The effects of transparency on perceived and actual competence of a content-based recommender.

Perceptions of a system’s competence influence acceptance of that system [31]. Ideally, users’ perception of competence matches the actual competence of a system. This paper investigates the relation between actual and perceived competence of transparent Semantic Web recommender systems that explain recommendations in terms of shared item concepts. We report an experiment comparing non-transparent and transparent versions of a content-based recommender. Results indicate that in the transparent condition, perceived competence and actual competence (in specific recall) were related, while in the non-transparent condition they were not. Providing insight in what aspects of items triggered their recommendation, by showing the concepts that were the basis for a recommendation, gave users a better assessment of how well the system worked

The development of burfi with date paste

Burfi with date paste was prepared by mixing the date paste with khoa as per standard method. Date paste was added at 10%, 20%, 30% and 40% levels to the weight of khoa. Burfi prepared with addition of date paste up to 30% level was best accepted and resembled the control in sensory attributes. Addition of date paste into burfi changed the composition of burfi. The moisture, total solids, ash, lactose, fat, protein and HMF changed significantly however, FFA remained unchanged in final product when compared to control sample. Addition of date paste into burfi significantly increased the acidity and lowered the pH of burfi. The production cost of burfi with date paste was slightly higher than control burfi due to additional cost of date paste

Two integrated and highly predictive functional analysis-based procedures for the classification of MSH6 variants in Lynch syndrome

Purpose: Variants in the DNA mismatch repair (MMR) gene MSH6, identified in individuals suspected of Lynch syndrome, are difficult to classify owing to the low cancer penetrance of defects in that gene. This not only obfuscates personalized health care but also the development of a rapid and reliable classification procedure that does not require clinical data. Methods: The complete in vitro MMR activity (CIMRA) assay was calibrated against clinically classified MSH6 variants and, employing Bayes’ rule, integrated with computational predictions of pathogenicity. To enable the validation of this two-component classification procedure we have employed a genetic screen to generate a large set of inactivating Msh6 variants, as proxies for pathogenic variants. Results: The genetic screen-derived variants established that the two-component classification procedure displays high sensitivities and specificities. Moreover, these inactivating variants enabled the direct reclassification of human variants of uncertain significance (VUS) as (likely) pathogenic. Conclusion: The two-component classification procedure and the genetic screens provide complementary approaches to rapidly and cost-effectively classify the large majority of human MSH6 variants. The approach followed here provides a template for the classification of variants in other disease-predisposing genes, facilitating the translation of personalized genomics into personalized health care

Third generation cephalosporin use in a tertiary hospital in Port of Spain, Trinidad: need for an antibiotic policy

BACKGROUND: Tertiary care hospitals are a potential source for development and spread of bacterial resistance being in the loop to receive outpatients and referrals from community nursing homes and hospitals. The liberal use of third-generation cephalosporins (3GCs) in these hospitals has been associated with the emergence of extended-spectrum beta- lactamases (ESBLs) presenting concerns for bacterial resistance in therapeutics. We studied the 3GC utilization in a tertiary care teaching hospital, in warded patients (medical, surgical, gynaecology, orthopedic) prescribed these drugs. METHODS: Clinical data of patients (≥ 13 years) admitted to the General Hospital, Port of Spain (POSGH) from January to June 2000, and who had received 3GCs based on the Pharmacy records were studied. The Sanford Antibiotic Guide 2000, was used to determine appropriateness of therapy. The agency which procures drugs for the Ministry of Health supplied the cost of drugs. RESULTS: The prevalence rate of use of 3GCs was 9.5 per 1000 admissions and was higher in surgical and gynecological admissions (21/1000) compared with medical and orthopedic (8 /1000) services (p < 0.05). Ceftriaxone was the most frequently used 3GC. Sixty-nine (36%) patients without clinical evidence of infection received 3Gcs and prescribing was based on therapeutic recommendations in 4% of patients. At least 62% of all prescriptions were inappropriate with significant associations for patients from gynaecology (p < 0.003), empirical prescribing (p < 0.48), patients with undetermined infection sites (p < 0.007), and for single drug use compared with multiple antibiotics (p < 0.001). Treatment was twice as costly when prescribing was inappropriate CONCLUSIONS: There is extensive inappropriate 3GC utilization in tertiary care in Trinidad. We recommend hospital laboratories undertake continuous surveillance of antibiotic resistance patterns so that appropriate changes in prescribing guidelines can be developed and implemented. Though guidelines for rational antibiotic use were developed they have not been re-visited or encouraged, suggesting urgent antibiotic review of the hospital formulary and instituting an infection control team. Monitoring antibiotic use with microbiology laboratory support can promote rational drug utilization, cut costs, halt inappropriate 3GC prescribing, and delay the emergence of resistant organisms. An ongoing antibiotic peer audit is suggested

