Application of the wax moth (Galeria melonella) larvae extract for correction of the immune status of athletes during the recovery period

Objective: to study dynamics of parameters of system immunity of athletes during the recovery period against the background of the combined reception of antler bathtubs and extract of larvae of a wax moth. Materials and methods: dynamics of parameters of the immune system of 28 athletes (skiers) had been studied during the recovery period. The main group of athletes (14 people) received antler bathtubs in combination with intake of extract of larvae of a wax moth, and group of comparison - only antler bathtubs. Results: the analysis of the results of the survey showed that the combined reception of the extract of the larvae of the wax moth and antler baths by athletes has a pronounced effect on the dynamics of the studied parameters. The significant augmentation of initially lowered subpopulations of lymphocytes became perceptible (CD3+, CD4+, CD8+). There was an essential depression of concentration of pro-inflammatory cytokines of TNF-a and IL-6 in blood serum and depression of spontaneous production of pro-inflammatory cytokines of TNF-a (p =0,010), IL-6 and IL lß by blood cells. Conclusions: the obtained data confirm the expressed immunomodulatory effect of extract of larvae of a wax moth in athletes during the recovery period

ПОВЕДЕНЧЕСКАЯ АКТИВНОСТЬ КРЫС В «ОТКРЫТОМ ПОЛЕ» ПОСЛЕ СВЕТОВОЙ ИЛИ ТЕМНОВОЙ ДЕПРИВАЦИЙ И ФИЗИЧЕСКОГО ПЕРЕУТОМЛЕНИЯ

Aim. As is known, various stressful loads and their combination lead to unequal direction and degree of psychological and emotional instability. In this regard, one of the pressing issues becomes regulation and correction of psycho-emotional conditions of the person in the complicated conditions of activity, such as athletes during training and competition. To develop appropriate stress-corrective programs should their experimental validation. Therefore, the aim of this study was to investigate the behavioral activity of rats in the “open field” after dark or light deprivation and physical fatigue.Materials and methods. The experimental study was performed on the 40 adult male rats “Wistar”. The experimental groups for 10 days were kept in an artificial bright light (150 lx) or darkness (2–3 lx) for the induction of desynchronozes. Method of forced swimming until complete exhaustion was chosen for the model of physical fatigue. The animals in all groups evaluated behavioral activity in the “open field” in daylight conditions after 24 h after swim test.Results. It was established that in the control group in the terms of natural lighting day after 5 days of daily physical activity occurred depression of the active-search behavior in the “open field”. It was expressed in reducing the number of crossed squares and vertical struts in comparison to intact animals receiving no load. In the groups of animals, which kept in a dark or light deprivation until the presentation of the swim test there was an increase in passive-defensive behavior in the “open field”, which was reflected in an increase in acts of grooming and defecation. Цель. Как известно, разные стрессирующие нагрузки и их сочетание ведут к неодинаковой направленности и выраженности психоэмоциональной нестабильности. В связи с этим одним из актуальных вопросов становится регуляция и коррекция психоэмоциональных состояний человека в сложных условиях деятельности, например спортсменов в период тренировок и соревнований. Для разработки адекватных стресс-корректирующих программ необходимо их экспериментальное обоснование. Поэтому целью настоящего исследования явилось изучение поведенческой активности крыс в «открытом поле» после световой или темновой деприваций и физического переутомления.Материал и методы. Экспериментальное исследование выполнено на 40 половозрелых крысах-самцах породы Wistar. Для индукции экспериментального десинхроноза животные опытных групп в течение 10 сут содержались на искусственном ярком освещении (150 лк) либо полном затемнении (2–3 лк). Моделью физического переутомления выбрана методика принудительного плавания крыс до полного утомления в собственной модификации. Через 24 ч после плавательного теста у всех групп животных оценивали поведенческую активность в «открытом поле» в условиях естественного освещения.Результаты. Установлено, что в контрольной группе в условиях естественного освещения через 1 сут после 5 сут ежедневной физической нагрузки происходило угнетение активно-поисковой составляющей поведения в «открытом поле», что выражалось в уменьшении количества пересеченных квадратов и вертикальных стоек в сравнении с интактными животными, не получавшими нагрузок. В группах животных, содержавшихся до предъявления плавательного теста в условиях круглосуточной темновой или световой деприваций, наблюдалось увеличение пассивно-оборонительной компоненты поведения в «открытом поле», что выражалось в увеличении актов груминга и дефекаций.Выводы. Следовательно, после 5 сут ежедневной физической нагрузки у крыс наблюдалось угнетение активно-поисковой составляющей поведения в «открытом поле». В течение 10 сут световой или темновой деприваций и последующего предъявления плавательного теста наблюдалось увеличение пассивно-оборонительной компоненты поведения животных в «открытом поле».

