FEATURES OF MONOCYTE POLARIZATION AT DIFFERENT OUTCOMES IN WOMEN WITH RECURRENT MISCARRIAGE

Currently, in the pathogenesis of recurrent miscarriage, a special role is given to immunological factors, in particular the role of innate immunity. The aim of the study was to assess the relative content of monocytes in the peripheral blood producing IL-4, IL-6, IL-10, IFNγ, as well as to identify new criteria for predicting the outcome of pregnancy in women with the threat of early termination and recurrent miscarriage. Materials and methods. 88 pregnant women at 5-12 weeks’ gestation were examined, the main group consisted of 59 women with recurrent miscarriage and threatened miscarriage at the time of the study, the control group – 29 women with uncomplicated pregnancy without recurrent miscarriage. The main group, depending on the outcomes of pregnancy, was subdivided into subgroups: subgroup I – 42 women whose pregnancy ended in timely delivery, subgroup II – 8 women with preterm labor, subgroup III – 9 women with abortion up to 22 weeks (spontaneous miscarriage and non-developing pregnancy). In the control group, all women had a timely delivery. Research material – peripheral venous blood. The relative content of IL-4+, IL-6+, IL-10+, IFNγ+ monocytes was assessed on a FACSCanto II flow cytometer using monoclonal antibodies. Statistical data processing was carried out using a package of standard applied programs. Results. In the group of women with recurrent miscarriage and threatened miscarriage, the relative content of IL-10+ and IL-4+ monocytes was reduced and the content of IL-6+ monocytes was increased compared to the control group (p = 0.0001 in all cases). There were no statistically significant differences in the content of IFNγ+ monocytes in the compared groups (p = 0.069). With a relative content of IL-4+ monocytes equal to 26.7% or less, preterm labor is predicted. With a relative content of IL-10+ monocytes equal to 27.0% or less, abortion (spontaneous miscarriage or miscarriage) is predicted in gestational age up to 22 weeks. An increase in the ratio of IFNγ+/ IL-4+, IFNγ+/IL-10+, IL-6+/IL-4+, IL-6+/IL-10+ monocytes was found in the main group (p < 0.0001 in all cases ). Conclusions. In women with recurrent miscarriage in all subgroups, the level of M1 monocytes prevailed over the level of M2 monocytes. The data obtained allowed the development of new prognostic criteria for termination of pregnancy up to 22 weeks and premature birth

CURRENT OPPORTUNITIES OF SONOGRAPHIC DIAGNOSTICS TIBIAL OSTEOCHONDROMAS

Osteochondroma is the most common tumor of the skeleton. Its frequency is more than 40% of benign and at least 20% of all human bone tumors. The objective of this study was to improve methods of diagnostics of shin bone osteochondromas with using of modern imaging techniques. There were treated 81 patients with primary tibia osteochondroma and one with a secondary chondrosarcoma during the period from 2000 to 2011 in the Clinic of Traumatology and Orthopedics of the Astrakhan Medical Academy Diagnostics was based on the data of anamnesis, physical examination, standard X-rays and computed tomography. We applied ultrasound studies of shin bones in 11 patients. The ultrasonography has allowed revealing two main forms of osteochondromas: “globular-shaped” and “hill-shaped”. The tumor goes into the third “cauliflower-shaped” form in the case of malignant transformation. The distribution of the cartilage on the osteochondroma surface is irregular and depends on the tumor shape. The indirectly malignant signs are the loss of sharpness and smoothness of the contour, hyperechoic inclusions in the cartilage cap