Highly Sensitive Colorimetric Biosensor for Staphylococcal Enterotoxin B by a Label-Free Aptamer and Gold Nanoparticles

A simple, sensitive and selective colorimetric biosensor for the detection of Staphylococcal enterotoxin B (SEB) was developed using SEB-binding aptamer (SEB2) as recognition element and unmodified gold nanoparticles (AuNPs) as colorimetric probes. The assay is based on color change from red to purple due to conformational change of aptamer in the presence of SEB, and the phenomenon of salt-induced AuNPs aggregation which could be monitored by naked eye or UV–vis spectrometer. Results showed that the AuNPs can effectively differentiate the SEB induced conformational change of the aptamer in the presence of a given high salt concentration. A linear response in the range of 50 μg/mL to 0.5 ng/mL of SEB concentration was obtained. The assay was highly specific to SEB as compared to other related toxins. The limit of detection (LOD) of SEB achieved within few minutes was 50 ng/mL visually and spectrometric method improved it to 0.5 ng/mL. Robustness of the assay was tested in artificially spiked milk samples and cross-checked using in house developed sandwich ELISA (IgY as capturing and SEB specific monoclonal as revealing antibody) and PCR. This colorimetric assay could be a suitable alternative over existing methods during biological emergencies due to its simplicity, sensitive and cost effectiveness

Colorimetric DNAzyme Biosensor for Convenience Detection of Enterotoxin B Harboring <i>Staphylococcus aureus</i> from Food Samples

In the present study, a colorimetric DNAzymes biosensor strategy was devised in combination with immunomagnetic separation for rapid and easy detection of enterotoxin B harboring <i>Staphylococcus aureus</i> from food and clinical samples. The method employs immunocapture of <i>S. aureus</i> and amplification of <i>seb</i> gene by DNAzyme complementary sequence integrated forward primer and with specific reverse primer. The DNAzyme sequence integrated dsDNA PCR products when treated with hemin and TMB (3,3′,5,5′-tetramethylbenzidine) in the presence of H₂O₂ produce colorimetric signal. A linear relationship of optical signal with the initial template of <i>seb</i> was obtained which could be monitored by visually or spectrophotrometrically for qualitative and quantitative detection. The limit of detection for the assay was approximately 10² CFU/mL of <i>seb</i> gene harboring target. This method is convenient compared to gel based and ELISA systems. Further, spiking studies and analysis on natural samples emphasized the robustness and applicability of developed method. Altogether, the established assay could be a reliable alternative, low-cost, viable detection tool for the routine investigation of <i>seb</i> from food and clinical sources

The effects of transparency on perceived and actual competence of a content-based recommender

Perceptions of a system's competence influence acceptance of that system [31]. Ideally, users' perception of competence matches the actual competence of a system. This paper investigates the relation between actual and perceived competence of transparent Semantic Web recommender systems that explain recommendations in terms of shared item concepts. We report an experiment comparing non-transparent and transparent versions of a content-based recommender. Results indicate that in the transparent condition, perceived competence and actual competence (in specific recall) were related, while in the non-transparent condition they were not. Providing insight in what aspects of items triggered their recommendation, by showing the concepts that were the basis for a recommendation, gave users a better assessment of how well the system worked. Keywords: Actual competence; Explanations; Perceived competence; Recommender systems; Transparenc