Synthesis, Characterization, and Cytotoxicity of Iron Oxide Nanoparticles

In order to study the response of human breast cancer cells' exposure to nanoparticle, iron oxide (α-Fe2O3) nanoparticles were synthesized by a simple low temperature combustion method using Fe(NO3)3·9H2O as raw material. X-ray diffraction studies confirmed that the resultant powders are pure α-Fe2O3. Transmission electron microscopy study revealed the spherical shape of the primary particles, and the size of the iron oxide nanoparticles is in the range of 19 nm. The magnetic hysteresis loops demonstrated that the sample exposed ferromagnetic behaviors with a relatively low coercivity. The cytotoxicity of α-Fe2O3 nanoparticle was also evaluated on human breast cancer cells to address the current deficient knowledge of cellular response to nanoparticle exposure

<it>In vitro </it>cytotoxicity of <it>Strobilanthes crispus </it>ethanol extract on hormone dependent human breast adenocarcinoma MCF-7 cell

<p>Abstract</p> <p>Background</p> <p><it>Strobilanthes crispus </it>has been traditionally used as antidiabetic, anticancer, diuretic, antilytic and laxative agent. However, cytotoxicity and antiproliferative effect of <it>S. crispus </it>is still unclear.</p> <p>Results</p> <p><it>Strobilanthes cripus </it>was able to reduce cell viability and proliferation in MTT and BrdU assays. Both cell cycle progression and Tunel assay suggested that IC₅₀of <it>S. crispus </it>ethanol extract induced sub-G1 cell cycle phase, and DNA fragmentation. On the other hand, translocation of mitochondria cytochrome c release, induction of caspase 3/7 and p53 while suppress XIAP on treated MCF-7 cell were also observed in this study.</p> <p>Conclusion</p> <p>Our findings suggest that <it>S. crispus </it>ethanol extract induced apoptosis and DNA fragmentation on hormone dependent breast cancer cell line MCF-7 via mitochondria dependent p53 apoptosis pathway.</p