43 aphid species, living on 39 host plants, were recorded from the Ajameti reserve (Georgia) in 2008. Two species (Aphis rubiae Narzikulov, 1964 and A. violae Schouteden, 1900) were recorded for the first time from Transcaucasia, while fourteen species were new records for Imereti Region. Fundatrices of Macrosiphum symphyti Barjadze et Chakvetadze, 2008 were found for the first time. Tritrophic relationships between aphids, their natural enemies and host plants were studie