123 research outputs found
Polymorphism of genes associated with infectious lung diseases in Northern Asian populations and in patients with community-acquired pneumonia
The innate IΠ½Π½\mmune system is the first to respond to invading pathogens. It is responsible for invader recognition, immune-cell recruitment, adaptive-immunity activation, and regulation of inflammation intensity. Previously, two single-nucleotide polymorphisms of innate-immunity genes β rs5743708 (Arg753Gln) of the TLR2 gene and rs8177374 (Ser180Leu) of the TIRAP gene β have been shown to be associated with both pneumonia and tuberculosis in humans, but the data are contradictory among different ethnic groups. It has also been reported that rs10902158 at the PKP3-SIGGIR-TMEM16J genetic locus belongs to a haplotype race-specifically associated with tuberculosis. Meanwhile, a gradient of its frequency is observed in Asia. The aim of this work was to assess the effect of selection for the genotypes of the above-mentioned SNPs on the gene pools of populations living in harsh climatic conditions that contribute to the development of infectious lung diseases. We estimated the prevalence of these variants in white and Asian (Chukchis and Yakuts) population samples from Northern Asia and among patients with community-acquired pneumonia (CAP). Carriage of the rs5743708 A allele was found to predispose to severe CAP (odds ratio 2.77, p = 0.021), whereas the GG/CT genotype of rs5743708/rs8177374 proved to be protective against it (odds ratioΒ 0.478, p = 0.022) in white patients. No association of rs10902158 with CAP (total or severe) was found among whites. Stratification of CAP by causative pathogen may help eliminate the current discrepancies between different studies. No significant difference in rs5743708 or rs8177374 was found between adolescent and long-lived white samples. Carriage of the alleles studied is probably not associated with predisposition to longevity among whites in Siberia. Both white and Asian populations studied were different from Western European and East Asian populations in the variantsβ prevalence. The frequency of the rs8177374 T (Ser180Leu) variant was significantly higher in the Chukchi sample (p = 0, Ο2 = 63.22) relative to the East Asian populations. This result may confirm the hypothesis about the selection of this allele in the course of human migration into areas with unfavorable climatic conditions
