105 research outputs found
GM1 Ganglioside role in the interaction of Alpha-synuclein with lipid membranes: Morphology and structure
Alpha-Synuclein (AS) is the protein playing the major role in Parkinson's disease (PD), a neurological disorder characterized by the degeneration of dopaminergic neurons and the accumulation of AS into amyloid plaques. The aggregation of AS into intermediate aggregates, called oligomers, and their pathological relation with biological membranes are considered key steps in the development and progression of the disease. Here we propose a multi-technique approach to study the effects of AS in its monomeric and oligomeric forms on artificial lipid membranes containing GM1 ganglioside. GM1 is a component of functional membrane micro-domains, called lipid rafts, and has been demonstrated to bind AS in neurons. With the aim to understand the relation between gangliosides and AS, here we exploit the complementarity of microscopy (Atomic Force Microscopy) and neutron scattering (Small Angle Neutron Scattering and Neutron Reflectometry) techniques to analyze the structural changes of two different membranes (Phosphatidylcholine and Phosphatidylcholine/GM1) upon binding with AS. We observe the monomer- and oligomer-interactions are both limited to the external membrane leaflet and that the presence of ganglioside leads to a stronger interaction of the membranes and AS in its monomeric and oligomeric forms with a stronger aggressiveness in the latter. These results support the hypothesis of the critical role of lipid rafts not only in the biofunctioning of the protein, but even in the development and the progression of the Parkinson's disease
Qualidade física de um latossolo sob plantio direto influenciada pela cobertura do solo
Absorption of primary macronutrients and soybean growth at different compactation densities and moisture levels in a silt loam soil
Homogeneidade da qualidade física do solo nas entrelinhas de um pomar de laranjeira com sistemas de manejo da vegetação permanente
Compactação do solo e intervalo hídrico ótimo no crescimento e na produtividade da cultura do milho
Different fasting periods in tiletamine-zolezepam-anethetized cats: Glycemia, recovery, blood-gas and cardiorrespiratory parameters
Soil structure and greenhouse gas production differences between row and interrow positions under no-tillage
Qualidade física e intervalo hídrico ótimo em latossolo e cambissolo, cultivados com cafeeiro, sob manejo conservacionista do solo
Atributos físico-hídricos de um Latossolo Vermelho eutroférrico sob diferentes sistemas de manejo
Efeito da compactação do solo e do sulfentrazone sobre a cultura da soja em duas condições de água no solo
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