POJAVA CEREBROVASKULARNOG INZULTA U OVISNOSTI O TIPOVIMA VREMENA I FRONTAMA

U radu je analizirana uvjetna vjerojatnost pojavljivanja cerebrovaskularnih inzulta kad se pojavio određeni tip vremena, kao i učestalost cerebrovaskularnih inzulta u 7-dnevnom razdoblju n ± 3 dana oko centralno smještenog dana s prolazom hladne ili tople fronte. Pokazalo se je da su cerebrovaskularni udari najčešći u tipovima vremena s niskim tlakom zraka (doline i ciklone) te u situacijama kad nad naše područje iz velikih udaljenosti dolaze zračne mase bitno drugačijih karakteristika od prevladavajućih - zimi N, NE tipovi, a u proljeće i jesen S, SW tipovi vremena. Učestalost CVI veća je u dane s hladnom frontom, a zimi i u proljeće i 1-2 dana uoči njega, a ljeti dan nakon prolaza hladne fronte

Early Graft Loss after Kidney Transplantation: Endothelial Dysfunction of Renal Microvasculature

Decision process about the acceptance of the deceased donor kidney for transplantation might be challenging. Although histological evaluation of pretransplant donor kidney biopsy provides reliable information regarding cortical necrosis, vascular thrombosis, extensive global glomerulosclerosis, and interstitial fibrosis/tubular atrophy, only electron microscopy enables thorough and reliable insights into microvasculature changes of kidney graft. The aim of the present paper is to briefly present two cases of early kidney graft loss. In one case, the donor was exposed to long-term extracorporeal membrane oxygenation (ECMO); in the other case, the donor experienced Takotsubo cardiomyopathy. In both cases, light microscopy of pretransplant biopsy found no pathology or significant discrepancy in morphology of kidney graft, while electron microscopy revealed severe endothelial dysfunction of renal microvasculature. Our results suggest that severe injury of renal microvasculature with relatively preserved tubular epithelium may be associated with some conditions of deceased kidney donors leading to early kidney graft nonfunction and loss. Further studies are needed to determine prognostic significance of severe ultrastructural microvasculature lesions and to evaluate disease states and conditions that could be associated with severe endothelial dysfunction of kidney graft

Molecular characterization of the coat protein gene of prunus necrotic ringspot virus infecting peach in Montenegro

In 2011 and 2012, two major peach-growing areas in Montenegro were surveyed for the occurrence of eleven peach viruses. Samples collected from 105 peach and nectarine trees were tested by Reverse transcription-polymerase chain reaction (RT-PCR) using sets of primers specific for the detection of Apple chlorotic leaf spot virus (ACLSV), Apple mosaic virus (ApMV), Cherry mottle leaf virus (CMLV), Prunus necrotic ringspot virus (PNRSV), Prune dwarf virus (PDV), Peach mosaic virus (PMV), Peach rosette mosaic virus (PRMV), Tobacco ringspot virus (TRSV) and Tomato ringspot virus (ToRSV). The occurrence of Strawberry latent ringspot virus (SLRSV) was examined using nested RT-PCR, while a Real-time quantitative RT-PCR method was applied for detection of Plum pox virus (PPV). PPV, PNRSV and PDV were detected in 42.9, 24.8 and 0.9 % of samples. Mixed viral infections were recorded in 6.7 % of samples, while results showed the absence of ACLSV, ApMV, PMV, CMLV, SLRSV, PRMV, TRSV and ToRSV from all assayed samples. In particular, the PCR products of complete coat protein (CP) gene was cloned and sequenced from fourteen PNRSV isolates. A high identity (87.5–100 %) was found between CP nucleotide sequences of Montenegrin isolates and other PNRSV isolates from different hosts and geographic regions retrieved from GenBank. CP nucleotide sequence comparison, as well as phylogenetic reconstruction confirmed the clustering of the isolates into three molecular groups (PV-96, PV-32 and PE-5), whereby Montenegrin isolates were separated into two phylogroups (PV-96 and PE-5). Comparative sequence and phylogenetic analysis showed that clustering of various isolates was not associated with geographic and host origin

Direct evidence for W exchange in charmed meson decay

Using the ARGUS detector at DORIS II, we have observed a signal of 36.7±8.0 events in the decay channel D 0 →K s 0 φ . In the same data sample, we have observed the well established decay D 0 →K s 0 π + π − , and find the ratio, Br(D) 0 → K s 0 φ) Br(D) 0 → K s 0 π + π − ) , to be 0.186±0.052. The substantial value of (0.99±0.32±0.17)% then derived for the branching ratio for D 0 → K 0 φ gives direct evidence that W exchange contributes D 0 decay