Efek Pemberian Ekstrak Klorofil Daun Pepaya (Carica Papaya, Linn.) Terhadap Kadar Ast Dan Alt Serum

Background: Pharmacological therapy is one of the therapy modalities which carries the hepatic injury as one of its side effects. Nowadays, curcuma tablets has become choice of hepatoprotector. Other substance which has the possibilities of hepatoprotection activities is chlorophyll. The study is aimed to investigate the effect of papaya leaves chlorophyll extracts towards the serum AST and serum ALT levels on high-dose-paracetamol-induced Wistar rats. Materials and methods: This was an experimental study applying pretest-posttest controlled group design. Twenty eight Wistar rats 8-12 weeks age and weighted 185-220 grams assigned into four groups, namely group I (control), group II (paracetamol 750 mg/kg bw + no hepatoprotector agent), group III (paracetamol 750 mg/kg bw + curcuma 100 mg/kg bw), and group IV (paracetamol 750 mg/kg bw + papaya leaves chlorophyll extracts 300 mg/kg bw). The hepatoprotector agents were administered for a week, while the high dose paracetamol was administered for three consecutive days (day 5, 6, and 7). The pretest samples were drawn on the fifth day before the administration of high dose paracetamol, and the posttest samples were drawn on eightth day. Results: There were no difference among four groups on serum AST and serum ALT levels before the administration of high dose paracetamol, p=0.522 dan p=0.682, respectively. After the administration of high dose paracetamol, there were differences among four groups on both variables, both p=0.000. The post-hoc test showed that differences happened on all four groups (p<0.05).Conclusion: Chlorophyll extract of papaya leaves can inhibit liver injury on high-dose-paracetamol-induced-Wistar rats

Pengaruh Ekstrak Daun Kersen (Muntingia Calabura) Terhadap Gambaran Mikroskopis Hepar Tikus Wistar Jantan Yang Diinduksi Etanol Dan Soft Drink

Latar Belakang : Konsumsi alkohol dan soft drink semakin meningkat di dunia. Minuman yang mengandung etanol dan soft drink memiliki efek negatif terhadap hepar, diantaranya menghasilkan peroksidasi lipid, inflamasi hepar, stres oksidatif, penyakit hati alkoholik, resistensi insulin, dan Non Alcoholic Fatty Liver Disease. Ekstrak daun kersen memiliki sifat antioksidan dan antiinflamasi yang berpotensi sebagai hepatoprotektor sehingga dapat mencegah dan mengurangi kerusakan pada hepar.Tujuan: Membuktikan pengaruh ekstrak daun kersen terhadap gambaran mikroskopis hepar tikus Wistar jantan yang diinduksi etanol dan soft drink.Metode: Penelitian true experimental dengan Post Test-Only Control Group Design. Sebanyak 30 tikus Wistar jantan dibagi menjadi 5 kelompok yaitu kontrol, P1, P2, P3, dan P4. Kelompok P1 dan P3 diberi etanol 40% sebesar 1,8 ml/200 g/hari. Kelompok P2 dan P4 diberi soft drink sebesar 50 ml/hari. Kelompok P3 dan P4, 60 menit sebelum diinduksi etanol 40% dan soft drink diberikan ekstrak daun kersen sebesar 500 mg/kgBB/hari. Setelah intervensi 30 hari, sampel diterminasi dan heparnya dibuat preparat untuk diamati gambaran mikroskopis. Uji analisis menggunakan Kruskal-Wallis dan Mann Whitney.Hasil: Pemeriksaan histopatologi menunjukkan sebagian besar hepatosit dalam keadaan normal pada kelompok kontrol, nekrosis zona 3 lobulus hepar pada P1, degenerasi parenkimatosa pada P2, degenerasi parenkimatosa pada P3, dan hepatosit dalam keadaan normal pada P4. Uji Kruskal-Wallis menunjukkan perbedaan yang bermakna pada seluruh kelompok (Kontrol, P1, P2, P3, dan P4) dengan p=0,001. Hasil uji Mann-Whitney menunjukkan perbedaan yang bermakna pada K-P1 (p=0,001), K-P2 (p=0,001), P1-P3 (p=0,001), dan P2-P4 (p=0,011).Kesimpulan: Pemberian ekstrak daun kersen menghasilkan perbedaan yang bermakna pada gambaran mikroskopis hepar yang diinduksi etanol dan soft drink

Ekstrak Bawang Putih (Allium Sativum) dan Ekspresi Insulin Serta Derajat Insulitis Pankreas Tikus Sprague-Dawley yang Diinduksi Streptozotocin

Allium sativum, insulin expression and insulitis degree of pancreas Sprague-Dawley rat induced by streptozotocinBackground: The organic-sulphur content in Allium sativum has been scientifically proven to be a potent insulin-mimetic, antioxidant, and anti-inflammation agent by inhibiting the activity of NF-κβ. This research is aimed at finding out the effect of Allium sativum extract towards insulitis level and insulin expression in pancreas of Sprague-Dawley male rats.Method: This research used a randomized post test only controlled group design. There were 28 rats, two were randomly selected for the initial blood glucose test prior to treatment, two were selected on the tenth day after streptozotocin induction to ensure that all rats were in hyperglycaemia condition, and the remains as many as 24 rats were grouped into four groups: three treatmentgroups were administered with Allium sativum in levelled doses of 0.1, 0.25, 0.5 grams/kgBW/day for 14 days, and one group were act as control negative. Kruskall Wallis test continued with Mann Whitney test were used, with significance level of p<0.05.Results: The insulitis level and insulin expression of rats\u27 pancreas in the three treatment groups showed significant difference compared with the group without treatment showing dose-dependent result. However, it was not able to completely restore the damage pancreas.Conclusion: Allium sativum extract may play important role in restoring damage pancreas