Meteoprotective properties of melaxen in old and middle aged patients with ischemic heart disease in combination with arterial hypertension
We studied 102 patients (mean age 60.1Β±3.3 years) with arterial hypertension (AH) II-III stage, grade 2-3 and ischemic heart disease (exertional angina functional class (FC) I-II, postinfarction cardiosclerosis). The control group of patients received traditional therapy (TT): beta -blockers, calcium channel blockers, ACE inhibitors, antiplatelet agents, diuretics and nitrate
Detection of Five New RRATs at 111 MHz
Results of 111-MHz monitoring observations carried out on the Big Scanning
Antenna of the Pushchino Radio Astronomy Observatory during September 1-28,
2015 are presented. Fifty-four pulsating sources were detected at declinations
. Forty-seven of these are known pulsars, five are new
sources, and two are previously discovered transients. Estimates of the peak
flux densities and dispersion measures are presented or all these sources.Comment: published in Astronomy Report, translated by Yandex translator with
correction of scientific lexis, 8 pages, 2 figures, 3 table
Knot homology via derived categories of coherent sheaves II, sl(m) case
Using derived categories of equivariant coherent sheaves we construct a knot
homology theory which categorifies the quantum sl(m) knot polynomial. Our knot
homology naturally satisfies the categorified MOY relations and is
conjecturally isomorphic to Khovanov-Rozansky homology. Our construction is
motivated by the geometric Satake correspondence and is related to Manolescu's
by homological mirror symmetry.Comment: 51 pages, 9 figure
Associations of changes in lipid metabolism parameters and the severity of COVID-19 infection in Novosibirsk residents
Aim. To study the associations of changes in lipid metabolism parameters and the severity of a coronavirus disease 2019 (COVID-19).Material and methods. This cross-sectional study included 270 patients aged 26-84 years (mean age, 53,09Β±13,22 years) who had COVID-19 within prior two months, which were divided into 3 groups: mild (1, n=128), moderate (2, n=128) and severe (3, n=14) COVID-19. Patients were assessed for body mass index (BMI), waist circumference (WC), hip circumference (HC), waist-to-hip ratio (WHR), total cholesterol, triglycerides (TG), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C). In addition, atherogenic index of plasma (AIP) was