123 research outputs found
Protein carbonylation as a mechanism of regulation of MCF-7 breast cancer cell proliferation
Tumor progression is accompanied by dysregulation of cell proliferation and apoptosis, which plays an essential role in breast cancer pathogenesis and cell resistance to chemotherapy. The role of protein carbonylation in molecular mechanisms of regulating MCF-7 breast cancer cell proliferation under the effect of roscovitine, a selective inhibitor of cyclin-dependent kinases, was evaluated. The cells were grown in adherent cell culture with or without roscovitine. The levels of reduced/oxidized glutathione and the concentration of protein carbonyl derivatives were determined by spectrophotometry. The cell cycle was evaluated by the flow cytometry; the same technique was used to measure the reactive oxygen species (ROS) concentration. Cell culture with roscovitine resulted in a decrease in the redox potential of the glutathione system and a rise in the ROS and protein carbonyl derivative concentrations. Roscovitine contributed to the G0/G1 and G2/Π phase arrest due to its interaction with ATP-binding sites of cyclin-dependent kinases. Roscovitine could also promote enzyme carbonylation. The obtained results can be further used for development of personalized approaches to breast cancer therapy
ΠΠ½Π°Π»ΠΈΠ· ΡΠΎΠ²ΠΌΠ΅ΡΡΠ½ΠΎΠ³ΠΎ Π²Π»ΠΈΡΠ½ΠΈΡ ΠΏΠΎΠ»ΠΈΠΌΠΎΡΡΠΈΠ·ΠΌΠΎΠ² Π³Π΅Π½ΠΎΠ² ΡΠΈΡΡΠ΅ΠΌΡ ΠΈΠ½ΡΠ΅ΡΡΠ΅ΡΠΎΠ½Π° OAS1, OAS3, PKR, IFNA17 ΠΈ IFNG Π½Π° ΠΏΡΠ΅Π΄ΡΠ°ΡΠΏΠΎΠ»ΠΎΠΆΠ΅Π½Π½ΠΎΡΡΡ ΠΊ Ρ ΡΠΎΠ½ΠΈΡΠ΅ΡΠΊΠΎΠΌΡ Π²ΠΈΡΡΡΠ½ΠΎΠΌΡ Π³Π΅ΠΏΠ°ΡΠΈΡΡ C
