Gene editing (CRISPR-Cas) technology and fisheries sector

Considering the advantages of gene editing technologies, in recent years, emphasis has been given on three main techniques of gene editing i.e. zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and the CRISPR/Cas9 RNA-guided endonuclease system. In all of these three technologies one thing is common i.e. the technology utilizes restriction enzymes to break down the double stranded DNA molecule at a targeted location with the help of another molecule of homologous binding protein or RNA, which is also called as a guide RNA molecule. This targeted breaking and repair of the DNA molecule as per our requirement is generally viewed as a great breakthrough in gene therapy methods. In fisheries sector, in order to promote aquaculture/mariculture activities, aquaculture industries are facing number of challenges, particularly in area of quality and demand of seed production, control of health and disease management, production of quality traits with phenotypically improved varieties, and strengthening of immune system. Several efforts are being made both at public and private sectors to develop scientific technologies to meet these challenges and substantial achievements have also been made. But still these challenges remained as major constraints in hampering the growth of this industry as per the demand and expectations. However, with the advent of now recently developed gene editing techniques, we may have to explore and evaluate the possibilities of applications of this technology as a long lasting solution in addressing at least some of the vital issues of the aquaculture industry particularly in those related to altering targeted gene structure in the species for positive impact

Molecular and Cytogenitic characterization of fish cell lines and its application in aquatic research

Fish cell line has emerged as an important tool in fishery biotechnology. In recent years, various fish cell lines have been developed by different researchers across the country. National Repository on Fish cell lines, established with the aim to preserve fish cell lines for training and education to stakeholders, has started functioning at National Bureau of Fish Genetic Resources, Lucknow. This repository is supposed to characterize and preserve the fish lines developed across the country and serve as a national referral centre for Indian and exotic fish cell lines. Currently, the repository is maintaining 50 fish cell lines deposited by various research institutes in India, including the cell lines developed at cell culture facility of National Bureau of Fish Genetic Resources. The cell lines have been successfully cryopreserved after verifying its authenticity by sequence analysis of two mitochondrial genes, viz.16S rRNA and cytochrome c oxidase sub-unit I. Chromosomal analysis, transfection efficiency and immunocytochemistry are also being used to characterize the cell lines. The facility is serviceable for the collection, deposition and distribution of fish cell lines. This paper discusses the status as well as the methodology adopted for fish cell lines development, characterization and storage at NRFC

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Not AvailableEmployment generation in tribal areas through aquaculture technologies and fishery estatesNot Availabl

Viscous flow computations using a meshless solver, LSFD-U

The Upwind-Least Squares Finite Difference (LSFD-U) scheme has been successfully applied for inviscid flow computations. In the present work, we extend the procedure for computing viscous flows. Different ways of discretizing the viscous fluxes are analysed for the positivity, which determines the robustness of the solution procedure. The scheme which is found to be more positive is employed for viscous flux computation. The numerical results for validating the procedure are presented

Employment generation in tribal areas through aquaculture technologies and fishery estates

Employment generation in tribal areas through aquaculture technologies and fishery estate

Not Available

Not AvailableConsidering the advantages of gene editing technologies, in recent years, emphasis has been given on three main techniques of gene editing i.e. zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and the CRISPR/Cas9 RNA-guided endonuclease system. In all of these three technologies one thing is common i.e. the technology utilizes restriction enzymes to break down the double stranded DNA molecule at a targeted location with the help of another molecule of homologous binding protein or RNA, which is also called as a guide RNA molecule. This targeted breaking and repair of the DNA molecule as per our requirement is generally viewed as a great breakthrough in gene therapy methods. In fisheries sector, in order to promote aquaculture/mariculture activities, aquaculture industries are facing number of challenges, particularly in area of quality and demand of seed production, control of health and disease management, production of quality traits with phenotypically improved varieties, and strengthening of immune system. Several efforts are being made both at public and private sectors to develop scientific technologies to meet these challenges and substantial achievements have also been made. But still these challenges remained as major constraints in hampering the growth of this industry as per the demand and expectations. However, with the advent of now recently developed gene editing techniques, we may have to explore and evaluate the possibilities of applications of this technology as a long lasting solution in addressing at least some of the vital issues of the aquaculture industry particularly in those related to altering targeted gene structure in the species for positive impact.Not Availabl