18 research outputs found
Antiviral Activity of Extracts from Basidiomycetes for Orthopoxviruses
Carried out was evaluation of the effect of water extracts from basidiomycetes in Vero cell culture on variola virus, and vaccinia virus. Antiviral effect demonstrated
Inonotus obliquus (Chaga), befungin and melanin from chaga,
Ganoderma applanatum (Artistβs Bracket)
, Fomitopsis officinalis (Larch Fungus)
Studies of Anti-Viral Activity of Chemically Synthesized Compounds against Orthopoxviruses <I>in vitro</I>
for ST-246 and NIOC-14 is within the range of 0,001-0,004 Β΅g/ml, and IS for both of them is > 100000. In addition, ST-246 and NIOC-14 chemical compound efficacy, concentrated up to 0,0125; 0,025 and 0,05 Β΅g/ml, in accordance with prophylactic charts describing an impact on ectromelia virus (EV) infectivity
in vitro, is consequently 0,6; 3 and 1 lg higher than in case of compound application after an hour of Vero cells infection with EV
2-ΡΠΈΠΊΠ»ΠΎΠ°Π»ΠΊΠΈΠ»ΠΈΠΌΠΈΠ½ΠΎ-5-(4-Π½ΠΈΡΡΠΎΡΠ΅Π½ΠΈΠ»)-1,3,4-ΡΠΈΠ°Π΄ΠΈΠ°Π·ΠΈΠ½Ρ, ΠΎΠ±Π»Π°Π΄Π°ΡΡΠΈΠ΅ Π±ΠΈΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΎΠΉ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΡΡ ΠΏΡΠΎΡΠΈΠ² Π²ΠΈΡΡΡΠΎΠ² ΠΎΡΠΏΡ
ΠΠ·ΠΎΠ±ΡΠ΅ΡΠ΅Π½ΠΈΠ΅ ΠΎΡΠ½ΠΎΡΠΈΡΡΡ ΠΊ Π½ΠΎΠ²ΡΠΌ 2-ΡΠΈΠΊΠ»ΠΎΠ°Π»ΠΊΠΈΠ»ΠΈΠΌΠΈΠ½ΠΎ-5-(4-Π½ΠΈΡΡΠΎΡΠ΅Π½ΠΈΠ»)-1,3,4-ΡΠΈΠ°Π΄ΠΈΠ°Π·ΠΈΠ½Π°ΠΌ ΠΎΠ±ΡΠ΅ΠΉ ΡΠΎΡΠΌΡΠ»Ρ I: Π³Π΄Π΅ ΠΏΠΈΠΏΠ΅ΡΠΈΠ΄ΠΈΠ½ΠΎ; ΠΏΠΈΡΡΠΎΠ»ΠΈΠ΄ΠΈΠ½ΠΎ; ΠΌΠ΅ΡΠΈΠ»ΠΏΠΈΠΏΠ΅ΡΠ°Π·ΠΈΠ½ΠΎ; Π³Π΅ΠΊΡΠ°ΠΌΠ΅ΡΠΈΠ»Π΅Π½ΠΈΠΌΠΈΠ½ΠΎ, ΠΊΠΎΡΠΎΡΡΠ΅ ΠΎΠ±Π»Π°Π΄Π°ΡΡ Π±ΠΈΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΎΠΉ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΡΡ ΠΏΡΠΎΡΠΈΠ² Π²ΠΈΡΡΡΠΎΠ² ΠΎΡΠΏΡ. Π’Π΅Ρ
Π½ΠΈΡΠ΅ΡΠΊΠΈΠΉ ΡΠ΅Π·ΡΠ»ΡΡΠ°Ρ - ΠΏΠΎΠ»ΡΡΠ΅Π½Ρ Π½ΠΎΠ²ΡΠ΅ Π±ΠΈΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈ Π°ΠΊΡΠΈΠ²Π½ΡΠ΅ ΡΠΎΠ΅Π΄ΠΈΠ½Π΅Π½ΠΈΡ, ΠΎΠ±Π»Π°Π΄Π°ΡΡΠΈΠ΅ ΠΏΡΠΎΡΠΈΠ²ΠΎΠ²ΠΈΡΡΡΠ½ΡΠΌ Π΄Π΅ΠΉΡΡΠ²ΠΈΠ΅ΠΌ, Π² ΡΠ°ΡΡΠ½ΠΎΡΡΠΈ Π² ΠΎΡΠ½ΠΎΡΠ΅Π½ΠΈΠΈ Π²ΠΈΡΡΡΠΎΠ² ΠΎΡΠΏΡ. 1 Π½.ΠΏ. Ρ-Π»Ρ, 1 ΡΠ°Π±Π». ΠΠ·ΠΎΠ±ΡΠ΅ΡΠ΅Π½ΠΈΠ΅ ΠΎΡΠ½ΠΎΡΠΈΡΡΡ ΠΊ ΠΎΠ±Π»Π°ΡΡΠΈ Π±ΠΈΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈ Π°ΠΊΡΠΈΠ²Π½ΡΡ
ΡΠΎΠ΅Π΄ΠΈΠ½Π΅Π½ΠΈΠΉ, ΠΊΠ°ΡΠ°Π΅ΡΡΡ ΡΠ°Π·ΡΠ°Π±ΠΎΡΠΊΠΈ Π½ΠΎΠ²ΡΡ
ΠΏΡΠΎΠΈΠ·Π²ΠΎΠ΄Π½ΡΡ
1,3,4-ΡΠΈΠ°Π΄ΠΈΠ°Π·ΠΈΠ½ΠΎΠ², ΠΈ ΠΏΡΠ΅Π΄Π½Π°Π·Π½Π°ΡΠ΅Π½ΠΎ Π΄Π»Ρ Π»Π΅ΡΠ΅Π½ΠΈΡ ΠΈ ΠΏΡΠΎΡΠΈΠ»Π°ΠΊΡΠΈΠΊΠΈ Π·Π°Π±ΠΎΠ»Π΅Π²Π°Π½ΠΈΠΉ, Π²ΡΠ·ΡΠ²Π°Π΅ΠΌΡΡ
ΠΏΠ°ΡΠΎΠ³Π΅Π½Π½ΡΠΌΠΈ Π΄Π»Ρ ΡΠ΅Π»ΠΎΠ²Π΅ΠΊΠ° ΠΈ ΠΆΠΈΠ²ΠΎΡΠ½ΡΡ
Π²ΠΈΡΡΡΠ°ΠΌΠΈ, Π° ΡΠ°ΠΊΠΆΠ΅ Π²ΠΈΡΡΡΠΎΠΌ ΠΎΡΠΏΠ°Π²Π°ΠΊΡΠΈΠ½Ρ (Π»Π΅ΡΠ΅Π½ΠΈΠ΅ ΠΏΠΎΡΡΠ²Π°ΠΊΡΠΈΠ½Π°Π»ΡΠ½ΡΡ
ΠΎΡΠ»ΠΎΠΆΠ½Π΅Π½ΠΈΠΉ)
Synthesis and Antiviral Properties of Camphor-Derived Iminothiazolidine-4-Ones and 2,3-Dihydrothiazoles
A set of heterocyclic products was synthesized from natural (+)-camphor and semi-synthetic (β)-camphor. Then, 2-Imino-4-thiazolidinones and 2,3-dihydrothiazoles were obtained using a three-step procedure. For the synthesized compounds, their antiviral activity against the vaccinia virus and Marburg virus was studied. New promising agents active against both viruses were found among the tested compounds
Aggregation of Multimodal ICE-MS Data into Joint Classifier Increases Quality of Brain Cancer Tissue Classification
Mass spectrometry fingerprinting combined with multidimensional data analysis has been proposed in surgery to determine if a biopsy sample is a tumor. In the specific case of brain tumors, it is complicated to obtain control samples, leading to model overfitting due to unbalanced sample cohorts. Usually, classifiers are trained using a single measurement regime, most notably single ion polarity, but mass range and spectral resolution could also be varied. It is known that lipid groups differ significantly in their ability to produce positive or negative ions; hence, using only one polarity significantly restricts the chemical space available for sample discrimination purposes. In this work, we have developed an approach employing mass spectrometry data obtained by eight different regimes of measurement simultaneously. Regime-specific classifiers are trained, then a mixture of experts techniques based on voting or mean probability is used to aggregate predictions of all trained classifiers and assign a class to the whole sample. The aggregated classifiers have shown a much better performance than any of the single-regime classifiers and help significantly reduce the effect of an unbalanced dataset without any augmentation
