIM32 expression levels may be of potential prognostic value in gastric cancer.博士（医学）・乙第1402号・平成29年6月28日Copyright © Spandidos Publications 2017. All rights reserved.The Spandidos Publications link is available at " http://dx.doi.org/10.3892/ol.2017.5806

Vision outcome with antiglaucoma therapy and prognostic factors in canine glaucoma: A 6-years retrospective study in Japan

Vision outcome provides invaluable information in clinical decision making in the management of canine glaucoma. In the present study, data of glaucoma dogs were retrospectively evaluated for vision outcome by treatment modality (with or without surgical implantation of the Ahmed glaucoma valve, AGV) and by type of glaucoma, sex and breed in cases of medically treated glaucoma. Among 1990 dogs presented with eye diseases between 2011 and 2017, 224 dogs (11.3%) were diagnosed with glaucoma at initial presentation and 228 eyes of 207 dogs have follow-up records of at least 30 days were included in the analysis. At the time of first presentation, 62/228 eyes (27.2%) were visual. Visual preservation rates were constantly significantly higher in dogs that received AGV placement with a median time to vision loss of 76.4 weeks vs. 9.6 weeks in dogs that received medical treatment alone. Among dogs treated medically, vision outcome was comparable between two types of glaucoma (i.e., primary and secondary) and between sexes. Medically treated Shiba dogs showed significantly lower vision preservation rates and a shorter median time to vision loss compared to other breeds. These results suggest that AGV implants result in better vision outcome compared to medical therapy alone and should be considered in dogs that are visual at the time of presentation and suitable for surgery. And Shiba dogs are considered as the factor that indicate poor vision outcome of medical treatment alone in the present study

Porencephaly in dogs and cats: relationships between magnetic resonance imaging (MRI) features and hippocampal atrophy

Porencephaly is the congenital cerebral defect and a rare malformation and described few MRI reports in veterinary medicine. MRI features of porencephaly are recognized the coexistence with the unilateral/bilateral hippocampal atrophy, caused by the seizure symptoms in human medicine. We studied 2 dogs and 1 cat with congenital porencephaly to characterize the clinical signs and MRI, and to discuss the associated MRI with hippocampal atrophy. The main clinical sign was the seizure symptoms, and all had hippocampal atrophy at the lesion side or the larger defect side. There is association between hippocampal atrophy or the cyst volume and the severe of clinical signs, and it is suggested that porencephaly coexists with hippocampal atrophy as well as humans in this study

Indocyanine green angiography findings with Collie eye anomaly in Hokkaido dogs

Collie eye anomaly (CEA) is an inherited, congenital ocular disorder caused by a defective mesodermal differentiation in the posterior segment of the eye. Major ocular finding of CEA is abnormalities of choroidal vessels, that is choroidal hypoplasia. Indocyanine green angiography (IA) is one of the useful ocular examination to observe choroidal vessels in both human and dogs. The purpose of this study was to evaluate IA with CEA in Hokkaido dogs, which is one of the traditional Japanese breed and natural monument in Japan. Ten Hokkaido dogs that had been carried out genetic tests in advance were included in this study. Dogs included in this study had ophthalmic examination, such as menace response, dazzle reflex, direct and indirect pupillary light reflex, slit-lamp biomicroscopy, simple funduscopy, and IA. According to the result of genetic tests, they were classified as 8 affected and 2 carrier dogs. Simple funduscopy revealed choroidal hypoplasia bilaterally and temporal or dorsotemporal area to the optic disc in all affected dogs. With IA, we could observe the abnormalities of choroidal vessels not only at the area coincided with choroidal hypoplasia with simple funduscopy but also at the area detected normal with simple funduscopy in affected dogs. No abnormalities on fundus were observed with both simple funduscopy and IA in all carrier dogs. In conclusion, it was revealed that choroidal hypoplasia in CEA Hokkaido dogs was existed also in the area that could not be observed with simple funduscopy

Effects of gel pad thickness on skin stiffness evaluation by ultrasonographic strain elastography in normal dogs

Gel pads are commonly used for skin ultrasound examination; however, the effect of their thickness on the measured values is unknown. We investigated the effect of pad thickness on skin stiffness measurement in healthy dogs. The skin stiffness of the dorsal neck was measured using a durometer. Ultrasonographic strain elastography was performed with 5-, 10-, and 20-mm-thick pads. Among the strain ratios, muscle/skin (5 mm) showed a significantly positive correlation with skin stiffness. In the strain histogram, AREA (20 mm), CONTRAST (20 mm), MEAN (5, 20 mm), and STANDARD DEVIATION (20 mm) were significantly correlated with skin stiffness. In conclusion, the thickness of the gel pad affects the measurements during skin ultrasonographic strain elastography

Low-field magnetic resonance imaging and computed tomography of a calf with aqueductal stenosis caused by web: comparison with normal calves

A 6-day-old female Holstein displayed a dome-shaped skull and cardiac murmur on physical examination. Neurological abnormalities included progressive ataxia, decreased pupillary light reflex, and blindness soon after birth. On diagnostic imaging, CT identified expanded ventricles and thyroid hypoplasia on the left side. MRI detected expanded ventricles, especially in the rostral cerebrum at the mesencephalic aqueduct, compared with normal calves, so we suspected hydrocephalus causing stenosis of the mesencephalic aqueduct. Postmortem examination revealed a structure in the mesencephalic aqueduct resembling the “web” type of aqueductal stenosis described in humans. This case report indicates the utility of describing mesencephalic aqueductal stenosis by web and detection of other malformations on CT and MRI for antemortem diagnosis in calves