HPLC DETERMINATION OF PARTHENOLIDE AND APIGENIN CONTENTS VERSUS LABEL CLAIMS IN IRANIAN PRODUCTS

In this study parthenolide content of feverfew product (Tanamigraine capsule) and apigenin content of chamomile products (Chamomill drop, Kamisol solution, Prostatan drop, Prostatan tablet) were determined as effective compound by HPLC. Feverfew product was extracted by methanol/distilled de-ionized water (50: 50) and chamomile products by methanol. The HPLC operating conditions were C-18 reversed phase column (VP-ODS 250×4.6 mm, 5 mm), methanol/ distilled de-ionized water (60: 40) as the mobile phase at a flow rate of 1 ml/min and UV detection at 214 nm for parthenolide and 2% acetic acid: acetonitril with linear program 80 to 40 % of acetic acid 2% with a flow rate of 1 ml/min and UV detection at 275nm for apigenin. The amount of parthenolide and apigenin obtained in Tanamigraine capsules and chamomile products is 0.13- 016% and 0.04- 35% respectivel

Investigating the cytotoxic effect of some medicinal plants from northern parts of Iran

Abstract Background and objectives: Iran owns a rich and prestigious heritage of medicinal herbs but the majority of these plants have not yet undergone chemical, pharmacological and toxicological studies. In the present study some species form northern parts of Iran were evaluated for cytotoxicity. Methods: Sixteen medicinal plants were extracted with methanol and screened for their cytotoxic activities. The inhibition of cell growth for these extracts was evaluated against MCF-7, WEHI-164, HepG-2 and MDBK cell lines. Their 50% inhibitions of growth (IC 50 ) were determined by MTT assay. Moreover, cytotoxic evaluation of different fractions of the most potent species was performed. Results: Among examined samples, the IC 50 values of methanol extract of Centaurea bruguierana (DC.) Hand.-Mzt. on mentioned cell lines were found 47.30-87.40 µg/mL. In addition, the chloroform fraction of the species was cytotoxic with IC 50 values 17.00-23.03 µg/mL. Conclusion: It was concluded that the chloroform fraction of C. bruguierana was the best candidate for identification and isolation of active principles with cytotoxic effects. These results recommend further studies about this species

Investigating the cytotoxic effect of some medicinal plants from northern parts of Iran

Background and objectives: Iran owns a rich and prestigious heritage of medicinal herbs but the majority of these plants have not yet undergone chemical, pharmacological and toxicological studies. In the present study some species form northern parts of Iran were evaluated for cytotoxicity. Methods: Sixteen medicinal plants were extracted with methanol and screened for their cytotoxic activities. The inhibition of cell growth for these extracts was evaluated against MCF-7, WEHI-164, HepG-2 and MDBK cell lines. Their 50% inhibitions of growth (IC50) were determined by MTT assay. Moreover, cytotoxic evaluation of different fractions of the most potent species was performed. Results:Among examined samples, the IC50 values of methanol extract of Centaurea bruguierana (DC.) Hand.-Mzt. on mentioned cell lines were found 47.30-87.40 µg/mL. In addition, the chloroform fraction of the species was cytotoxic with IC50 values 17.00-23.03 µg/mL. Conclusion: It was concluded that the chloroform fraction of C. bruguierana was the best candidate for identification and isolation of active principles with cytotoxic effects. These results recommend further studies about this species