Interferon system plays a key role in the antiviral response. Based on data from the literature, the most dangerous for the development of infectious diseases is the combination of risk alleles of several genes. This study focuses on the analysis of the combined effect of single nucleotide polymorphisms of interferon system genes OAS1, OAS3, PKR, IFNA17 and IFNG in the predisposition to chronic viral hepatitis C. It has been shown that the accounting of several risk genes improves the prediction of development of this disease.Π‘ΠΈΡΡΠ΅ΠΌΠ° ΠΈΠ½ΡΠ΅ΡΡΠ΅ΡΠΎΠ½Π° ΠΈΠ³ΡΠ°Π΅Ρ ΠΊΠ»ΡΡΠ΅Π²ΡΡ ΡΠΎΠ»Ρ Π² ΡΠ΅Π°Π»ΠΈΠ·Π°ΡΠΈΠΈ ΠΏΡΠΎΡΠΈΠ²ΠΎΠ²ΠΈΡΡΡΠ½ΠΎΠ³ΠΎ ΠΎΡΠ²Π΅ΡΠ°. ΠΠ·Π²Π΅ΡΡΠ½ΠΎ, ΡΡΠΎ Π½Π°ΠΈΠ±ΠΎΠ»Π΅Π΅ ΠΎΠΏΠ°ΡΠ½ΡΠΌ Π΄Π»Ρ Π²ΠΎΠ·Π½ΠΈΠΊΠ½ΠΎΠ²Π΅Π½ΠΈΡ ΠΈΠ½ΡΠ΅ΠΊΡΠΈΠΎΠ½Π½ΡΡ
Π±ΠΎΠ»Π΅Π·Π½Π΅ΠΉ ΡΠ²Π»ΡΠ΅ΡΡΡ ΡΠΎΡΠ΅ΡΠ°Π½ΠΈΠ΅ ΡΠΈΡΠΊΠΎΠ²ΡΡ
Π°Π»Π»Π΅Π»Π΅ΠΉ Π½Π΅ΡΠΊΠΎΠ»ΡΠΊΠΈΡ
Π³Π΅Π½ΠΎΠ².Π Π°Π±ΠΎΡΠ° ΠΏΠΎΡΠ²ΡΡΠ΅Π½Π° Π°Π½Π°Π»ΠΈΠ·Ρ ΡΠΎΠ²ΠΌΠ΅ΡΡΠ½ΠΎΠ³ΠΎ Π²Π»ΠΈΡΠ½ΠΈΡ ΠΎΠ΄Π½ΠΎΠ½ΡΠΊΠ»Π΅ΠΎΡΠΈΠ΄Π½ΡΡ
ΠΏΠΎΠ»ΠΈΠΌΠΎΡΡΠΈΠ·ΠΌΠΎΠ² Π³Π΅Π½ΠΎΠ² ΡΠΈΡΡΠ΅ΠΌΡ ΠΈΠ½ΡΠ΅ΡΡΠ΅ΡΠΎΠ½Π° OAS1, OAS3, PKR, IFNA17 ΠΈ IFNG Π½Π° ΠΏΡΠ΅Π΄ΡΠ°ΡΠΏΠΎΠ»ΠΎΠΆΠ΅Π½Π½ΠΎΡΡΡ ΠΊ Ρ
ΡΠΎΠ½ΠΈΡΠ΅ΡΠΊΠΎΠΌΡ Π²ΠΈΡΡΡΠ½ΠΎΠΌΡ Π³Π΅ΠΏΠ°ΡΠΈΡΡ Π‘. ΠΠΎΠΊΠ°Π·Π°Π½ΠΎ, ΡΡΠΎ ΡΡΠ΅Ρ Π½Π΅ΡΠΊΠΎΠ»ΡΠΊΠΈΡ
ΡΠΈΡΠΊΠΎΠ²ΡΡ
Π³Π΅Π½ΠΎΠ² ΡΠ»ΡΡΡΠ°Π΅Ρ ΠΏΡΠΎΠ³Π½ΠΎΠ·ΠΈΡΠΎΠ²Π°Π½ΠΈΠ΅ ΡΠ°Π·Π²ΠΈΡΠΈΡ Π΄Π°Π½Π½ΠΎΠ³ΠΎ Π·Π°Π±ΠΎΠ»Π΅Π²Π°Π½ΠΈΡ
ΠΠΎΠ»Π΅ΠΊΡΠ»ΡΡΠ½ΡΠ΅ ΠΎΡΠ½ΠΎΠ²Ρ Π΄ΠΈΠ·ΡΠ΅Π³ΡΠ»ΡΡΠΈΠΈ ΠΏΡΠΎΠ³ΡΠ°ΠΌΠΌΠΈΡΠΎΠ²Π°Π½Π½ΠΎΠΉ Π³ΠΈΠ±Π΅Π»ΠΈ Π»ΠΈΠΌΡΠΎΡΠΈΡΠΎΠ² ΠΏΡΠΈ Ρ ΡΠΎΠ½ΠΈΡΠ΅ΡΠΊΠΎΠΉ Π²ΠΈΡΡΡΠ½ΠΎΠΉ ΠΈΠ½ΡΠ΅ΠΊΡΠΈΠΈ
The review analyses information from recent literature and results of the authorsβ own investigations concerning imbalance of programmed cell death in forming chronic viral infection. Molecular mechanisms of apoptosis modulation of immune cells by persistent viruses are discussed in the article.Π ΡΡΠ°ΡΡΠ΅ Π°Π½Π°Π»ΠΈΠ·ΠΈΡΡΡΡΡΡ Π΄Π°Π½Π½ΡΠ΅ ΡΠΎΠ²ΡΠ΅ΠΌΠ΅Π½Π½ΠΎΠΉ Π»ΠΈΡΠ΅ΡΠ°ΡΡΡΡ ΠΈ ΡΠ΅Π·ΡΠ»ΡΡΠ°ΡΡ ΡΠΎΠ±ΡΡΠ²Π΅Π½Π½ΡΡ
ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠΉ ΠΎ ΡΠΎΠ»ΠΈ Π΄ΠΈΡΠ±Π°Π»Π°Π½ΡΠ° ΠΏΡΠΎΠ³ΡΠ°ΠΌΠΌΠΈΡΠΎΠ²Π°Π½Π½ΠΎΠΉ ΠΊΠ»Π΅ΡΠΎΡΠ½ΠΎΠΉ Π³ΠΈΠ±Π΅Π»ΠΈ Π² ΡΠΎΡΠΌΠΈΡΠΎΠ²Π°Π½ΠΈΠΈ Ρ
ΡΠΎΠ½ΠΈΡΠ΅ΡΠΊΠΎΠΉ Π²ΠΈΡΡΡΠ½ΠΎΠΉ ΠΈΠ½ΡΠ΅ΠΊΡΠΈΠΈ. ΠΠ±ΡΡΠΆΠ΄Π°ΡΡΡΡ ΠΌΠΎΠ»Π΅ΠΊΡΠ»ΡΡΠ½ΡΠ΅ ΠΌΠ΅Ρ
Π°Π½ΠΈΠ·ΠΌΡ ΠΌΠΎΠ΄ΡΠ»ΠΈΡΠΎΠ²Π°Π½ΠΈΡ ΡΠ΅Π°Π»ΠΈΠ·Π°ΡΠΈΠΈ Π°ΠΏΠΎΠΏΡΠΎΠ·Π° ΠΈΠΌΠΌΡΠ½ΠΎΠΊΠΎΠΌΠΏΠ΅ΡΠ΅Π½ΡΠ½ΡΡ
ΠΊΠ»Π΅ΡΠΎΠΊ ΠΏΠ΅ΡΡΠΈΡΡΠΈΡΡΡΡΠΈΠΌΠΈ Π²ΠΈΡΡΡΠ°ΠΌΠΈ
Π€ΠΈΠ·ΠΈΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠ°Ρ ΡΠ΅ΠΏΠ°ΡΠ°ΡΠΈΡ ΡΡΠΈΡΡΠΎΡΠΈΡΠΎΠ² Π½Π° ΡΡΠΎΠ²Π½Π΅ Π΄ΡΠ³ΠΈ Π°ΠΎΡΡΡ
The erythrocyte distribution at the level of aortic arch is determined experimentally from structural-functional features. It is shown that there exists the mechanism of physiological separation of erythrocytes, which causes the prevalent income of younger and functionally valid forms of erythrocytes to brain and favors better oxygen supply of nerve cells.ΠΠΊΡΠΏΠ΅ΡΠΈΠΌΠ΅Π½ΡΠ°Π»ΡΠ½ΠΎ ΡΡΡΠ°Π½ΠΎΠ²Π»Π΅Π½ΠΎ ΡΠ°ΡΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ΠΈΠ΅ ΡΡΠΈΡΡΠΎΡΠΈΡΠΎΠ² Π½Π° ΡΡΠΎΠ²Π½Π΅ Π΄ΡΠ³ΠΈ Π°ΠΎΡΡΡ ΠΏΠΎ ΡΡΡΡΠΊΡΡΡΠ½ΠΎ-ΡΡΠ½ΠΊΡΠΈΠΎΠ½Π°Π»ΡΠ½ΡΠΌ ΠΏΡΠΈΠ·Π½Π°ΠΊΠ°ΠΌ. ΠΠΎΠΊΠ°Π·Π°Π½ΠΎ ΡΡΡΠ΅ΡΡΠ²ΠΎΠ²Π°Π½ΠΈΠ΅ ΠΌΠ΅Ρ
Π°Π½ΠΈΠ·ΠΌΠ° ΡΠΈΠ·ΠΈΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΎΠΉ ΡΠ΅ΠΏΠ°ΡΠ°ΡΠΈΠΈ ΡΡΠΈΡΡΠΎΡΠΈΡΠΎΠ², ΠΊΠΎΡΠΎΡΡΠΉ ΠΎΠ±ΡΡΠ»ΠΎΠ²Π»ΠΈΠ²Π°Π΅Ρ ΠΏΡΠ΅ΠΈΠΌΡΡΠ΅ΡΡΠ²Π΅Π½Π½ΠΎΠ΅ ΠΏΠΎΡΡΡΠΏΠ»Π΅Π½ΠΈΠ΅ Π±ΠΎΠ»Π΅Π΅ ΠΌΠΎΠ»ΠΎΠ΄ΡΡ
ΠΈ ΡΡΠ½ΠΊΡΠΈΠΎΠ½Π°Π»ΡΠ½ΠΎ ΠΏΠΎΠ»Π½ΠΎΡΠ΅Π½Π½ΡΡ
ΡΠΎΡΠΌ ΡΡΠΈΡΡΠΎΡΠΈΡΠΎΠ² Π² Π³ΠΎΠ»ΠΎΠ²Π½ΠΎΠΉ ΠΌΠΎΠ·Π³ ΠΈ ΡΠΏΠΎΡΠΎΠ±ΡΡΠ²ΡΠ΅Ρ ΡΠ»ΡΡΡΠ΅Π½ΠΈΡ ΡΠ½Π°Π±ΠΆΠ΅Π½ΠΈΡ ΠΊΠΈΡΠ»ΠΎΡΠΎΠ΄ΠΎΠΌ Π½Π΅ΡΠ²Π½ΡΡ
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