15 research outputs found
Graphical representation of CL and SL of some cultivars.
<p>Graphical representation of CL and SL of some cultivars.</p
Diagrammatic representation of internodal elongation pattern in different gene combinations.
<p>Diagrammatic representation of internodal elongation pattern in different gene combinations.</p
Effect of <i>Rht1</i> and <i>Rht8</i> on different agronomic traits.
<p>Effect of <i>Rht1</i> and <i>Rht8</i> on different agronomic traits.</p
Percent decrease in CL and SL in each genotypic category.
<p>Percent decrease in CL and SL in each genotypic category.</p
Effect of <i>Rht1</i> and <i>Rht8</i> on seedling traits (CL and SL).
<p>Effect of <i>Rht1</i> and <i>Rht8</i> on seedling traits (CL and SL).</p
Evaluation of 19,460 Wheat Accessions Conserved in the Indian National Genebank to Identify New Sources of Resistance to Rust and Spot Blotch Diseases
<div><p>A comprehensive germplasm evaluation study of wheat accessions conserved in the Indian National Genebank was conducted to identify sources of rust and spot blotch resistance. Genebank accessions comprising three species of wheat–<i>Triticum aestivum</i>, <i>T</i>. <i>durum</i> and <i>T</i>. <i>dicoccum</i> were screened sequentially at multiple disease hotspots, during the 2011–14 crop seasons, carrying only resistant accessions to the next step of evaluation. Wheat accessions which were found to be resistant in the field were then assayed for seedling resistance and profiled using molecular markers. In the primary evaluation, 19,460 accessions were screened at Wellington (Tamil Nadu), a hotspot for wheat rusts. We identified 4925 accessions to be resistant and these were further evaluated at Gurdaspur (Punjab), a hotspot for stripe rust and at Cooch Behar (West Bengal), a hotspot for spot blotch. The second round evaluation identified 498 accessions potentially resistant to multiple rusts and 868 accessions potentially resistant to spot blotch. Evaluation of rust resistant accessions for seedling resistance against seven virulent pathotypes of three rusts under artificial epiphytotic conditions identified 137 accessions potentially resistant to multiple rusts. Molecular analysis to identify different combinations of genetic loci imparting resistance to leaf rust, stem rust, stripe rust and spot blotch using linked molecular markers, identified 45 wheat accessions containing known resistance genes against all three rusts as well as a QTL for spot blotch resistance. The resistant germplasm accessions, particularly against stripe rust, identified in this study can be excellent potential candidates to be employed for breeding resistance into the background of high yielding wheat cultivars through conventional or molecular breeding approaches, and are expected to contribute toward food security at national and global levels.</p></div
Seedling resistance in wheat accessions.
<p>These genebank accessions were identified as resistant in the primary field evaluation at the hotspots. The seedling resistance was recorded as resistant either to only one rust disease (leaf, stem or stripe) or a combination of two or all the three rust diseases. Seedling resistance screening was carried out under controlled condition at the Regional Station of Indian Institute of Wheat and Barley Research (IIWBR), Flowerdale, Shimla. Accession-wise details are provided in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0167702#pone.0167702.s003" target="_blank">S3 Table</a>.</p
Amplicon profile of 137 wheat accessions for SSR marker G<i>wm427</i> linked to <i>Sr13</i>. M = 100bp ladder.
<p>Amplicon profile of 137 wheat accessions for SSR marker G<i>wm427</i> linked to <i>Sr13</i>. M = 100bp ladder.</p
Locations of evaluation experiments of wheat germplasm against rusts and spot blotch.
<p>Primary screening against the three rusts was carried out at Wellington. Subsequent screening for stripe rust resistance was done at Gurdaspur and for spot blotch resistance at Cooch Behar. The seedling resistance assay was carried out at Flowerdale and molecular profiling was done at New Delhi. Base map was generated using DIVA-GIS data (<a href="http://www.diva-gis.org/gdata" target="_blank">www.diva-gis.org/gdata</a>).</p