Genome-Wide Identification and Analysis of Grape Aldehyde Dehydrogenase (ALDH) Gene Superfamily

The completion of the grape genome sequencing project has paved the way for novel gene discovery and functional analysis. Aldehyde dehydrogenases (ALDHs) comprise a gene superfamily encoding NAD(P)(+)-dependent enzymes that catalyze the irreversible oxidation of a wide range of endogenous and exogenous aromatic and aliphatic aldehydes. Although ALDHs have been systematically investigated in several plant species including Arabidopsis and rice, our knowledge concerning the ALDH genes, their evolutionary relationship and expression patterns in grape has been limited.A total of 23 ALDH genes were identified in the grape genome and grouped into ten families according to the unified nomenclature system developed by the ALDH Gene Nomenclature Committee (AGNC). Members within the same grape ALDH families possess nearly identical exon-intron structures. Evolutionary analysis indicates that both segmental and tandem duplication events have contributed significantly to the expansion of grape ALDH genes. Phylogenetic analysis of ALDH protein sequences from seven plant species indicates that grape ALDHs are more closely related to those of Arabidopsis. In addition, synteny analysis between grape and Arabidopsis shows that homologs of a number of grape ALDHs are found in the corresponding syntenic blocks of Arabidopsis, suggesting that these genes arose before the speciation of the grape and Arabidopsis. Microarray gene expression analysis revealed large number of grape ALDH genes responsive to drought or salt stress. Furthermore, we found a number of ALDH genes showed significantly changed expressions in responses to infection with different pathogens and during grape berry development, suggesting novel roles of ALDH genes in plant-pathogen interactions and berry development.The genome-wide identification, evolutionary and expression analysis of grape ALDH genes should facilitate research in this gene family and provide new insights regarding their evolution history and functional roles in plant stress tolerance

Elaboration and characterization of acellular glass based culletloaded with carbon fibers

The foams glass whose porosity is very important, are counted among the new glass products that meet certain desired comfort needs in the particular building area (thermal and acoustic insulation). The objective of this study is to determine the influence of the carbon fibers addition influence on the porosity, the thermal insulation and dielectric loss of foams glass prepared by sintering at 800 °C, which opens the way to foam glass many possibilities for industrial to extend their opportunities that are very varied from thermal insulation to electromagnetic waves absorption. The carbon fibers addition used for this study is 1, 2, 3.5 and 5 wt% in relation to the foam glass load. Physico-chemical analysis techniques such as SEM-EDS, porosity, thermal insulation, differential thermal analysis, and dielectric tests have been used for the foams glass characterization. The obtained microstructure results clearly show that the 5 wt% carbon fibers addition increases the foam glass porosity which leads to low thermal conductivity (λ=0.0281 W/m°C) and increases its thermal insulating capacity. Furthermore the specific dielectric properties as real permittivity (ε'=2.7619) and high dielectric loss (tan δ=0.02969) favor the use of these glass filled foams with carbon fibers in the electromagnetic waves microwave absorption in the weakest working frequencies (mobile phones and Wi-Fi). © 2015 Elsevier B.V. All rights reserve

Elaboration and characterization of foam glass based waste Asian

The porous glass ‘foam glass’ is considered as the new glass products fulfill certain requirements in the building industry in particular (thermal and acoustic insulation). The production of foam glass based waste glass plays an important role in environmental protection and also gains in energy. As part of present work, we seek to improve the properties of glass to obtain a building material lighter with excellent insulation properties. The properties of foam glass depend on the porosity and morphology. The present work devoted to analyze the microstructure of the glass produced by scanning electron microscopy and optical microscopy to be more precise on the size and shape of pores constitute this materia

Nicotinamide cofactors suppress active-site labeling of aldehyde dehydrogenases

Active site labeling by (re)activity-based probes is a powerful chemical proteomic tool to globally map active sites in native proteomes without using substrates. Active site labeling is usually taken as a readout for the active state of the enzyme because labeling reflects the availability and reactivity of active sites, which are hallmarks for enzyme activities. Here, we show that this relationship holds tightly, but we also reveal an important exception to this rule. Labeling of Arabidopsis ALDH3H1 with a chloroacetamide probe occurs at the catalytic Cys, and labeling is suppressed upon nitrosylation and oxidation, and upon treatment with other Cys modifiers. These experiments display a consistent and strong correlation between active site labeling and enzymatic activity. Surprisingly, however, labeling is suppressed by the cofactor NAD(+), and this property is shared with other members of the ALDH superfamily and also detected for unrelated GAPDH enzymes with an unrelated hydantoin-based probe in crude extracts of plant cell cultures. Suppression requires cofactor binding to its binding pocket. Labeling is also suppressed by ALDH modulators that bind at the substrate entrance tunnel, confirming that labeling occurs through the substrate-binding cavity. Our data indicate that cofactor binding adjusts the catalytic Cys into a conformation that reduces the reactivity toward chloroacetamide probes

Synthesis and evaluation of the pharmacological activity of open analogues of cromakalim carrying urea and thiourea moieties

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