Aktivitas Gel Ekstrak Kolagen Sisik Ikan Kakap Merah (Lutjanus Argentimaculatus) Terhadap Fase Epitelisasi Pada Proses Penyembuhan Luka Bakar Kulit Kelinci “Gambaran Makroskopis Dan Mikroskopis”

SARI Luka bakar adalah kerusakan jaringan yang disebabkan kontak dengan sumber panas. Kolagen merupakan material dominan yang berguna untuk regenerasi jaringan dan berpotensi untuk mempercepat penyembuhan luka bakar Penelitian ini bertujuan untuk mengetahui pengaruh perbedaan konsentrasi ekstrak kolagen sisik ikan kakap merah dalam sediaan gel terhadap karakteristik fisik gel, gambaran makroskopis dan mikroskopis fase epitelisasi pada proses penyembuhan luka bakar derajat II. Penelitian ini menggunakan 9 ekor kelinci jantan galur New Zealand usia 4-6 bulan, punggung kelinci dibagi menjadi 5 area luka yang diinduksi dengan lempeng besi panas berdiameter 20 mm dengan suhu 70oC selama 5 detik. Setiap kelompok diberikan perlakuan gel ekstrak kolagen konsentasi 0,3%, 0,6%, dan 0,9%. kontrol negatif diberikan perlakuan basis gel, sedangkan kelompok kontrol positif diberikan perlakuan gel Neomisin sulfat-Plasenta bovine. Pengamatan secara makroskopis dilakukan setiap hari dan pengamatan secara mikroskopis dilakukan pada hari ke-3, 9 dan 12. Data karakteristik fisik gel dianalisis menggunakan SPSS dengan taraf kepercayaan 95% menunjukkan perbedaan signifikan (P&lt;0,05) antara gel ekstrak kolagen terhadap basis gel. Pengaruh ekstrak kolagen terhadap karakteristik fisik gel yaitu dapat menurunkan pH, viskositas, daya lekat, dan meningkatkan daya sebar. Hasil uji statistik aktivitas gel ekstrak kolagen menunjukkan tidak berbeda signifikan secara makroskopis, namun hasil skoring fase epitelisasi luka bakar kulit kelinci menunjukkan perbedaan signifikan dengan kontrol negatif. Hasil tersebut menunjukkan bahwa pemberian gel ekstrak kolagen sisik ikan kakap merah dapat mempercepat fase epitelisasi pada proses penyembuhan luka bakar

Luaran Maternal Dan Perinatal Pada Ibu Hamil Dengan Preeklampsia Berat

Background:Maternal mortality rate (MMR) in Indonesia in 2013 was 190 / 100,000 live births and 101.92 / 100,000 live births in Semarang in 2010. Infant Mortality Rate (IMR) in Indonesia reached 34/1000 live births. Severe preeclampsia is one cause of high MMR and IMR. Severe preeclampsia also increases the incidence of maternal and perinatal morbidity,thus it is necessary to do research on maternal and perinatal outcomes in women with severe preeclampsia.Aim:To describe the maternal and perinatal outcome of pregnant women with severe preeclampsia and to analyze the difference between maternal and perinatal outcomes of pregnant women with severe preeclampsia who have and don\u27t have a history of preeclampsia.Method:This study was an analytic observational research which used cross sectional method and secondary data, which was medical records. The main concern of this study was the characteristic, maternal, and perinatal outcome analyzed descriptively. Then, the differences between the outcome of women with and without history of preeclampsia was analyzed with fischer exact.Result:Maternal characteristics which appears the most is the number of multiparous mothers 40.7%. While maternal and perinatal outcomes that appear most frequently are impaired vision and low birth weight, as much as 42.4% and 49.2%. The difference between outcomes in women who have and do not have a history of preeclampsia is not significant.Conclusion: There is no significant difference between maternal and perinatal outcomes in women who have and do not have a history of preeclampsia

Perbandingan Pelayanan Kesehatan Pada Pasien Kelas 1 Dengan VIP Di Pusat Pelayanan Jantung Dan Pembuluh Darah RSUP Dr Kariadi Semarang.

Background : Hospital is a unit that has a regular organization, where prevention and cure of disease, health improvement and rehabilitation patients in a multidisciplinary done by various groups of skilled and educated professionals, used the infrastructure and physical facilities. Hospitalization room in Dr. Kariadi Hospital have some form class, they are third class, second class, first class and Very Important Person (VIP class. First class and VIP class services should be, in accordance with the Standard Minimum (SPM), same although with some different facilities. But the reality may be difference.Aim : To know whether the health service given to the patient in the hospitalization room in the Heart Service Unit of Dr. Kariadi Hospital was same between VIP class and first class.Method : This was an observational analytic study with cross-sectional study. The samples were obtained by taking respondents with consecutive sampling method until the end of the research (from May to July 2012). The gathered descriptive primary data were presented in the table form with images and diagrams. The data were analyzed using Fisher exact test.Results : The interpretation data of each class were analyzed using Fisher exact test but the result was not significant (p=0.49).Conclusion : There was no meaningful difference in the provided services between VIP class and first class in the Heart Service Unit of Dr. Kariadi Hospital Semarang