11 research outputs found
Rôle de l’AMPK, mTOR et GCN2 dans la détection des AA dans des hépatocytes en culture primaire
International audienc
A recombinant antigen-based lgG4 ELSA for the specific and sensitive detection of Brugia malayi infection
An IgG4 ELISA based on a novel recombinant antigen was evaluated for detection of Brugia malayi
infection, using 2487 sera from various institutions: 2031 samples from Universiti Sains Malaysia, 276
blinded sera from 2 other institutions in Malaysia, 140 blinded sera from India and 40 blinded sera from
Thailand. These sera were from various groups of individuals, i.e., microfilaraemics, chronic patients,
endemic normals, non-endemic normals and individuals with other parasitic and bacterial infections. Based
on a cut-off optical density reading of 0.300, the IgG4 ELISA demonstrated specificity rates of 95.6- l00%,
sensitivity rates of 96- l00%, positive predictive values of 75- 100% and negative predictive values of 98.9-
100%. These evaluation studies demonstrated the high specificity and sensitivity of this test for the detection
of active B. malayi infection. Thus, the IgG4 ELISA would be very useful as a tool in diagnosis and in
elimination programmes for brugian filariasis
AMPK inhibition in health and disease
All living organisms depend on dynamic mechanisms that repeatedly reassess the status of amassed energy, in order to adapt energy supply to demand. The AMP-activated protein kinase (AMPK) alpha beta gamma heterotrimer has emerged as an important integrator of signals managing energy balance. Control of AMPK activity involves allosteric AMP and ATP regulation, auto-inhibitory features and phosphorylation of its catalytic (alpha) and regulatory (beta and gamma) subunits. AMPK has a prominent role not only as a peripheral sensor but also in the central nervous system as a multifunctional metabolic regulator. AMPK represents an ideal second messenger for reporting cellular energy state. For this reason, activated AMPK acts as a protective response to energy stress in numerous systems. However, AMPK inhibition also actively participates in the control of whole body energy homeostasis. In this review, we discuss recent findings that support the role and function of AMPK inhibition under physiological and pathological states