ЛОКАЛЬНЫЕ И СИСТЕМНЫЕ ЭФФЕКТЫ ИМПЛАНТАТОВ ГИАЛУРОНОВОЙ КИСЛОТЫ ПРИ КОРРЕКЦИИ ВОЗРАСТНЫХ ИЗМЕНЕНИЙ КОЖИ

Local and system influence the Repleri gel implants on the base of hyaluronic acid used to correct age-related changes in skin has been studied. Human stromal stem cells with CD44 membrane receptor to hyaluronic acid migrates in vitro into gel structure and maturates morphologically in fibroblast-like forms stained positively with acid phosphatase. Repleri gels implantation in the group of women with local and system age-related changes in homeostasis has positive local effect connected with full deletion of nasolabial fold in facial skin. Repleri gels injection has been decreasing during 1 year the system indices of real biological age as compared with chronological (passport) one. Lipoproteins and aspartate aminotransferase concentrations diminished statistically in women blood. An effect revealed may be considered as potential way of anti-age therapy of not only skin but parenchymal organs. At the same time, a long-term control of homeostasis distant indices in women with serious changes in parenchymal organs is necessary after local Repleri gels injection because of organism’s hormonal reactions.Изучено локальное и системное влияние гелевых имплантатов Repleri на основе гиалуроновой кислоты, применяемой для коррекции возрастных изменений кожи. Стромальные стволовые клетки человека, несущие рецептор CD44 к гиалуроновой кислоте, мигрируют in vitro в структуру геля и морфологически созревают в фибробластоподобные формы, позитивно окрашивающиеся на кислую фосфатазу. Имплантация гелей Repleri в группе женщин с локальными и системными возрастными изменениями гомеостаза дает положительный местный эстетический эффект, связанный с полным сглаживанием носогубной складки кожи лица. Введение гелей Repleri в течение 1 года снижало системные показатели реального биологического возраста в сравнении с хронологическим (паспортным). В крови пациенток статистически значимо уменьшались концентрации липопротеинов и аспартатаминотрансферазы. Обнаруженный эффект можно рассматривать в качестве потенциального способа антивозрастной терапии не только кожи, но и паренхиматозных органов. В то же время в связи с гормональными реакциями организма после местного назначения гелей Repleri требуется длительный контроль системных показателей гомеостаза у женщин с серьезными изменениями внутренних органов

ДИНАМИКА IN VITRO ДЕГРАДАЦИИ НЕТКАНЫХ МАТРИКСОВ ИЗ ПОЛИМОЛОЧНОЙ КИСЛОТЫ В МОДЕЛЬНОЙ БИОЛОГИЧЕСКОЙ ЖИДКОСТИ

The weekly in vitro degradation of fibrous-porous non-woven polylactide scaffolds made by aerodynamic formation in a turbulent gas flow has been studied with 37 °С in model RPMI-1640 medium imitated body fluid of organism. Lactate monomers released into solution exponentially and reached slowly a maximum value the end of the observation (5th week of dissolution). At the same time, reducing the concentrations of calcium and inorganic phosphorus ions in solutions contacted with tested samples (10×10×1 mm2) testified about chemical elements adsorption on artificial material. Ions exchange with biological fluids may be a basis of regulated bioactivity of fibrous-porous non-woven biodegradable material in application to intercellular matrix bioengineering for regenerative medicineИзучена понедельная деградация in vitro при температуре 37 °С волокнисто-пористых нетканых скеффолдов из полимолочной кислоты, полученных аэродинамическим формированием в турбулентном газовом потоке, в модельной среде RPMI-1640, имитирующей телесную жидкость организма. Мономеры молочной кислоты выделялись в раствор по экспоненциальному закону и медленно достигали максимальных концентраций к концу наблюдений (5-я нед растворения). В то же время снижение концентраций ионов кальция и неорганического фосфора в растворах, контактирующих с тестируемыми образцами (10 ´ 10 ´ 1 мм2 ), свидетельствовало об адсорбции химических элементов на искусственном материале. Обмен ионами с биологическими жидкостями могут быть основой регулируемой биоактивности волокнисто-пористого биодеградируемого материала в приложении к биоинженерии межклеточного матрикса для регенеративной медицины

