Determination of bacterial antigens using a multichannel immunoenzyme amperometric sensor

A procedure for the individual determination of the antigens of the conditionally pathogenic micro-organisms Streptococcus pyogenes and Staphylococcus aureus present in combination using a multichannel immunoenzyme amperometric sensor based on a screen-printed electrode was developed. Conditions for fabricating the biosensing part and for the operation of the sensor (the matrix component, the ratio between biocomponents, and the substrate concentration) were chosen. The determination limits of bacterial antigens obtained using the analytical device were equal to 5 × 10-9 mg/mL. It was found that bacterial antigens simultaneously present in serums from patients can be determined using the sensor developed. © Pleiades Publishing, Inc., 2006

Levosalbutamol as alternative to drugs on the basis of racemic salbutamol: Review of the results of pre-clinical research

The aim of the work is to conduct an analytical review of the results of preclinical studies of levosalbutamol. The review discusses the pharmacodynamic features of the R-stereoisomer of salbutamol in vitro. The chemical bases of interaction of levosalbutamol with β2-adrenoreceptors, intracellular signaling cascades associated with β2-adrenoreceptors, and structural features of clinically significant ligands of β2-adrenoreceptors are presented. Broncholytic activity, influence on the contractility of the diaphragmatic muscles, mucociliary clearance of R-salbutamol in comparison with racemic salbutamol are described. The data presented indicate that all known β2-adrenergic receptor-dependent effects of racemic salbutamol, including bronchodilation, are realized by its R-enantiomer. There is evidence that the regular inhalation administration of racemic salbutamol is accompanied by a partial decrease in the bronchoprotective effect and an increase in airway hyperreactivity in response to the action of provocative factors. It was found that the development of hyperreactivity of the respiratory tract is excluded in the case of regular inhalation of levosalbutamol. Possible mechanisms of the paradoxical bronchoconstrictor effect of the salbutamol dystomer are described. This article shows the beneficial effect of levosalbutamol on mucociliary clearance, its anti-inflammatory activity and antiallergic effect. The image data are compared between the enantiomers and the racemate of salbutamol. Special attention is paid to the pharmacokinetics of enantiomers of salbutamol. The data presented from the preclinical studies provide evidence of chiral inversion of stereoisomers of salbutamol

Determination of bacterial antigens using a multichannel immunoenzyme amperometric sensor

A procedure for the individual determination of the antigens of the conditionally pathogenic micro-organisms Streptococcus pyogenes and Staphylococcus aureus present in combination using a multichannel immunoenzyme amperometric sensor based on a screen-printed electrode was developed. Conditions for fabricating the biosensing part and for the operation of the sensor (the matrix component, the ratio between biocomponents, and the substrate concentration) were chosen. The determination limits of bacterial antigens obtained using the analytical device were equal to 5 × 10-9 mg/mL. It was found that bacterial antigens simultaneously present in serums from patients can be determined using the sensor developed. © Pleiades Publishing, Inc., 2006

Determination of bacterial antigens using a multichannel immunoenzyme amperometric sensor

A procedure for the individual determination of the antigens of the conditionally pathogenic micro-organisms Streptococcus pyogenes and Staphylococcus aureus present in combination using a multichannel immunoenzyme amperometric sensor based on a screen-printed electrode was developed. Conditions for fabricating the biosensing part and for the operation of the sensor (the matrix component, the ratio between biocomponents, and the substrate concentration) were chosen. The determination limits of bacterial antigens obtained using the analytical device were equal to 5 × 10-9 mg/mL. It was found that bacterial antigens simultaneously present in serums from patients can be determined using the sensor developed. © Pleiades Publishing, Inc., 2006

Determination of bacterial antigens using a multichannel immunoenzyme amperometric sensor

A procedure for the individual determination of the antigens of the conditionally pathogenic micro-organisms Streptococcus pyogenes and Staphylococcus aureus present in combination using a multichannel immunoenzyme amperometric sensor based on a screen-printed electrode was developed. Conditions for fabricating the biosensing part and for the operation of the sensor (the matrix component, the ratio between biocomponents, and the substrate concentration) were chosen. The determination limits of bacterial antigens obtained using the analytical device were equal to 5 × 10-9 mg/mL. It was found that bacterial antigens simultaneously present in serums from patients can be determined using the sensor developed. © Pleiades Publishing, Inc., 2006

New educational technologies as a means of cross - regional cooperation in the field of food raw material and product safety control

International e-learning resource in the field of food product quality and veterinary disease control based on Government requirements of the European Union and Eurasian Economic Union has been created and tested. This resource consists of the one general professional cycle and five professional modules, about 60% of them are in distance e-learning format. The modularity of the educational resource allows the employer or students to choose a training program format, as well as the amount of distance learning or classroom training hours for different groups of specialists in the field of food quality and veterinary control: chiefs of quality control center or laboratories, veterinarians, microbiologists, specialists of veterinary diagnostics laboratories, chemical engineers. The involvement of participants from EU and EEU-countries in the educational process is important for the development of interregional and international activities, the creation of the unified legal framework in the field of food security in many countries