calculated. Statistical processing was performed using the SPSS software package (version 13.0).Results. Patients with severe COVID-19 had significantly higher levels of TG and AIP compared with patients with moderate and mild course. BMI and WC were significantly higher in patients in groups 2 and 3 compared with patients in group 1. In women, BMI and AIP levels were significantly higher in the severe COVID-19 group compared to groups 1 and 2. HDL-C levels were lower in patients with severe COVID-19 compared to those with moderate disease. WHR was higher among men in group 3 compared with group 1.Conclusion. Patients with severe COVID-19 have higher BMI, WC, AIP, TG levels, and lower HD-C levels. The relative odds for severe COVID-19 are associated with increased WC, AIP, TG, and lower HDL-C
Non-alcoholic fatty liver disease and metabolic liver dysfunction in the new coronavirus infection COVID-19
Our study aimed to explore associated non-alcoholic fatty liver disease (NAFLD) and metabolic liver dysfunction influence on the severity of the new coronavirus infection COVID-19. Material and methods. The study design was a cross-sectional study. The research included 215 patients (39.50 % of men) aged 26β60 years who had undergone a new coronavirus infection COVID-19 at least two months ago. Participants were divided into three groups by severity of infection: mild (n = 99), moderate and severe (n = 116) by anamnesis. Hepatic steatosis index (HIS), body mass index (BMI), waist circumference, alanine aminotransferase and gamma-glutamyl transpeptidase activity, glucose and triglyceride content, systolic and diastolic pressure were calculated and abdominal ultrasound examination was done. Results. In the group with moderate and severe course of COVID-19, the proportion of patients diagnosed with NAFLDaccording to the HSI index was significantly higher compared to patients with mild coronavirus infection. Patients with mild COVID-19, who were diagnosed with NAFLD, had higher alanine aminotransferase and gamma-glutamyl transpeptidase activity, glucose and triglyceride content, BMI, systolic and diastolic pressure, waist circumference compared to patients without NAFLD. Similar differences persisted for patients with moderate and severe course. With the step-by-step exclusion of cardiometabolic parameters from the logistic regression model, the triglyceride content and BMI retained association