Determination of Iron (II), Iron (III) and Total Iron in Some β-Thalassemia Patients using Different Analytical Techniques

There are many well-known analytical methods for determination of iron (II) and iron (III). A mong these methods: gravimetric, titrimetric, potentiometric, conductometric, batch and flow – injection spectrophotometric methods. In the present study, two batch spectrophotometric, atomic absorption spectrometric and biolabo kit methods have been used for determination of iron (II), iron (III) and total iron. The present methods have the advantages of high sensitivity, low cost reagent, low operation cost, simplicity, speed and their applications for determination of iron (II) and iron (III) in some serum samples of normal human and β-thallasemia patients in Erbil city. For the first time especially in Erbil city attempts were made to use zero, first and second derivative spectra to identify the serum samples of some β-thallasemia patients from the normal human serum samples due to the appearance and resolution of peaks in both cases

Determination of Iron (II), Iron (III) and Total Iron in Some β-Thalassemia Patients using Different Analytical Techniques

There are many well-known analytical methods for determination of iron (II) and iron (III). A mong these methods: gravimetric, titrimetric, potentiometric, conductometric, batch and flow – injection spectrophotometric methods. In the present study, two batch spectrophotometric, atomic absorption spectrometric and biolabo kit methods have been used for determination of iron (II), iron (III) and total iron. The present methods have the advantages of high sensitivity, low cost reagent, low operation cost, simplicity, speed and their applications for determination of iron (II) and iron (III) in some serum samples of normal human and β-thallasemia patients in Erbil city. For the first time especially in Erbil city attempts were made to use zero, first and second derivative spectra to identify the serum samples of some β-thallasemia patients from the normal human serum samples due to the appearance and resolution of peaks in both cases

Molecular typing of Clostridioides difficile isolates from clinical and non-clinical samples in Iran

Clostridioides difficile is a major cause of nosocomial infectious diarrhea in hospitalized patients throughout the world. We aimed to characterize C. difficile isolates among hospitalized patients, hospital staffs, and hospital environment samples obtained in three tertiary care hospitals of Iran with regard to their molecular types between June 2016 and November 2017. The toxigenicity of C. difficile isolates was determined by toxigenic culture and multiplex-PCR. Toxigenic C. difficile isolates collected were ribotyped using capillary gel electrophoresis-based PCR and the database of WEBRIBO (http://webribo.ages.at). Of 500 clinical and non-clinical samples, toxigenic C. difficile were identified in 35 of 250 stool samples (14) and in 3 of 250 swabs (1.2). The most frequently found ribotypes (RTs) were 039, AI-12, and AI-21 (15.8, 10.52, and 10.52 of all isolates, respectively). Further RTs were: 017, 001, AI-3, AI-15, AI-18, AI-10, AI-4, and PR21195 (as new ribotype). The epidemic RTs (027 and 078) seen in the Europe, North America, and Asia were completely absent in this study. © 2019 APMIS. Published by John Wiley & Sons Lt