10,378 research outputs found
Electron microscopic visualization of tRNA genes with ferritin-avidin: biotin labels
A method is described for indirect electron microscopic visualization and mapping of tRNA and other short transcripts hybridized to DNA. This method depends upon the attachment of the electron-dense protein ferritin to the RNA, the binding being mediated by the remarkably strong association of the egg white protein avidin with biotin. Biotin is covalently attached to the 3' end of tRNA using an NH2 (CH2) 5NH2 bridge. The tRNA-biotin adduct is hybridized to complementcrry DNA sequences present in a single stranded nonhomology loop of a DNA:DNA heteroduplex. Avidin, covalently crosslinked to ferritin is mixed with the heteroduplex and becomes bound to the hybridized tRNA-biotin. Observation of the DNA:RNA-biotin:avidin-ferritin complex by electron microsdopy specifically and accurately reveals the position of the tRNA gene, with a frequency of labeling of approximately 50%
Ising metamagnets in thin film geometry: equilibrium properties
Artificial antiferromagnets and synthetic metamagnets have attracted much
attention recently due to their potential for many different applications.
Under some simplifying assumptions these systems can be modeled by thin Ising
metamagnetic films. In this paper we study, using both the Wang/Landau scheme
and importance sampling Monte Carlo simulations, the equilibrium properties of
these films. On the one hand we discuss the microcanonical density of states
and its prominent features. On the other we analyze canonically various global
and layer quantities. We obtain the phase diagram of thin Ising metamagnets as
a function of temperature and external magnetic field. Whereas the phase
diagram of the bulk system only exhibits one phase transition between the
antiferromagnetic and paramagnetic phases, the phase diagram of thin Ising
metamagnets includes an additional intermediate phase where one of the surface
layers has aligned itself with the direction of the applied magnetic field.
This additional phase transition is discontinuous and ends in a critical end
point. Consequently, it is possible to gradually go from the antiferromagnetic
phase to the intermediate phase without passing through a phase transition.Comment: 8 figures, accepted for publication in Physical Review
Extending Feynman's Formalisms for Modelling Human Joint Action Coordination
The recently developed Life-Space-Foam approach to goal-directed human action
deals with individual actor dynamics. This paper applies the model to
characterize the dynamics of co-action by two or more actors. This dynamics is
modelled by: (i) a two-term joint action (including cognitive/motivatonal
potential and kinetic energy), and (ii) its associated adaptive path integral,
representing an infinite--dimensional neural network. Its feedback adaptation
loop has been derived from Bernstein's concepts of sensory corrections loop in
human motor control and Brooks' subsumption architectures in robotics.
Potential applications of the proposed model in human--robot interaction
research are discussed.
Keywords: Psycho--physics, human joint action, path integralsComment: 6 pages, Late
Neoliberalism and authoritarian media cultures: a Vietnamese perspective
Nguyễn Yến-Khanh, Sean Phelan, Elizabeth Gray, "Neoliberalism and authoritarian media cultures: a Vietnamese perspective" First Published in " Media, Culture & Society" January 29, 2022. https://doi.org/10.1177/01634437211060200Publishe
The Stabilization of Superconductivity by Magnetic Field in Out-of-Equilibrium Nanowires
A systematic study has been carried out on the previously reported
"magnetic-field-induced superconductivity" of Zn nanowires. By varying
parameters such as magnetic field orientation and wire length, the results
provide evidence that the phenomenon is a nonequilibrium effect associated with
the boundary electrodes. They also suggest there are two length scales
involved, the superconducting coherence length and quasiparticle relaxation
length. As wire lengths approach either of these length scales, the effect
weakens. We demonstrate that it is appropriate to consider the effect to be a
stabilization of superconductivity, that has been suppressed by an applied
current.Comment: (Updated Version) 9 pages, 8 figure
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