with steatohepatosis according to ultrasound data, regardless of severity. When creating a similar model for the HSI index, significant correlation was shown for alanine aminotransferase activity in patients with mild COVID-19, for alanine aminotransferase activity and BMI β in patients with moderate and severe COVID-19. Conclusions. Patients with NAFLD have a more severe course of COVID-19. In addition, associations of the severity of COVID-19 with a combination of NAFLD and other cardiometabolic changes in the body, such as arterial hypertension, obesity, dyslipidemia, were revealed
Π‘ΠΎΠ²ΡΠ΅ΠΌΠ΅Π½Π½ΡΠ΅ ΠΏΠΎΠ΄Ρ ΠΎΠ΄Ρ ΠΊ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΡ Π³Π»ΡΠΊΠΎΠΊΠΎΡΡΠΈΠΊΠΎΠΈΠ΄Π½ΡΡ ΠΈ ΡΠΈΡΠΎΡΠΎΠΊΡΠΈΡΠ΅ΡΠΊΠΈΡ ΠΏΡΠ΅ΠΏΠ°ΡΠ°ΡΠΎΠ² ΠΏΡΠΈ Π±ΠΎΠ»Π΅Π·Π½ΠΈ Π¨Π΅Π³ΡΠ΅Π½Π°
Tsel' issledovaniya. Otsenit' effektivnost' dlitel'noy terapii bolezni Shegrena (BSh) malymi dozami glyukokortikoidnykh (GK) i alkiliruyushchikh tsitotoksicheskikh (ATs) preparatov
Materialy i metody. V issledovanie vklyucheno 412 patsientov (406 zhenshchin i 6 muzhchin) s BSh, nablyudavshikhsya v Institute revmatologii v 1975 - 2007 gg. Mediana nablyudeniya - 9 (5-15) let. Sredniy vozrast bol'nykh na nachalo nablyudeniya - 44,8 goda. Mediana dlitel'nosti BSh do pervogo postupleniya - 9 let, mediana vozrasta debyuta BSh - 33 goda. Effektivnost' dlitel'noy terapii malymi dozami GKu 151 bol'nogo (mediana - 10 let), malymi dozami ATsu 147 bol'nykh (mediana - 5 let) otsenena v sravnenii s rezul'tatami lecheniya 94 bol'nykh, poluchavshikh tol'ko Β«oftal'mologicheskieΒ» i Β«stomatologicheskieΒ» metody terapii. Rezul'taty issledovaniya. Pokazano, chto terapiya malymi dozami GK i ATs polozhitel'no vliyala na nekotorye zhelezistye i vnezhelezistye proyavleniya zabolevaniya, odnako k snizheniyu veroyatnosti razvitiya limfoproliferativnykh zabolevaniy (r=0,036) i povysheniyu vyzhivaemosti bol'nykh s BSh privodila tol'ko terapiya ATs (r=0,037). Razrabotany i predstavleny metodiki lokal'nogo ispol'zovaniya GK pri tyazhelykh oftal'mologicheskikh i stomatologicheskikh proyavleniyakh BSh. U 150 bol'nykh s BSh s tyazhelymi sistemnymi proyavleniyami predstavleny pokazaniya i protivopokazaniya dlya provedeniya pul's-terapii (PT), kombinirovannoy PT, plazmafereza, krioafereza i dvoynoy fil'tratsii plazmy v sochetanii s kombinirovannoy PT. Pokazano, chto naznachenie malykh doz GK i PT u zhenshchin s BSh s sokhranennym menstrual'nym tsiklom privodit k bolee rannemu nastupleniyu menopauzy i sposobstvuet razvitiyu glyukokortikoidnogo i postmenopauzal'nogo osteoporoza v otdalennye sroki. Otseneny naibolee znachimye pobochnye proyavleniya terapii GK i ATs pri razlichnykh variantakh terapii BSh.