Shapley Value as a Quality Control for Mass Spectra of Human Glioblastoma Tissues
The automatic processing of high-dimensional mass spectrometry data is required for the clinical implementation of ambient ionization molecular profiling methods. However, complex algorithms required for the analysis of peak-rich spectra are sensitive to the quality of the input data. Therefore, an objective and quantitative indicator, insensitive to the conditions of the experiment, is currently in high demand for the automated treatment of mass spectrometric data. In this work, we demonstrate the utility of the Shapley value as an indicator of the quality of the individual mass spectrum in the classification task for human brain tumor tissue discrimination. The Shapley values are calculated on the training set of glioblastoma and nontumor pathological tissues spectra and used as feedback to create a random forest regression model to estimate the contributions for all spectra of each specimen. As a result, it is shown that the implementation of Shapley values significantly accelerates the data analysis of negative mode mass spectrometry data alongside simultaneous improving the regression modelsβ accuracy
Spherical Sampler Probes Enhance the Robustness of Ambient Ionization Mass Spectrometry for Rapid Drugs Screening
Ambient ionization mass spectrometry has become one of the most promising approaches for rapid and high-throughput screening of small molecules in complex biological matrices for emergency medicine, forensics, and food and agriculture applications. The simple procedures for sample collection and ionization without additional pretreatment are vital in these fields. Many efforts have been devoted to modifying various ambient ionization techniques to simplify the procedures and improve the robustness and sensitivity of the methods. Here, we demonstrate the implementation of rigid spherical sampler probes to improve the robustness of touch spray ionization mass spectrometry. The sphericity of the probes increases the stability of the cone-jet mode of electrospray, reduces the requirements for fine positioning of a sampler in the ion source, and decreases the possibility of corona discharge occurrence. The utilization of spherical sampler probes allows fast, non-invasive sampling, followed by rapid analysis for various drugs of different chemical classes in complex biological matrices, such as the whole blood or sebum collected from the skin surface. The linearity of the analytical signal response from drug concentration confirms the possibility of creating a simple semiquantitative method for small molecules monitoring using spherical sampler probes
Properties of Pandemic Influenza Virus Strains Isolated in the Territory of Russia
The first cases of the disease caused by pandemic (H1N1) 2009 influenza virus in the territory of the Russian Federation were registered at the end of May, 2009. 3 strains of pandemic (H1N1)2009 influenza virus were isolated from patients. Properties of the strains isolated in the territory of Russia were studied in comparison with those of two reference strains A/California/04/2009(H1N1) and A/California/07/2009(H1N1)v. Analysis of primary gene sequence and examination of biological properties of the strains isolated in the territory of Russia suggested their close relationship with A/California/04/2009(H1N1)v and A/California/07/2009(H1N1)v strains