МОРФОЛОГИЧЕСКИЕ И ИММУНОГИСТОХИМИЧЕСКИЕ ПРОЯВЛЕНИЯ РАЗЛИЧНЫХ ФОРМ ОСТРОГО ОТТОРЖЕНИЯ ПОЧЕЧНОГО АЛЛОТРАНСПЛАНТАТА

The aim of this study was morphological characteristics of peculiar properties of acute T-cells- (ATMR) and antibody-mediated kidney allograft rejection (AAMR). Histological slices of kidney allograft biopsies were examined after coloration by routine methods and immunohistochemical proceeding for C4d, CD45R0 T-lymphocytes antigen and CD68 macrophages antigen. We’ve determined that the key features of ATMR are: increasing of T-lymphocytes interstitial infi ltration with the same level of macrophages infi ltration comparing with implantation biopsies, mild mononuclear glomerulitis and capillaritis; tubulitis. Morphological traits of AAMR are neutrophilic and T-lymphocytes glomerulitis, diffuse capillaritis, capillaries dilatation and margination of mononuclear cells with admixture of neutrophils, macrophagal interstitial infi ltration under semiquantitative evaluation, as well as, more pronounced, than in ATMR, T-lymphoctes and macrophages interstitial infi ltration under quantitative evaluation.Целью исследования была морфологическая характеристика острого отторжения (ОРО) почечных аллотрансплантатов: Т-клеточно- (ОТОО) и антитело-опосредованного (ОАОО). Гистологические срезы биоптатов исследовались после их окрашивания рутинными методами, а также антителами к C4d-компоненту комплемента, CD45R0-антигену Т-лимфоцитов и CD68-антигену макрофагов. Показано, что для ОТОО характерно усиление Т-лимфоцитарной инфильтрации интерстиция по сравнению с дотрансплантационным этапом при сохранении степени макрофагальной инфильтрации, минимальный мононуклеарный гломерулит и капиллярит; тубулит. Морфологическими признаками ОАОО являются: нейтрофильный и Т-лимфоцитарный гломерулит, диффузный капиллярит с дилатацией ПТК, маргинацией моноцитов с примесью нейтрофилов, усиление по сравнению с дотрансплантационным этапом макрофагальной инфильтрации интерстиция при полуколичественной оценке; более высокая по сравнению с ОТОО степень Т-лимфоцитарной и макрофагальной инфильтрации интерстиция с преобладанием лимфоцитов при количественной оценке

MORPHOLOGICAL AND IMMUNOHISTOCHEMICAL MANIFESTATIONS OF DIFFERENT VARIANTS OF ACUTE KIDNEY ALLOGRAFT REJECTION

The aim of this study was morphological characteristics of peculiar properties of acute T-cells- (ATMR) and antibody-mediated kidney allograft rejection (AAMR). Histological slices of kidney allograft biopsies were examined after coloration by routine methods and immunohistochemical proceeding for C4d, CD45R0 T-lymphocytes antigen and CD68 macrophages antigen. We’ve determined that the key features of ATMR are: increasing of T-lymphocytes interstitial infi ltration with the same level of macrophages infi ltration comparing with implantation biopsies, mild mononuclear glomerulitis and capillaritis; tubulitis. Morphological traits of AAMR are neutrophilic and T-lymphocytes glomerulitis, diffuse capillaritis, capillaries dilatation and margination of mononuclear cells with admixture of neutrophils, macrophagal interstitial infi ltration under semiquantitative evaluation, as well as, more pronounced, than in ATMR, T-lymphoctes and macrophages interstitial infi ltration under quantitative evaluation

Crystal structures of N-[(4-phenylthiazol-2-yl)carbamothioyl]benzamide and N-{[4-(4-bromophenyl)thiazol-2-yl]carbamothioyl}benzamide from synchrotron X-ray diffraction

The title compounds, C17H13N3OS2, (I), and C17H12BrN3OS2, (II), are potential active pharmaceutical ingredients. Compound (I) comprises two almost planar fragments. The first is the central (carbamothioyl)amide (r.m.s. deviation = 0.038-Å), and the second consists of the thiazole and two phenyl rings (r.m.s. deviation = 0.053-Å). The dihedral angle between these planes is 15.17-(5)°. Unlike (I), compound (II) comprises three almost planar fragments. The first is the central N-(thiazol-2-ylcarbamothioyl)amide (r.m.s. deviation = 0.084-Å), and the two others comprise the bromophenyl and phenyl substituents, respectively. The dihedral angles between the central and two terminal planar fragments are 21.58-(7) and 17.90-(9)°, respectively. Both (I) and (II) feature an intramolecular N - H⋯O hydrogen bond, which closes an S(6) ring. In the crystal of (I), molecules form hydrogen-bonded layers parallel to (100) mediated by N - H⋯S and C - H⋯O hydrogen bonds. In the crystal of (II), molecules form a three-dimensional framework mediated by N - H⋯Br and C - H⋯O hydrogen bonds, as well as secondary S⋯Br [3.3507-(11)-Å] and S⋯S [3.4343-(14)-Å] interactions. © Wang et al. 2016

Crystal structures of N-[(4-phenylthiazol-2-yl)carbamothioyl]benzamide and N-{[4-(4-bromophenyl)thiazol-2-yl]carbamothioyl}benzamide from synchrotron X-ray diffraction

The title compounds, C17H13N3OS2, (I), and C17H12BrN3OS2, (II), are potential active pharmaceutical ingredients. Compound (I) comprises two almost planar fragments. The first is the central (carbamothioyl)amide (r.m.s. deviation = 0.038-Å), and the second consists of the thiazole and two phenyl rings (r.m.s. deviation = 0.053-Å). The dihedral angle between these planes is 15.17-(5)°. Unlike (I), compound (II) comprises three almost planar fragments. The first is the central N-(thiazol-2-ylcarbamothioyl)amide (r.m.s. deviation = 0.084-Å), and the two others comprise the bromophenyl and phenyl substituents, respectively. The dihedral angles between the central and two terminal planar fragments are 21.58-(7) and 17.90-(9)°, respectively. Both (I) and (II) feature an intramolecular N - H⋯O hydrogen bond, which closes an S(6) ring. In the crystal of (I), molecules form hydrogen-bonded layers parallel to (100) mediated by N - H⋯S and C - H⋯O hydrogen bonds. In the crystal of (II), molecules form a three-dimensional framework mediated by N - H⋯Br and C - H⋯O hydrogen bonds, as well as secondary S⋯Br [3.3507-(11)-Å] and S⋯S [3.4343-(14)-Å] interactions. © Wang et al. 2016