Π¦Π΅Π»Ρ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ. ΠΡΠ΅Π½ΠΈΡΡ ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΠΎΡΡΡ Π΄Π»ΠΈΡΠ΅Π»ΡΠ½ΠΎΠΉ ΡΠ΅ΡΠ°ΠΏΠΈΠΈ Π±ΠΎΠ»Π΅Π·Π½ΠΈ Π¨Π΅Π³ΡΠ΅Π½Π° (ΠΠ¨) ΠΌΠ°Π»ΡΠΌΠΈ Π΄ΠΎΠ·Π°ΠΌΠΈ Π³Π»ΡΠΊΠΎΠΊΠΎΡΡΠΈΠΊΠΎΠΈΠ΄Π½ΡΡ
(ΠΠ) ΠΈ Π°Π»ΠΊΠΈΠ»ΠΈΡΡΡΡΠΈΡ
ΡΠΈΡΠΎΡΠΎΠΊΡΠΈΡΠ΅ΡΠΊΠΈΡ
(ΠΠ¦) ΠΏΡΠ΅ΠΏΠ°ΡΠ°ΡΠΎΠ²
ΠΠ°ΡΠ΅ΡΠΈΠ°Π»Ρ ΠΈ ΠΌΠ΅ΡΠΎΠ΄Ρ. Π ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠ΅ Π²ΠΊΠ»ΡΡΠ΅Π½ΠΎ 412 ΠΏΠ°ΡΠΈΠ΅Π½ΡΠΎΠ² (406 ΠΆΠ΅Π½ΡΠΈΠ½ ΠΈ 6 ΠΌΡΠΆΡΠΈΠ½) Ρ ΠΠ¨, Π½Π°Π±Π»ΡΠ΄Π°Π²ΡΠΈΡ
ΡΡ Π² ΠΠ½ΡΡΠΈΡΡΡΠ΅ ΡΠ΅Π²ΠΌΠ°ΡΠΎΠ»ΠΎΠ³ΠΈΠΈ Π² 1975 - 2007 Π³Π³. ΠΠ΅Π΄ΠΈΠ°Π½Π° Π½Π°Π±Π»ΡΠ΄Π΅Π½ΠΈΡ - 9 (5-15) Π»Π΅Ρ. Π‘ΡΠ΅Π΄Π½ΠΈΠΉ Π²ΠΎΠ·ΡΠ°ΡΡ Π±ΠΎΠ»ΡΠ½ΡΡ
Π½Π° Π½Π°ΡΠ°Π»ΠΎ Π½Π°Π±Π»ΡΠ΄Π΅Π½ΠΈΡ - 44,8 Π³ΠΎΠ΄Π°. ΠΠ΅Π΄ΠΈΠ°Π½Π° Π΄Π»ΠΈΡΠ΅Π»ΡΠ½ΠΎΡΡΠΈ ΠΠ¨ Π΄ΠΎ ΠΏΠ΅ΡΠ²ΠΎΠ³ΠΎ ΠΏΠΎΡΡΡΠΏΠ»Π΅Π½ΠΈΡ - 9 Π»Π΅Ρ, ΠΌΠ΅Π΄ΠΈΠ°Π½Π° Π²ΠΎΠ·ΡΠ°ΡΡΠ° Π΄Π΅Π±ΡΡΠ° ΠΠ¨ - 33 Π³ΠΎΠ΄Π°. ΠΡΡΠ΅ΠΊΡΠΈΠ²Π½ΠΎΡΡΡ Π΄Π»ΠΈΡΠ΅Π»ΡΠ½ΠΎΠΉ ΡΠ΅ΡΠ°ΠΏΠΈΠΈ ΠΌΠ°Π»ΡΠΌΠΈ Π΄ΠΎΠ·Π°ΠΌΠΈ ΠΠΡ 151 Π±ΠΎΠ»ΡΠ½ΠΎΠ³ΠΎ (ΠΌΠ΅Π΄ΠΈΠ°Π½Π° - 10 Π»Π΅Ρ), ΠΌΠ°Π»ΡΠΌΠΈ Π΄ΠΎΠ·Π°ΠΌΠΈ ΠΠ¦Ρ 147 Π±ΠΎΠ»ΡΠ½ΡΡ
(ΠΌΠ΅Π΄ΠΈΠ°Π½Π° - 5 Π»Π΅Ρ) ΠΎΡΠ΅Π½Π΅Π½Π° Π² ΡΡΠ°Π²Π½Π΅Π½ΠΈΠΈ Ρ ΡΠ΅Π·ΡΠ»ΡΡΠ°ΡΠ°ΠΌΠΈ Π»Π΅ΡΠ΅Π½ΠΈΡ 94 Π±ΠΎΠ»ΡΠ½ΡΡ
, ΠΏΠΎΠ»ΡΡΠ°Π²ΡΠΈΡ
ΡΠΎΠ»ΡΠΊΠΎ Β«ΠΎΡΡΠ°Π»ΡΠΌΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΠ΅Β» ΠΈ Β«ΡΡΠΎΠΌΠ°ΡΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΠ΅Β» ΠΌΠ΅ΡΠΎΠ΄Ρ ΡΠ΅ΡΠ°ΠΏΠΈΠΈ. Π Π΅Π·ΡΠ»ΡΡΠ°ΡΡ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ. ΠΠΎΠΊΠ°Π·Π°Π½ΠΎ, ΡΡΠΎ ΡΠ΅ΡΠ°ΠΏΠΈΡ ΠΌΠ°Π»ΡΠΌΠΈ Π΄ΠΎΠ·Π°ΠΌΠΈ ΠΠ ΠΈ ΠΠ¦ ΠΏΠΎΠ»ΠΎΠΆΠΈΡΠ΅Π»ΡΠ½ΠΎ Π²Π»ΠΈΡΠ»Π° Π½Π° Π½Π΅ΠΊΠΎΡΠΎΡΡΠ΅ ΠΆΠ΅Π»Π΅Π·ΠΈΡΡΡΠ΅ ΠΈ Π²Π½Π΅ΠΆΠ΅Π»Π΅Π·ΠΈΡΡΡΠ΅ ΠΏΡΠΎΡΠ²Π»Π΅Π½ΠΈΡ Π·Π°Π±ΠΎΠ»Π΅Π²Π°Π½ΠΈΡ, ΠΎΠ΄Π½Π°ΠΊΠΎ ΠΊ ΡΠ½ΠΈΠΆΠ΅Π½ΠΈΡ Π²Π΅ΡΠΎΡΡΠ½ΠΎΡΡΠΈ ΡΠ°Π·Π²ΠΈΡΠΈΡ Π»ΠΈΠΌΡΠΎΠΏΡΠΎΠ»ΠΈΡΠ΅ΡΠ°ΡΠΈΠ²Π½ΡΡ
Π·Π°Π±ΠΎΠ»Π΅Π²Π°Π½ΠΈΠΉ (Ρ=0,036) ΠΈ ΠΏΠΎΠ²ΡΡΠ΅Π½ΠΈΡ Π²ΡΠΆΠΈΠ²Π°Π΅ΠΌΠΎΡΡΠΈ Π±ΠΎΠ»ΡΠ½ΡΡ
Ρ ΠΠ¨ ΠΏΡΠΈΠ²ΠΎΠ΄ΠΈΠ»Π° ΡΠΎΠ»ΡΠΊΠΎ ΡΠ΅ΡΠ°ΠΏΠΈΡ ΠΠ¦ (Ρ=0,037). Π Π°Π·ΡΠ°Π±ΠΎΡΠ°Π½Ρ ΠΈ ΠΏΡΠ΅Π΄ΡΡΠ°Π²Π»Π΅Π½Ρ ΠΌΠ΅ΡΠΎΠ΄ΠΈΠΊΠΈ Π»ΠΎΠΊΠ°Π»ΡΠ½ΠΎΠ³ΠΎ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΡ ΠΠ ΠΏΡΠΈ ΡΡΠΆΠ΅Π»ΡΡ
ΠΎΡΡΠ°Π»ΡΠΌΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΡ
ΠΈ ΡΡΠΎΠΌΠ°ΡΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΡ
ΠΏΡΠΎΡΠ²Π»Π΅Π½ΠΈΡΡ
ΠΠ¨. Π£ 150 Π±ΠΎΠ»ΡΠ½ΡΡ
Ρ ΠΠ¨ Ρ ΡΡΠΆΠ΅Π»ΡΠΌΠΈ ΡΠΈΡΡΠ΅ΠΌΠ½ΡΠΌΠΈ ΠΏΡΠΎΡΠ²Π»Π΅Π½ΠΈΡΠΌΠΈ ΠΏΡΠ΅Π΄ΡΡΠ°Π²Π»Π΅Π½Ρ ΠΏΠΎΠΊΠ°Π·Π°Π½ΠΈΡ ΠΈ ΠΏΡΠΎΡΠΈΠ²ΠΎΠΏΠΎΠΊΠ°Π·Π°Π½ΠΈΡ Π΄Π»Ρ ΠΏΡΠΎΠ²Π΅Π΄Π΅Π½ΠΈΡ ΠΏΡΠ»ΡΡ-ΡΠ΅ΡΠ°ΠΏΠΈΠΈ (ΠΠ’), ΠΊΠΎΠΌΠ±ΠΈΠ½ΠΈΡΠΎΠ²Π°Π½Π½ΠΎΠΉ ΠΠ’, ΠΏΠ»Π°Π·ΠΌΠ°ΡΠ΅ΡΠ΅Π·Π°, ΠΊΡΠΈΠΎΠ°ΡΠ΅ΡΠ΅Π·Π° ΠΈ Π΄Π²ΠΎΠΉΠ½ΠΎΠΉ ΡΠΈΠ»ΡΡΡΠ°ΡΠΈΠΈ ΠΏΠ»Π°Π·ΠΌΡ Π² ΡΠΎΡΠ΅ΡΠ°Π½ΠΈΠΈ Ρ ΠΊΠΎΠΌΠ±ΠΈΠ½ΠΈΡΠΎΠ²Π°Π½Π½ΠΎΠΉ ΠΠ’. ΠΠΎΠΊΠ°Π·Π°Π½ΠΎ, ΡΡΠΎ Π½Π°Π·Π½Π°ΡΠ΅Π½ΠΈΠ΅ ΠΌΠ°Π»ΡΡ
Π΄ΠΎΠ· ΠΠ ΠΈ ΠΠ’ Ρ ΠΆΠ΅Π½ΡΠΈΠ½ Ρ ΠΠ¨ Ρ ΡΠΎΡ
ΡΠ°Π½Π΅Π½Π½ΡΠΌ ΠΌΠ΅Π½ΡΡΡΡΠ°Π»ΡΠ½ΡΠΌ ΡΠΈΠΊΠ»ΠΎΠΌ ΠΏΡΠΈΠ²ΠΎΠ΄ΠΈΡ ΠΊ Π±ΠΎΠ»Π΅Π΅ ΡΠ°Π½Π½Π΅ΠΌΡ Π½Π°ΡΡΡΠΏΠ»Π΅Π½ΠΈΡ ΠΌΠ΅Π½ΠΎΠΏΠ°ΡΠ·Ρ ΠΈ ΡΠΏΠΎΡΠΎΠ±ΡΡΠ²ΡΠ΅Ρ ΡΠ°Π·Π²ΠΈΡΠΈΡ Π³Π»ΡΠΊΠΎΠΊΠΎΡΡΠΈΠΊΠΎΠΈΠ΄Π½ΠΎΠ³ΠΎ ΠΈ ΠΏΠΎΡΡΠΌΠ΅Π½ΠΎΠΏΠ°ΡΠ·Π°Π»ΡΠ½ΠΎΠ³ΠΎ ΠΎΡΡΠ΅ΠΎΠΏΠΎΡΠΎΠ·Π° Π² ΠΎΡΠ΄Π°Π»Π΅Π½Π½ΡΠ΅ ΡΡΠΎΠΊΠΈ. ΠΡΠ΅Π½Π΅Π½Ρ Π½Π°ΠΈΠ±ΠΎΠ»Π΅Π΅ Π·Π½Π°ΡΠΈΠΌΡΠ΅ ΠΏΠΎΠ±ΠΎΡΠ½ΡΠ΅ ΠΏΡΠΎΡΠ²Π»Π΅Π½ΠΈΡ ΡΠ΅ΡΠ°ΠΏΠΈΠΈ ΠΠ ΠΈ ΠΠ¦ ΠΏΡΠΈ ΡΠ°Π·Π»ΠΈΡΠ½ΡΡ
Π²Π°ΡΠΈΠ°Π½ΡΠ°Ρ
ΡΠ΅ΡΠ°ΠΏΠΈΠΈ ΠΠ¨
ΠΠ½Π°Π»ΠΈΠ· ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ ΡΠΈΡΡΠΎΠ²ΠΎΠΉ ΡΠ΅Π°Π»ΠΈΠ·Π°ΡΠΈΠΈ ΠΈΠ·ΠΌΠ΅ΡΠ΅Π½ΠΈΡ ΠΏΠ°ΡΠ°ΠΌΠ΅ΡΡΠΎΠ² ΡΠΈΠ³Π½Π°Π»ΠΎΠ²
The article reviews an algorithm of digital signal processing to implement computation of the amplitude of signal envelope, its frequency and initial phase. It also estimates performance accuracy reached owing to ideal model algorithm and its implementation. The practical implementation of the algorithm is part of the system exploring signal parameters.The goal was to test the algorithm capability to work in conditions of a priori uncertainty, having only input samples and measuring radio signal Gate [1], its capability to calculate the parameters of the radio signal with different modulation types while maintaining accuracy. Characterisation of radio signals is a very important process in radio engineering as having radio signal parameters can provide information about modulation radio signal and, consequently, information contained therein.Due to the high complexity of the algorithm, its mathematical description seems to be impossible, so construction of simulation models was chosen as a research tool. Practical implementation of the algorithm comprises a Hilbert transformer based on the FIR filter, a calculating unit of the amplitude envelope, a calculating unit of the phase detector signal, and a detector of the phase jumps. The article has discussed the algorithm in-detail and has shown deviations of values