FEATURES OF THE CONTENT OF ENDOTHELIAL PROGENITOR CELLS IN UMBILICAL CORD BLOOD OF PRE-TERM AND FULL-TERM NEWBORNS

The purpose of the study: determination of content of circulating endothelial cellsin umbilical cord blood in orderto assessthe regenerative potential of the endothelium in newborns. 29 newborn babies were examined: 18 pre-term infants (gestational age 30–35 weeks) and 11 full-term infants (gestational age 38–41 weeks). Determination of the number of circulating endothelial cells was determined in mixed umbilical cord blood of all children their phenotyping was carried out by cytofluorometry method using fluorochromes-labeled monoclonal antibodies. Circulating endothelial cells were defined as CD45–, CD133+, VEGFR2, CD34–, endothelial progenitor cells were defined as CD45–, CD133+, VEGFR2+, CD34+. It was revealed that in umbilical cord blood of pre-term infants was decreased both the total number of circulating endothelial cells and endothelial progenitor cells compared to full-term infants. Moreover, in preterm infants was observed higher percentage of endothelial progenitor cells, rather than mature endothelial cells. In blood of full-term infants was observed higher percentage of mature endothelial cells compared to endothelial progenitor cells and their number increases with increasing birth weight of the newborn. The decrease in the number of endothelial progenitor cells in pre-term infants is associated with impaired endothelial function. CD45–CD133+VEGFR2+CD34+ cells are involved in reparation of damaged endothelium and reduction in their number in the blood reflects the reduction of its regenerative potential

Анализ эффективности антибактериальной терапии у пациентов с заболеваниями органов дыхания в амбулаторной терапевтической практике

The aim of this study was to analyze the rationality of antibiotics administration and choice in patients with different respiratory diseases referred from outpatient departments to the city diagnostic center № 1 at Saint Petersburg. Two hundred and thirty five outpatient medical histories were analyzed. We revealed cases of unreasonable (in patients with bronchial asthma and exogenous allergic alveolitis) and irrational antibiotic administration (rare administration of inhibitor protected aminopenicillins, cefuroxime axetil, respiratory quinolones; use of aminoglycozides, co trimoxazolе and tetracyclines in therapeutic patients). Elderly and senile age, smoking and post tuberculotic lung pathology provided lingering pneumonia course. Eighteen patients with uncomplicated pneumonia (including lingering variants), exacerbation of chronic bronchitis and bronchiectasis were successfully treated by sparfloxacin.Целью данного исследования явился анализ обоснованности назначения антибактериальной терапии (АБТ) и рациональности выбора препаратов у больных с различными заболеваниями органов дыхания, которые были направлены на обследование и консультацию пульмонолога городского диагностического центра № 1 Санкт-Петербурга из поликлиник города. Проанализированы амбулаторные карты 235 пациентов. Были выявлены случаи необоснованного (у больных бронхиальной астмой, экзогенным аллергическим альвеолитом) и нерационального назначения АБТ (редкое назначение ингибитор защищенных аминопенициллинов, цефуроксим аксетила, респираторных фторхинолонов, продолжение использования в терапевтической практике аминогликозидов, котримаксозола и тетрациклинов). По нашим наблюдениям, затяжному течению пневмонии способствовали курение и наличие выраженных посттуберкулезных изменений в легких, а также пожилой и старческий возраст пациентов. 18 больных неосложненной внебольничной пневмонией, в т. ч. с затяжным течением заболевания, обострением хронического бронхита и бронхоэктатической болезни, получили в отделении курс лечения спарфлоксацином, применение которого оказалось успешным у всех пациентов

Study of the background in the measuring station at the n_TOF facility at CERN: sources and solutions

A background roughly two orders of magnitude higher than tolerable was found in the n_TOF facility at CERN during the first measurements [1]. This note describes a series of additional measurements performed in the n_TOF experimental area to study the origin and the characteristics of the background. The program of these measurements was determined taking into account the results from the simulations carried out by the EET group [2]. A first phase of measurements confirmed what was expected from the simulations, namely that the dominant source of background was due to neutrons generated by negative muon capture. Actions to reduce the background were taken according to the results from both measurements and simulations. An iron shielding wall 3.2 m thick was then placed in between the sweeping magnet and the second collimator, with the purpose of stopping most of the muons. In a second phase of measurements, results showed that the additional shielding reduced the main component of the background by about a factor of 30