BEHAVIORAL ACTIVITY OF RATS IN THE «OPEN FIELD» AFTER THE LIGHT AND DARK DEPRIVATION AND PHYSICAL EXHAUSTION

Aim. As is known, various stressful loads and their combination lead to unequal direction and degree of psychological and emotional instability. In this regard, one of the pressing issues becomes regulation and correction of psycho-emotional conditions of the person in the complicated conditions of activity, such as athletes during training and competition. To develop appropriate stress-corrective programs should their experimental validation. Therefore, the aim of this study was to investigate the behavioral activity of rats in the “open field” after dark or light deprivation and physical fatigue.Materials and methods. The experimental study was performed on the 40 adult male rats “Wistar”. The experimental groups for 10 days were kept in an artificial bright light (150 lx) or darkness (2–3 lx) for the induction of desynchronozes. Method of forced swimming until complete exhaustion was chosen for the model of physical fatigue. The animals in all groups evaluated behavioral activity in the “open field” in daylight conditions after 24 h after swim test.Results. It was established that in the control group in the terms of natural lighting day after 5 days of daily physical activity occurred depression of the active-search behavior in the “open field”. It was expressed in reducing the number of crossed squares and vertical struts in comparison to intact animals receiving no load. In the groups of animals, which kept in a dark or light deprivation until the presentation of the swim test there was an increase in passive-defensive behavior in the “open field”, which was reflected in an increase in acts of grooming and defecation

Adaptive reactions of rats after light desynchronosis and physical overwork

It is known that desynchronosis and physical stress to the point of fatigue are powerful stressors for the body. Studies indicate a depleting effect of the combined stress factors on the adaptive reserves of the organism, especially when the joint exposure is prolonged. However, the adaptive value of the training process in various activities is also well known. In this regard, it seems important to study these two aspects of adaptation when combined effects of light desynchronosis and physical overwork on the body.Рurpose of this study was to study the features of adaptive reactions of rats under conditions of light desynchronosis and physical overwork.Materials and methods. An experimental study was performed on 60 mature male rats of the Wistar breed. For the induction of experimental desynchronosis, the animals of the experimental groups were kept for 10 days in artificial bright illumination (150 LX) or full darkening (2-3 LX). The model of physical overfatigue used the method of forced swimming of rats to complete fatigue in their own modification. At the end of the swim test, all the animals were withdrawn from the experiment and underwent one-step-long decapitation under CO2 with anesthesia. In decapitated animals, to collect serum, the blood was collected in a clean, dry vial. In the blood serum of rats, the biochemical standard method was used to determine the level of lactate. Using the ELISA method and the reagent kit “IBL” (Germany), the concentrations of corticosterone and serotonin were determined. The histological analysis of the stress-realizing organs was carried out according to a standard procedure using light microscopy using the Axioskop 40 microscope from CarlZeiss (Germany).Results. It was found that physical fatigue in rats after daily swimming activities (5 days) was accompanied by a decrease in the level of corticosterone in the blood serum and destructive changes in the adrenal and femoral muscles in comparison with intact animals. In comparison with the intact group, dark deprivation and physical activity did not alter the content of corticosterone, serotonin, and lactate in the blood and caused minor destructive processes in the adrenal glands. Light deprivation and fatigue lowered the level of corticosterone in the blood, caused destructive changes in the adrenal glands and muscles, and increased serotonin levels in serum, but did not change the level of lactate.Conclusions. The features of adaptive reactions of rats under conditions of light desynchronosis and physical overfatigue are determined by the nature of deprivation or the direction of the phase shift (deprivation of light or darkness). Dark deprivation followed by physical overfatigue causes the development of the resistance phase of the general adaptation syndrome, and light deprivation followed by physical overwork is the phase of exhaustion

THE DYNAMICS OF IN VITRO DEGRADATION OF NON-WOVEN POLYLACTIDE MATRICES IN MODEL BIOLOGICAL LIQUID

The weekly in vitro degradation of fibrous-porous non-woven polylactide scaffolds made by aerodynamic formation in a turbulent gas flow has been studied with 37 °С in model RPMI-1640 medium imitated body fluid of organism. Lactate monomers released into solution exponentially and reached slowly a maximum value the end of the observation (5th week of dissolution). At the same time, reducing the concentrations of calcium and inorganic phosphorus ions in solutions contacted with tested samples (10×10×1 mm2) testified about chemical elements adsorption on artificial material. Ions exchange with biological fluids may be a basis of regulated bioactivity of fibrous-porous non-woven biodegradable material in application to intercellular matrix bioengineering for regenerative medicin