obtained in the practical implementation of the algorithm against the modeling ones. For the development and debugging of the algorithm programme, was created the Mathcad environment. The simulation results are also presented in the article.The running algorithm was tested under pulsed signal at the input, pulse signal at the FMmodulated input, and FM modulation noisy signal. Its accuracy is evaluated in comparison with the model. Dependence of envelope amplitude RMS versus dimensionality of the input signal was investigated as well. As a result, the optimum dimension of input signal was selected, as well as the accuracy of the algorithm in terms of a priori uncertainties was estimated.Π ΡΡΠ°ΡΡΠ΅ ΡΠ°ΡΡΠΌΠΎΡΡΠ΅Π½ Π°Π»Π³ΠΎΡΠΈΡΠΌ ΡΠΈΡΡΠΎΠ²ΠΎΠΉ ΠΎΠ±ΡΠ°Π±ΠΎΡΠΊΠΈ ΡΠΈΠ³Π½Π°Π»ΠΎΠ², ΡΠ΅Π°Π»ΠΈΠ·ΡΡΡΠΈΠΉ Π²ΡΡΠΈΡΠ»Π΅Π½ΠΈΠ΅ Π°ΠΌΠΏΠ»ΠΈΡΡΠ΄Ρ ΠΎΠ³ΠΈΠ±Π°ΡΡΠ΅ΠΉ ΡΠΈΠ³Π½Π°Π»Π°, Π΅Π³ΠΎ ΡΠ°ΡΡΠΎΡΡ ΠΈ Π½Π°ΡΠ°Π»ΡΠ½ΠΎΠΉ ΡΠ°Π·Ρ, Π° ΡΠ°ΠΊ ΠΆΠ΅ Π±ΡΠ»Π° ΠΏΡΠΎΠ²Π΅Π΄Π΅Π½Π° ΠΎΡΠ΅Π½ΠΊΠ° ΡΠΎΡΠ½ΠΎΡΡΠΈ Π²ΡΡΠΈΡΠ»Π΅Π½Π½ΡΡ
Ρ
Π°ΡΠ°ΠΊΡΠ΅ΡΠΈΡΡΠΈΠΊ. ΠΡΠ°ΠΊΡΠΈΡΠ΅ΡΠΊΠ°Ρ ΡΠ΅Π°Π»ΠΈΠ·Π°ΡΠΈΡ Π°Π»Π³ΠΎΡΠΈΡΠΌΠ° ΡΠ²Π»ΡΠ΅ΡΡΡ ΡΠ°ΡΡΡΡ ΡΠΈΡΡΠ΅ΠΌΡ, ΠΈΡΡΠ»Π΅Π΄ΡΡΡΠ΅ΠΉ ΠΏΠ°ΡΠ°ΠΌΠ΅ΡΡΡ ΡΠΈΠ³Π½Π°Π»Π°. Π¦Π΅Π»ΡΡ ΡΠ°Π±ΠΎΡΡ Π±ΡΠ»ΠΎ ΠΏΡΠΎΠ²Π΅ΡΠΈΡΡ ΡΠΏΠΎΡΠΎΠ±Π½ΠΎΡΡΡ Π°Π»Π³ΠΎΡΠΈΡΠΌΠ° ΡΠ°Π±ΠΎΡΠ°ΡΡ Π² ΡΡΠ»ΠΎΠ²ΠΈΡΡ
Π°ΠΏΡΠΈΠΎΡΠ½ΠΎΠΉ Π½Π΅ΠΎΠΏΡΠ΅Π΄Π΅Π»Π΅Π½Π½ΠΎΡΡΠΈ, ΠΈΠΌΠ΅Ρ Π½Π° Π²Ρ
ΠΎΠ΄Π΅ ΡΠΎΠ»ΡΠΊΠΎ ΠΎΡΡΡΠ΅ΡΡ ΡΠ°Π΄ΠΈΠΎΡΠΈΠ³Π½Π°Π»Π° ΠΈ ΠΈΠ·ΠΌΠ΅ΡΠΈΡΠ΅Π»ΡΠ½ΠΎΠ³ΠΎ ΡΡΡΠΎΠ±Π° [1]. ΠΠ²ΠΈΠ΄Ρ Π²ΡΡΠΎΠΊΠΎΠΉ ΡΠ»ΠΎΠΆΠ½ΠΎΡΡΠΈ Π°Π»Π³ΠΎΡΠΈΡΠΌΠ°, ΠΎΠΏΠΈΡΠ°Π½ΠΈΠ΅ Π΅Π³ΠΎ ΠΌΠ°ΡΠ΅ΠΌΠ°ΡΠΈΡΠ΅ΡΠΊΠΈ Π½Π΅ ΠΏΡΠ΅Π΄ΡΡΠ°Π²Π»ΡΠ΅ΡΡΡ Π²ΠΎΠ·ΠΌΠΎΠΆΠ½ΡΠΌ, ΠΏΠΎΡΡΠΎΠΌΡ Π² ΠΊΠ°ΡΠ΅ΡΡΠ²Π΅ Π°ΠΏΠΏΠ°ΡΠ°ΡΠ° Π΄Π»Ρ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ Π±ΡΠ»ΠΎ Π²ΡΠ±ΡΠ°Π½ΠΎ ΠΏΠΎΡΡΡΠΎΠ΅Π½ΠΈΠ΅ ΠΈΠΌΠΈΡΠ°ΡΠΈΠΎΠ½Π½ΠΎΠΉ ΠΌΠΎΠ΄Π΅Π»ΠΈ. Π ΡΠ΅Π·ΡΠ»ΡΡΠ°ΡΠ΅ ΡΠ°Π±ΠΎΡΡ Π±ΡΠ»Π° ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½Π° Π·Π°Π²ΠΈΡΠΈΠΌΠΎΡΡΡ Π‘ΠΠ Π°ΠΌΠΏΠ»ΠΈΡΡΠ΄Ρ ΠΎΠ³ΠΈΠ±Π°ΡΡΠ΅ΠΉ Π² Π·Π°Π²ΠΈΡΠΈΠΌΠΎΡΡΠΈ ΠΎΡ ΡΠ°Π·ΠΌΠ΅ΡΠ½ΠΎΡΡΠΈ Π²Ρ
ΠΎΠ΄Π½ΠΎΠ³ΠΎ ΡΠΈΠ³Π½Π°Π»Π°, Π° ΡΠ°ΠΊ ΠΆΠ΅ ΠΎΡΠ΅Π½Π΅Π½Π° ΡΠΎΡΠ½ΠΎΡΡΡ ΡΠ°Π±ΠΎΡΡ Π°Π»Π³ΠΎΡΠΈΡΠΌΠ° Π² ΡΡΠ»ΠΎΠ²ΠΈΡΡ
Π°ΠΏΡΠΈΠΎΡΠ½ΠΎΠΉ Π½Π΅ΠΎΠΏΡΠ΅Π΄Π΅Π»Π΅Π½Π½ΠΎΡΡΠΈ. DOI: 10.7463/rdopt.0515.081296
Using CAST-test to investigate human specific hypersensitivity to the anthrax pathogen
