DEVELOPMENT OF A METHODOLOGY FOR ASSESSING THE VIABILITY OF MICROORGANISMS BY THE TURBIDIMETRIC METHOD

Turbidimetry - quantitative analysis of the composition and properties of substances, based on measuring the amount of light absorbed by a suspension. In our study, an attempt was made to create a method for assessing the viability of microorganisms by the turbidimetric method

Outpatient technologies in policlinic department of regional oncology center

Ап experience of our day hospital at the polyclinic GBUZ CHOKOD that allows to solve the problem to improve the organization of specialized medical care, an increase in work intensity outpatient care clinic, increasing the availability and quality of specialized medical care.Приведен наш опыт организации дневного стационара при поликлинике ГБУЗ ЧОКОД, позволяющий решить задачу по совершенствованию организации специализированной медицинской помощи, увеличению интенсивности работы амбулаторно-поликлинических звена диспансера, повышению доступности и качества специализированной медицинской помощи

Functional activity of blood eosinophils in patients with <i>Opisthorchis felineus</i> invasion

Aim. To study the functional activity of blood eosinophils in patients with Opisthorchis felineus (O. felineus) invasion.Material and methods. A total of 328 patients with O. felineus invasion (120 men and 208 women, mean age 40.3 years) and 34 practically healthy patients (14 men and 20 women, mean age 41.1 years) aged 18 to 70 years were examined. The main method for diagnosing opisthorchiasis was the determination of eggs or bodies of adult parasites in the duodenal contents and/or feces, which was used in all 328 patients with opisthorchiasis. All patients underwent a complete blood count and biochemical blood test, esophagogastroduodenoscopy and ultrasound examination of the abdominal organs. The study of the functional activity of blood eosinophils was carried out in 42 patients with opisthorchiasis and 34 healthy individuals from the control group by chemiluminescent analysis with the measurement of the intensity of production of reactive oxygen species in spontaneous and zymosan-induced reactions in lucigenin- and luminol-dependent processes.Results. The frequency of eosinophils in the complete blood count above 5% in the examined patients with opisthorchiasis was 19.5%. An increase in the content of eosinophils in the blood in patients with opisthorchiasis was associated with an increase in the frequency of complaints of pain in the right hypochondrium, pruritus, skin rashes, an increase in the proportion of leukopenia and neutropenia, an increase in the level of gamma-glutamyl transpeptidase in the blood, and an increase in the frequency of ultrasound signs of cholecystitis. At the same time, a pronounced increase in the functional activity of blood eosinophils, determined by the chemiluminescent method, was observed in patients with O. felineus invasion.Conclusions. Patients with O. felineus invasion with an increase in the content of eosinophils in the blood due to the likelihood of an aggressive course of the pathology deserve the close attention of practitioners with the need for a thorough comprehensive examination of patients, including immunological methods

Colloidal ribbons and rings from Janus magnetic rods

Dipolar particles are fundamental building blocks in nature and technology, yet the effect of particle anisotropy is seldom explored. Here, we fabricate colloidal silica rods coated with a hemicylindrical magnetic layer to satisfy multiple criteria: nearly monodisperse, easily imaged and magnetic interaction that dominates over gravity. We confirm long-predicted features of dipolar assembly and stress the microstructural variety brought about by shape and constituent anisotropy, especially by extrapolating knowledge learned from literal molecules. In this colloidal system, we describe analogies to liquid crystalline deformations with bend, splay and twist; an analogy to cis/trans isomerism in organic molecules, which in our system can be controllably and reversibly switched; and a field-switching methodology to direct single ribbons into not only single but also multiple rings that can subsequently undergo hierarchical self-assembly. We highlight subtle material issues of control and design rules for reconfigurable dipolar materials with building blocks of complex shape.close221