We present the results of applying functional cytometric test of antigen-stimulated activation basophils to assess specific immunological reactivity in the people with anthrax, and immunized with anthrax vaccine. As a criterion for antigen-specific basophil activation, we measured expression of the CD63 membrane receptor, which reflects the process of anaphylactic basophil degranulation. To determine spontaneous and antigen-induced activation of basophils (CCR3+CD63+), a FlowCAST reagent kit (Buhlmann laboratories AG, Switzerland) was used. Anthraxin, an experimental anthrax allergen (a hydrolysate the Bacillus anthracis STI-1 strain), manufactured by the Stavropol Anti-Plague Institute, was used as a specific antigen. As based on clinical and experimental data, a threshold value of > 10% of anthraxin-activated (CCR3+CD63+) basophils was accepted for the in vitro immunodiagnostic CAST test, as a laboratory criterion for the subjects exhibiting specific immune response, i.e., IgE-mediated sensitization. It was shown that, in anthrax patients within one week after onset of the disease (3-7 days), a positive CAST result was obtained in 92.3% cases; the levels of specific basophil activation with anthraxin averaged 37.9% (12.01 Γ· 78.9%). Immunological examination of individuals three weeks (21 days) after vaccination against anthrax revealed CAST-positivity in all the vaccinated persons. Intensity of anthraxin-induced basophil activation the vaccinated subjects was ranged from 10.87 to 30.03%, averaging 17.86%. The overall values of spontaneous and specific activation ranged within 12.39 Γ· 41.46%. The study opens prospectives for implementation of basophil antigenic activation test in the Flow CAST format in diagnostics of anthrax and to identify specific immune rearrangements after vaccination in humans, as an index of actual vaccination rates. Usage of CAST test with anthraxin makes it possible to identify anthrax patients at the early stages (2-4 days after onset of the disease) including, among patients with an increased CCR3+CD63+ background values, evaluation of immunological efficiency in the cohorts at risk for vaccination. At the same time, it was found that a significant decrease in diagnostic sensitivity of CAST test could be observed in the patients immune to anthrax pathogen who received intensive antibacterial and pathogenetic therapy at the early stages of infection, including glucocorticosteroids (anti-inflammatory drugs) and desensitizing agents that inhibit the degree of hypersensitivity development and its expression
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